Anti-SOX10 antibody [EPR4007-104] (ab180862) is a rabbit monoclonal antibody detecting SOX10 in IHC-P, IHC-Fr, mIHC. Suitable for Human, Mouse, Rat.
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44581.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)")
![Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-frozen-sections-img14017.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)")
![Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-frozen-sections-img22318.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19628.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102575.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)")
Publications
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- Glia 71:1333-13452023Deficient deposition of new myelin impairs adult optic nerve function in a murine model of diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoqian Wu,Xianjun Chen,Bin Yu,Jieqiong Zhang,Xingmei Gu,Wei Liu,Feng Mei,Jian Ye,Lan XiaoPubMed 36661098
- Theranostics 12:5470-54872022Dedifferentiated Schwann cell-derived TGF-β3 is essential for the neural system to promote wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Yi Ou,Poh-Ching Tan,Yun Xie,Kai Liu,Yi-Ming Gao,Xiao-Sheng Yang,Shuang-Bai Zhou,Qing-Feng LiPubMed 35910794
- Nature communications 13:37322022Inactivation of the Hippo tumor suppressor pathway promotes melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarc A Vittoria,Nathan Kingston,Kristyna Kotynkova,Eric Xia,Rui Hong,Lee Huang,Shayna McDonald,Andrew Tilston-Lunel,Revati Darp,Joshua D Campbell,Deborah Lang,Xiaowei Xu,Craig J Ceol,Xaralabos Varelas,Neil J GanemPubMed 35768444
- Frontiers in neuroscience 15:6598532021DNA Hypermethylation Induced by L-Methionine Leads to Oligodendroglial and Myelin Deficits and Schizophrenia-Like Behaviors in Adolescent Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianjun Chen,Nan-Xin Huang,Yong-Jie Cheng,Qi-Yan Cai,Yan-Ping Tian,Xing-Shu Chen,Lan XiaoPubMed 33958986
- JCI insight 5:2020Schwannoma development is mediated by Hippo pathway dysregulation and modified by RAS/MAPK signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiguo Chen et. al.PubMed 32960816
- Cancer cell 36:302-318.e72019Single-Cell Transcriptomics in Medulloblastoma Reveals Tumor-Initiating Progenitors and Oncogenic Cascades during Tumorigenesis and Relapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiguo Zhang,Xuelian He,Xuezhao Liu,Feng Zhang,L Frank Huang,Andrew S Potter,Lingli Xu,Wenhao Zhou,Tao Zheng,Zaili Luo,Kalen P Berry,Allison Pribnow,Stephanie M Smith,Christine Fuller,Blaise V Jones,Maryam Fouladi,Rachid Drissi,Zeng-Jie Yang,W Clay Gustafson,Marc Remke,Scott L Pomeroy,Emily J Girard,James M Olson,A Sorana Morrissy,Maria C Vladoiu,Jiao Zhang,Weidong Tian,Mei Xin,Michael D Taylor,S Steven Potter,Martine F Roussel,William A Weiss,Q Richard LuPubMed 31474569
- PloS one 14:e02165272019The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank L Rice et. al.PubMed 31107888
- Journal of neuroscience research 97:828-8452019Xeno-free culture for generation of forebrain oligodendrocyte precursor cells from human pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulius Hermanto,Takakuni Maki,Yasushi Takagi,Susumu Miyamoto,Jun TakahashiPubMed 30891830
- Developmental cell 45:753-768.e82018Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuntao Zhao,Chen Dong,Magali Frah,Yaqi Deng,Corentine Marie,Feng Zhang,Lingli Xu,Zhixing Ma,Xinran Dong,Yifeng Lin,Scott Koenig,Brahim Nait-Oumesmar,Donna M Martin,Laiman N Wu,Mei Xin,Wenhao Zhou,Carlos Parras,Q Richard LuPubMed 29920279
- Cancer cell 33:292-308.e72018Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLai Man Natalie Wu,Yaqi Deng,Jincheng Wang,Chuntao Zhao,Jiajia Wang,Rohit Rao,Lingli Xu,Wenhao Zhou,Kwangmin Choi,Tilat A Rizvi,Marc Remke,Joshua B Rubin,Randy L Johnson,Thomas J Carroll,Anat O Stemmer-Rachamimov,Jianqiang Wu,Yi Zheng,Mei Xin,Nancy Ratner,Q Richard LuPubMed 29438698
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | IHC-P | ICC/IF | IP | WB | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended | Not recommended | Expected |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Expected | Expected | Not recommended | Not recommended | Not recommended | Tested |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Rat | Dilution info 1/50 | Notes Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
Alternative names
Transcription factor SOX-10, SOX10
Recommended products
Anti-SOX10 antibody [EPR4007-104] (ab180862) is a rabbit monoclonal antibody detecting SOX10 in IHC-P, IHC-Fr, mIHC. Suitable for Human, Mouse, Rat.
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4007-104
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-SOX10 antibody [EPR4007-104] (ab180862) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.
Trusted by the scientific community
Anti-SOX10 [EPR4007-104] (ab180862) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.
Reviewed by scientists
Anti-SOX10 [EPR4007-104] (ab180862) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR4007-104] also available for your convenience:
ab180862, Carrier free - Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free ab220078, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-SOX10 antibody [EPR4007-104] ab311012, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-SOX10 antibody [EPR4007-104] ab311140, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-SOX10 antibody [EPR4007-104] ab311777, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-SOX10 antibody [EPR4007-104] ab313058, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-SOX10 antibody [EPR4007-104] ab313258, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-SOX10 antibody [EPR4007-104] ab321238
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44581.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemical analysis of paraffin-embedded Mouse breast tissue labeling SOX10 with ab180862 at 1/500 dilution (0.22 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse breast. The section was incubated with ab180862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-frozen-sections-img14017.jpg "Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
![Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-frozen-sections-img22318.jpg "Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19628.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling SOX10 with ab180862 at 1/500 dilution (0.22 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse cerebellum.The section was incubated with ab180862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102575.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue labeling SOX10 with ab180862 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103836.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71996.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--multiplex-immunohistochemistry-img434342.jpg "Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (ab180862)
This data was developed using the same antibody clone in a different buffer formulation (Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free ab220078).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebellum tissue labelling SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1:2000 (0.29 ug/ml) dilution, RoR alpha/RORA with Anti-ROR alpha/RORA antibody [EPR23719-18] ab256799 at 1:800 (0.63 ug/ml) dilution and SOX10 with ab200078 at 1:5000 (0.2 ug/ml) followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-SOX1 (magenta; Opal™690), anti-RORA (green; Opal™520) and anti-SOX10 (gray; Opal™570) on mouse cerebellum.
Panel B: anti-SOX1 staining Bergmann glia in mouse cerebellum.
Panel C: anti-RORA staining Pukinje cell and molecular layers in mouse cerebellum.
Panel D: anti-SOX10 staining glial cells in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).The section was incubated in three rounds of staining: in the order of Anti-SOX1 antibody [EPR23041-60] ab242125, Anti-ROR alpha/RORA antibody [EPR23719-18] ab256799 and ab200078 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103836.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img71996.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
![Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (ab180862), expandable thumbnail](https://content.abcam.com/products/images/sox10-antibody-epr4007-104-ab180862--multiplex-immunohistochemistry-img434342.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (ab180862)")
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