AB109290

Anti-SOX1 antibody [EPR4766]

Name: Anti-SOX1 antibody [EPR4766] (ab109290) | Abcam
Brand: Abcam Limited
SKU: AB109290
Price: 340 USD
Availability: InStock
Rating: 2 (4 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(4 Reviews)

(23 Publications)

Anti-SOX1 antibody [EPR4766] (ab109290) is a rabbit monoclonal antibody detecting SOX1 in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

Transcription factor SOX-1, SOX1

3 Images

Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (AB109290)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (AB109290)

Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX1 with Purified ab109290 at 1 : 100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (AB109290)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (AB109290)

Immunocytochemistry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX1 with Purified ab109290 at 1 : 500 dilution (2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Unknown

Western blot - Anti-SOX1 antibody [EPR4766] (AB109290)

All lanes:

Western blot - Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution

Lane 1:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

Rat cerebral cortex lysate at 20 µg

Lane 5:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 39 kDa

false

Carrier free

Anti-SOX1 antibody [EPR4766] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SOX1 antibody [EPR4766]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-SOX1 antibody [EPR4766]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4766

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-SOX1 antibody [EPR4766] (ab109290) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of SOX1?
Anti-SOX1 [EPR4766] (ab109290) specifically detects a band for SOX1 (UniProt: O00570) at a molecular weight of 39kDa.

Trusted by the scientific community
Anti-SOX1 [EPR4766] (ab109290) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4766] also available for your convenience: ab109290, Alexa Fluor® 488 - ab202338, Alexa Fluor® 647 - ab202339, Alexa Fluor® 594 - ab202514, Carrier free - ab227118

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

See full target information SOX1

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Acta naturae 16:62-72 PubMed39877013

2025

Embryonic Stem Cell Differentiation to Definitive Endoderm As a Model of Heterogeneity Onset During Germ Layer Specification.

Applications

Unspecified application

Species

Unspecified reactive species

M N Gordeev,A S Zinovyeva,E E Petrenko,E V Lomert,N D Aksenov,A N Tomilin,E I Bakhmet

Translational oncology 49:102104 PubMed39197387

2024

The role of deubiquitinase USP2 in driving bladder cancer progression by stabilizing EZH2 to epigenetically silence SOX1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fanghua Xu,Xiangda Xu,Huanhuan Deng,Zhaojun Yu,Jianbiao Huang,Leihong Deng,Haichao Chao

Cancer biology & therapy 25:2304161 PubMed38226837

2024

Hypoxia promotes non-small cell lung cancer cell stemness, migration, and invasion via promoting glycolysis by lactylation of SOX9.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yan,Yue Teng,Xiaoyou Li,Yuejiao Zhong,Chunyi Li,Feng Yan,Xia He

PloS one 18:e0288032 PubMed37478090

2023

Effects of Rhazya Stricta plant organic extracts on human induced pluripotent stem cells derived neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Abdullah Othman Alawad,Faisal Sultan Alagrafi,Ahmed Jaman Alfahad,Hala Abdulrahman Alamari,Fatimah Othman Alghamdi,Hussam Mokhtar Fallatah,Alhassan Hamood Aodah,Sultan Suleiman Alyousef,Muhammed Adnan Bakhrebah,Ibrahim Oqla Alanazi,Mohannad Mokhtar Fallatah

Cell death discovery 9:194 PubMed37369660

2023

SOX1 acts as a tumor hypnotist rendering nasopharyngeal carcinoma cells refractory to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Xing Lei,Shu-Lan Wang,Ying Xia,Min Yan,Bin He,Bo Wang,Zi-Jie Long,Quentin Liu

BMC biology 20:219 PubMed36199093

2022

Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Shen,Meng Li,Chunguang Wang,Zhongxian Liu,Kun Wu,Ao Wang,Chao Bi,Shan Lu,Hongan Long,Guoping Zhu

The Journal of biological chemistry 298:102106 PubMed35671824

2022

Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Yang Liu,Lihua Shi,Lei Fang,Liyang Xu,Ying Cao

Cell death discovery 8:256 PubMed35538053

2022

CircFBXW7 inhibits the tumorigenesis of T-cell acute lymphoblastic leukemia through modulating miR-494-3p/SOX1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Luo,Jun-Jun Li,Feng Wen,Yi-Xiong Cao,Ze-Yu Luo,Xing-Xing Long

Stem cell research 61:102758 PubMed35364395

2022

Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Ciao Tang,Marie V Roche,Se Young Um,Nicholas C Gosstola,Min Young Kim,Byung Yoon Choi,Derek M Dykxhoorn,Xue Zhong Liu

Cancer cell international 21:656 PubMed34876142

2021

MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Da Wang,Fei Xiong,Guanhua Wu,Wenzheng Liu,Bing Wang,Yongjun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOX1 antibody [EPR4766]

Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (AB109290)

Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (AB109290)

Western blot - Anti-SOX1 antibody [EPR4766] (AB109290)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (23)

Product promise