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Rabbit Recombinant Monoclonal SOX1 antibody. Carrier free. Suitable for mIHC, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (AB263870), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (AB263870), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (AB263870), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (AB263870), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (AB263870), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPWBIHC-FrIHC-P
Mouse
Tested
Not recommended
Not recommended
Tested
Tested
Rat
Expected
Not recommended
Not recommended
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Alternative names

Sox-1, Sox1, Sox-1, Transcription factor SOX-1

Recommended products

Rabbit Recombinant Monoclonal SOX1 antibody. Carrier free. Suitable for mIHC, IHC-Fr, IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR23041-60

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab263870 is the carrier-free version of Anti-SOX1 antibody [EPR23041-60] ab242125.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/2000 dilution (0.293 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on the stem cells in rat cerebellum is observed. The section was incubated with Anti-SOX1 antibody [EPR23041-60] ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/100 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on rat cerebellum (PMID: 24676388) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/2000 dilution (0.293 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: No staining on the mouse testis.

    The section was incubated with Anti-SOX1 antibody [EPR23041-60] ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/2000 dilution (0.293 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on the stem cells in mouse hippocampus is observed (PMID: 22992951). The section was incubated with Anti-SOX1 antibody [EPR23041-60] ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/100 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on mouse cerebellum (PMID: 24676388) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/2000 dilution (0.293 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on the stem cells in mouse cerebellum is observed (PMID: 24676388). The section was incubated with Anti-SOX1 antibody [EPR23041-60] ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

  • Multiplex immunohistochemistry - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX1 antibody [EPR23041-60] ab242125).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebellum tissue labelling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1:2000 (0.29 ug/ml) dilution, RoR alpha/RORA with Anti-ROR alpha/RORA antibody [EPR23719-18] ab256799 at 1:800 (0.63 ug/ml) dilution and SOX10 with ab200078 at 1:5000 (0.2 ug/ml) followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-SOX1 (magenta; Opal™690), anti-RORA (green; Opal™520) and anti-SOX10 (gray; Opal™570) on mouse cerebellum.

    Panel B: anti-SOX1 staining Bergmann glia in mouse cerebellum.

    Panel C: anti-RORA staining Pukinje cell and molecular layers in mouse cerebellum.

    Panel D: anti-SOX10 staining glial cells in mouse cerebellum.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-SOX1 antibody [EPR23041-60] ab242125, Anti-ROR alpha/RORA antibody [EPR23719-18] ab256799 and ab200078 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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