AB87775

Anti-SOX1 antibody

Name: Anti-SOX1 antibody (ab87775) | Abcam
Brand: Abcam Limited
SKU: AB87775
Rating: 3 (10 reviews)

(10 Reviews)

(32 Publications)

Rabbit Polyclonal SOX1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 32 publications.

View Alternative Names

Transcription factor SOX-1, SOX1

2 Images

Lab

Western blot - Anti-SOX1 antibody (AB87775)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA before being incubated with ab87775 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab6721), and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-SOX1 antibody (ab87775) at 1 µg/mL

Lane 1:

E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

Lane 2:

46C (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa,45 kDa

true

Exposure time: 8min

AbReview33158****

Western blot - Anti-SOX1 antibody (AB87775)

All lanes:

Western blot - Anti-SOX1 antibody (ab87775) at 1/1000 dilution

Lane 1:

H1 Human embryonic stem cells at 20 µg

Lane 2:

H1 Human embryonic stem cell derived neural progenitor stem cells at 20 µg

Secondary

All lanes:

Mouse anti-rabbit HRP conjugated at 1/20000 dilution

Predicted band size: 39 kDa

Observed band size: 50 kDa

true

Exposure time: 30s

This image is courtesy of an abreview by Patrick Ovando Roche

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

See full target information SOX1

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70958 PubMed40873389

2025

Intracellular and Extracellular Vesicle miRNA Signatures in Human iPSC-Derived Neural Stem Cells and Floor Plate Progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Lilian Cruz,Frederico Moraes Ferreira,Camila Miranda Lopes-Ramos,Zhiyun Wei,William T Hendriks,H A Jinnah,Helder I Nakaya,D Cristopher Bragg,Anna M Krichevsky,Xandra Owens Breakefield,Marilene Hohmuth Lopes

Neural regeneration research 20:518-532 PubMed38819064

2024

Human-induced pluripotent stem cell-derived neural stem cell exosomes improve blood-brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Conglin Wang,Fangyuan Cheng,Zhaoli Han,Bo Yan,Pan Liao,Zhenyu Yin,Xintong Ge,Dai Li,Rongrong Zhong,Qiang Liu,Fanglian Chen,Ping Lei

Neurobiology of disease 194:106474 PubMed38518837

2024

Engrailed 1 deficiency induces changes in ciliogenesis during human neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Hembach,Sebastian Schmidt,Tanja Orschmann,Ingo Burtscher,Heiko Lickert,Florian Giesert,Daniela Vogt Weisenhorn,Wolfgang Wurst

Nature communications 14:7674 PubMed37996418

2023

A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Schmidt,Constantin Stautner,Duc Tung Vu,Alexander Heinz,Martin Regensburger,Ozge Karayel,Dietrich Trümbach,Anna Artati,Sabine Kaltenhäuser,Mohamed Zakaria Nassef,Sina Hembach,Letyfee Steinert,Beate Winner,Winkler Jürgen,Martin Jastroch,Malte D Luecken,Fabian J Theis,Gil Gregor Westmeyer,Jerzy Adamski,Matthias Mann,Karsten Hiller,Florian Giesert,Daniela M Vogt Weisenhorn,Wolfgang Wurst

eLife 12: PubMed37466241

2023

Regulation of store-operated Ca entry by IP receptors independent of their ability to release Ca.

Applications

Unspecified application

Species

Unspecified reactive species

Pragnya Chakraborty,Bipan Kumar Deb,Vikas Arige,Thasneem Musthafa,Sundeep Malik,David I Yule,Colin W Taylor,Gaiti Hasan

International journal of molecular sciences 24: PubMed37108456

2023

An Efficient 2D Protocol for Differentiation of iPSCs into Mature Postmitotic Dopaminergic Neurons: Application for Modeling Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Olga S Lebedeva,Elena I Sharova,Dmitriy A Grekhnev,Liubov O Skorodumova,Irina V Kopylova,Ekaterina M Vassina,Arina Oshkolova,Iuliia V Novikova,Alena V Krisanova,Evgenii I Olekhnovich,Vladimir A Vigont,Elena V Kaznacheyeva,Alexandra N Bogomazova,Maria A Lagarkova

International journal of molecular sciences 23: PubMed36555735

2022

PLEKHM2 Loss of Function Impairs the Activity of iPSC-Derived Neurons via Regulation of Autophagic Flux.

Applications

Unspecified application

Species

Unspecified reactive species

Hadas Ben-Zvi,Tatiana Rabinski,Rivka Ofir,Smadar Cohen,Gad D Vatine

Nature communications 13:4819 PubMed35974013

2022

Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Schmidt,Malte D Luecken,Dietrich Trümbach,Sina Hembach,Kristina M Niedermeier,Nicole Wenck,Klaus Pflügler,Constantin Stautner,Anika Böttcher,Heiko Lickert,Ciro Ramirez-Suastegui,Ruhel Ahmad,Michael J Ziller,Julia C Fitzgerald,Viktoria Ruf,Wilma D J van de Berg,Allert J Jonker,Thomas Gasser,Beate Winner,Jürgen Winkler,Daniela M Vogt Weisenhorn,Florian Giesert,Fabian J Theis,Wolfgang Wurst

The Journal of biological chemistry 298:102106 PubMed35671824

2022

Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Yang Liu,Lihua Shi,Lei Fang,Liyang Xu,Ying Cao

Science advances 7: PubMed33674306

2021

Engineered glycomaterial implants orchestrate large-scale functional repair of brain tissue chronically after severe traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Charles-Francois V Latchoumane,Martha I Betancur,Gregory A Simchick,Min Kyoung Sun,Rameen Forghani,Christopher E Lenear,Aws Ahmed,Ramya Mohankumar,Nivedha Balaji,Hannah D Mason,Stephanie A Archer-Hartmann,Parastoo Azadi,Philip V Holmes,Qun Zhao,Ravi V Bellamkonda,Lohitash Karumbaiah

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOX1 antibody

Western blot - Anti-SOX1 antibody (AB87775)

Western blot - Anti-SOX1 antibody (AB87775)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

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Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (32)

Product promise