Rabbit Recombinant Monoclonal SOX1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 20 publications.
Images
![Western blot - Anti-SOX1 antibody [EPR4766] (AB109290), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-ab109290--western-blot-img178695.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOX1 antibody [EPR4766] (AB109290)")
![Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (AB109290), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-ab109290--flow-cytometry-intracellular-img174661.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (AB109290)")
![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (AB109290), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-ab109290--immunocytochemistry-immunofluorescence-img165716.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (AB109290)")
Publications
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- PloS one 18:e02880322023Effects of Rhazya Stricta plant organic extracts on human induced pluripotent stem cells derived neural stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdullah Othman Alawad,Faisal Sultan Alagrafi,Ahmed Jaman Alfahad,Hala Abdulrahman Alamari,Fatimah Othman Alghamdi,Hussam Mokhtar Fallatah,Alhassan Hamood Aodah,Sultan Suleiman Alyousef,Muhammed Adnan Bakhrebah,Ibrahim Oqla Alanazi,Mohannad Mokhtar FallatahPubMed 37478090
- Cell death discovery 9:1942023SOX1 acts as a tumor hypnotist rendering nasopharyngeal carcinoma cells refractory to chemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin-Xing Lei,Shu-Lan Wang,Ying Xia,Min Yan,Bin He,Bo Wang,Zi-Jie Long,Quentin LiuPubMed 37369660
- BMC biology 20:2192022Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaopeng Shen,Meng Li,Chunguang Wang,Zhongxian Liu,Kun Wu,Ao Wang,Chao Bi,Shan Lu,Hongan Long,Guoping ZhuPubMed 36199093
- The Journal of biological chemistry 298:1021062022Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Zhang,Yang Liu,Lihua Shi,Lei Fang,Liyang Xu,Ying CaoPubMed 35671824
- Cell death discovery 8:2562022CircFBXW7 inhibits the tumorigenesis of T-cell acute lymphoblastic leukemia through modulating miR-494-3p/SOX1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Luo,Jun-Jun Li,Feng Wen,Yi-Xiong Cao,Ze-Yu Luo,Xing-Xing LongPubMed 35538053
- Stem cell research 61:1027582022Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei-Ciao Tang,Marie V Roche,Se Young Um,Nicholas C Gosstola,Min Young Kim,Byung Yoon Choi,Derek M Dykxhoorn,Xue Zhong LiuPubMed 35364395
- Cancer cell international 21:6562021MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDa Wang,Fei Xiong,Guanhua Wu,Wenzheng Liu,Bing Wang,Yongjun ChenPubMed 34876142
- The Journal of biological chemistry 297:1012752021Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Chen,Min Zhang,Lei Fang,Xiaoli Yang,Ning Cao,Liyang Xu,Lihua Shi,Ying CaoPubMed 34619150
- Frontiers in cell and developmental biology 9:7143832021Suppression of Cell Tumorigenicity by Non-neural Pro-differentiation Factors via Inhibition of Neural Property in Tumorigenic Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Yang,Ning Cao,Lu Chen,Lin Liu,Min Zhang,Ying CaoPubMed 34595169
- Parkinson's disease 2021:66767092021Effect of NTN and Lmx1 on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Zhang,Bo Yang,Lilin Luo,Linhui Li,Xuantao Yang,Juanjuan Zhang,Yuxin Xie,Wanpu Wang,Shuaiyao LuPubMed 34373779
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Expected | Expected |
| Rat | Not recommended | Tested | Expected | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Alternative names
Transcription factor SOX-1, SOX1
Recommended products
Rabbit Recombinant Monoclonal SOX1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 20 publications.
Alternative names
Transcription factor SOX-1, SOX1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR4766
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SOX1 antibody [EPR4766] (ab109290), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-ab109290--western-blot-img178695.jpg "Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)")
Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)
All lanes: Western blot - Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution
Lane 1: NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2: Mouse brain lysate
Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4: Rat cerebral cortex lysate
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-ab109290--flow-cytometry-intracellular-img174661.jpg "Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290)")
Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290)
Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX1 with Purified ab109290 at 1:100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-ab109290--immunocytochemistry-immunofluorescence-img165716.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)")
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)
Immunocytochemistry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX1 with Purified ab109290 at 1:500 dilution (2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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