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Anti-SOX10 antibody [EPR4007] (ab155279) is a rabbit monoclonal antibody detecting SOX10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Clone EPR4007 is the most cited clone to SOX10

- Biophysical QC for unrivalled batch-batch consistency

- Over 90 publications


Images

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (AB155279), expandable thumbnail
  • Western blot - Anti-SOX10 antibody [EPR4007] (AB155279), expandable thumbnail
  • Western blot - Anti-SOX10 antibody [EPR4007] (AB155279), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (AB155279), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (AB155279), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/500
Notes

For unpurified use at 1/250 - 1/500.

Species
Human
Dilution info
1/500
Notes

For unpurified use at 1/250 - 1/500.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).

Alternative names

Recommended products

Anti-SOX10 antibody [EPR4007] (ab155279) is a rabbit monoclonal antibody detecting SOX10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Clone EPR4007 is the most cited clone to SOX10

- Biophysical QC for unrivalled batch-batch consistency

- Over 90 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4007
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-SOX10 antibody [EPR4007] (ab155279) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, WB in human, mouse, rat samples.
Anti-SOX10 antibody [EPR4007] (ab155279) specifically detects SOX10 (UniProt ID: P56693; Molecular weight: 50kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-SOX10 antibody [EPR4007] (ab155279) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-SOX10 antibody [EPR4007] (ab155279) has been cited over 93 times in peer reviewed journals and is trusted by the scientific community.
Anti-SOX10 antibody [EPR4007] (ab155279) has 7 independent reviews from customers.

Related Products
Antibody clone EPR4007 is also available pre-conjugated to a variety of labels for your convenience - PE, APC, Alkaline Phosphatase, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 750 (PE Anti-SOX10 antibody [EPR4007] ab305382, APC Anti-SOX10 antibody [EPR4007] ab305383, Alkaline Phosphatase Anti-SOX10 antibody [EPR4007] ab309014, Alexa Fluor® 488 Anti-SOX10 antibody [EPR4007] ab309657, Alexa Fluor® 647 Anti-SOX10 antibody [EPR4007] ab310021, Alexa Fluor® 594 Anti-SOX10 antibody [EPR4007] ab310390, Alexa Fluor® 555 Anti-SOX10 antibody [EPR4007] ab311916, Alexa Fluor® 568 Anti-SOX10 antibody [EPR4007] ab312387, Alexa Fluor® 750 Anti-SOX10 antibody [EPR4007] ab321140).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279)

    Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab155279 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

  • Western blot - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-SOX10 antibody [EPR4007] (ab155279) at 1/2000 dilution

    All lanes: A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    Observed band size: 56-75 kDa

    Exposure time: 3s

  • Western blot - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)

    The bands observed are consistent with what have been described in PMID: 21423190

    All lanes: Western blot - Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution

    Lane 1: Human brain lysates at 20 µg

    Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: Mouse brain lysates at 20 µg

    Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

    Lane 5: Rat brain lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 49 kDa

    Observed band size: 56-75 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (ab155279)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1:50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (ab155279)

    Intracellular Flow Cytometry analysis ofA-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Western blot - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)

    SOX10 western blot using anti-SOX10 antibody [EPR4007] ab155279. Publication image and figure legend from Miyamoto, Y., Torii, T., et al., 2016, Nat Commun, PubMed 27876794.


    ab155279 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155279 please see the product overview.

    Knockout of VCAM1 in oligodendrocytes decreases myelin marker protein expression in mice.(a,b) MBP-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Mbp-Cre) and control mouse spinal cord cross sections at postnatal day 7 were stained with an antibody against GFAP (green) or NeuN (red). Data are representative. Scale bar, 200 μm (a); 100 μm (b). (c) The intensity of GFAP staining per one square millimetre was semi-quantified at postnatal days 7, 14 and 21. Data were evaluated using Student’s t-test (non-significance; n=6 slices of two independent experiments). (d) The number of NeuN+ cells per field was counted. Data were evaluated using Student’s t-test (non-significance; n=5 slices of two independent experiments). (e) Spinal cord cross sections of VCAM1fl/fl; Mbp-Cre and control mice at postnatal day 7 were co-stained with an antibody against CC1 (red) and DAPI (blue). Data are representative. Scale bar, 100 μm. (f,g) Antibodies against CC1 (red) and Olig2 (green) were used for co-staining in spinal cord cross sections at postnatal days 7, 14, 21 and 28. Data are representative. Scale bar, 100 μm. The percentage of CC1+ cells among the Olig2+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00638 (P7), 0.000961 (P14), 0.00111 (P21) or 2.11E-09 (P28); n=6–10 slices of two independent experiments). (h,i) Corpus callosum sections of VCAM1fl/fl; Mbp-Cre and control mouse (h) or VCAM1fl/fl; Ng2-Cre and control mouse (i) at postnatal day 11 were co-stained with an anti-MBP antibody (red) and DAPI (blue). Data are representative of six slices of two independent experiments. Scale bar, 200 μm. (j) Tissue lysates from 7-day-old spinal cords or 11-day-old brains were immunoblotted with an antibody against MBP, CNPase, VCAM1, CD69, MAG, Olig2, Sox10, Ascl1, Nkx2.1 or actin. Data are representative of three experiments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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