Rabbit Recombinant Monoclonal SOX1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Images
![Western blot - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (AB227118), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-bsa-and-azide-free-ab227118--western-blot-img178695.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (AB227118)")
![Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (AB227118), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-bsa-and-azide-free-ab227118--flow-cytometry-intracellular-img174661.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (AB227118)")
![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (AB227118), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-bsa-and-azide-free-ab227118--immunocytochemistry-immunofluorescence-img165716.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (AB227118)")
Publications
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- Development (Cambridge, England) 145:2018Genetic screening and multipotency in rhesus monkey haploid neural progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaisong Wang et. al.PubMed 29784672
- PloS one 9:e918522014Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells.
- Neuroscience letters 548:44-92013Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Expected | Expected |
| Rat | Not recommended | Tested | Expected | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
3 products for Alternative Version
Target data
Function
Transcriptional activator. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Alternative names
Transcription factor SOX-1, SOX1
Recommended products
Rabbit Recombinant Monoclonal SOX1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR4766
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab227118 is the carrier-free version of Anti-SOX1 antibody [EPR4766] ab109290.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-bsa-and-azide-free-ab227118--western-blot-img178695.jpg "Western blot - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)")
Western blot - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
All lanes: Western blot - Anti-SOX1 antibody [EPR4766] (Anti-SOX1 antibody [EPR4766] ab109290) at 1/1000 dilution
Lane 1: NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2: Mouse brain lysate
Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4: Rat cerebral cortex lysate
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-bsa-and-azide-free-ab227118--flow-cytometry-intracellular-img174661.jpg "Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)")
Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
This data was developed using ab227118, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX1 with Purified ab227118 at 1:100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118), expandable thumbnail](https://content.abcam.com/products/images/sox1-antibody-epr4766-bsa-and-azide-free-ab227118--immunocytochemistry-immunofluorescence-img165716.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)")
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
This data was developed using ab227118, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX1 with Purified ab227118 at 1:500 dilution (2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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