Anti-SOX10 antibody [EPR4007-104]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human breast tissue staining Hormone sensitive lipase with ab325336 at a 1/2000 (0.248 μg/ml) dilution, ab318972 anti-COL8A1 used at a 1/2000 (0.264 μg/ml) dilution, and ab180862 anti-SOX10 used at a 1/4000 (0.03 μg/ml) dilution.

Panel A : anti-Hormone sensitive lipase (green; Opal™520), anti-COL8A1 (magenta; Opal™690), anti-SOX10 (gray; Opal™570) on human breast.

Panel B : anti-Hormone sensitive lipase staining adipocytes in human breast.

Panel C : anti-COL8A1 staining basement membrane in human breast.

Panel D : anti-SOX10 staining nucleus in human breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325336, ab318972 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human breast tissue staining Hormone sensitive lipase with ab325336 at a 1/2000 (0.248 μg/ml) dilution, ab315240 anti-Perlecan used at a 1/2000 (0.254 μg/ml) dilution, and ab180862 anti-SOX10 used at a 1/4000 (0.03 μg/ml) dilution.

Panel A : anti-Hormone sensitive lipase (green; Opal™520), anti-Perlecan (magenta; Opal™690), anti-SOX10 (gray; Opal™570) on human breast.

Panel B : anti-Hormone sensitive lipase staining adipocytes in human breast.

Panel C : anti-Perlecan staining basement membrane and endothelium in human breast.

Panel D : anti-SOX10 staining nucleus in human breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325336, ab315240 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue labeling SOX10 with ab180862 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control : PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (AB180862)

This data was developed using the same antibody clone in a different buffer formulation (ab220078).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebellum tissue labelling SOX1 with ab242125 at 1 : 2000 (0.29 ug/ml) dilution, RoR alpha/RORA with ab256799 at 1 : 800 (0.63 ug/ml) dilution and SOX10 with ab200078 at 1 : 5000 (0.2 ug/ml) followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-SOX1 (magenta; Opal^™690), anti-RORA (green; Opal^™520) and anti-SOX10 (gray; Opal^™570) on mouse cerebellum.
Panel B : anti-SOX1 staining Bergmann glia in mouse cerebellum.
Panel C : anti-RORA staining Pukinje cell and molecular layers in mouse cerebellum.
Panel D : anti-SOX10 staining glial cells in mouse cerebellum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab242125, ab256799 and ab200078 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control : PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling SOX10 with ab180862 at 1/500 dilution (0.22 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum.The section was incubated with ab180862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining Hormone sensitive lipase/HSL with ab325336 at a 1/2000 (0.248 μg/ml) dilution, ab324191 anti-E Cadherin used at a 1/500 (2.088 μg/ml) dilution and ab180862 anti-SOX10 used at a 1/4000 (0.03 μg/ml) dilution.

Panel A : anti-Hormone sensitive lipase/HSL (green; Opal™520), anti-E Cadherin (magenta; Opal™690), anti-SOX10 (gray; Opal™570) on mouse breast.

Panel B : anti-Hormone sensitive lipase/HSL staining adipocytes in mouse breast.

Panel C : anti-E Cadherin staining epithelium in mouse breast.

Panel D : anti-SOX10 staining on nucleus in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325336, ab324191 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining PNPLA3 with ab324436 at a 1/200 dilution, ab324191 anti-E Cadherin used at 1/500 dilution and ab155279 anti-SOX10 used at a 1/4000 dilution.

Panel A : anti-PNPLA3 (green; Opal™520), anti-E Cadherin (magenta; Opal™690) and anti-SOX10 (gray; Opal™570) on mouse breast.

Panel B : anti-PNPLA3 staining white adipose tissue in mouse breast.

Panel C : anti-E Cadherin staining membrane of epithelium in mouse breast.

Panel D : anti-SOX10 staining nucleus of epithelium in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324436, ab324191 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (AB180862)

Immunohistochemical analysis of paraffin-embedded Mouse breast tissue labeling SOX10 with ab180862 at 1/500 dilution (0.22 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse breast. The section was incubated with ab180862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).