Anti-SOX10 antibody [EPR4007] (ab155279) is a rabbit monoclonal antibody detecting SOX10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.
- Clone EPR4007 is the most cited clone to SOX10
- Biophysical QC for unrivalled batch-batch consistency
- Over 90 publications
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
WB | IHC-Fr | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Tested | Expected | Tested | Tested |
Mouse | Tested | Tested | Expected | Expected |
Rat | Tested | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500 | Notes For unpurified use at 1/250 - 1/500. |
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/250 - 1/500. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
Transcription factor SOX-10, SOX10
Anti-SOX10 antibody [EPR4007] (ab155279) is a rabbit monoclonal antibody detecting SOX10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.
- Clone EPR4007 is the most cited clone to SOX10
- Biophysical QC for unrivalled batch-batch consistency
- Over 90 publications
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab155279 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-SOX10 antibody [EPR4007] (ab155279) at 1/2000 dilution
All lanes: A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 56-75 kDa
Exposure time: 3s
The bands observed are consistent with what have been described in PMID: 21423190
All lanes: Western blot - Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution
Lane 1: Human brain lysates at 20 µg
Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 3: Mouse brain lysates at 20 µg
Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg
Lane 5: Rat brain lysates at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 56-75 kDa
Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1:50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Intracellular Flow Cytometry analysis ofA-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Image collected and cropped by CiteAb under a CC-BY license from the publication
SOX10 western blot using anti-SOX10 antibody [EPR4007] ab155279. Publication image and figure legend from Miyamoto, Y., Torii, T., et al., 2016, Nat Commun, PubMed 27876794.
ab155279 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155279 please see the product overview.
Knockout of VCAM1 in oligodendrocytes decreases myelin marker protein expression in mice.(a,b) MBP-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Mbp-Cre) and control mouse spinal cord cross sections at postnatal day 7 were stained with an antibody against GFAP (green) or NeuN (red). Data are representative. Scale bar, 200 μm (a); 100 μm (b). (c) The intensity of GFAP staining per one square millimetre was semi-quantified at postnatal days 7, 14 and 21. Data were evaluated using Student’s t-test (non-significance; n=6 slices of two independent experiments). (d) The number of NeuN+ cells per field was counted. Data were evaluated using Student’s t-test (non-significance; n=5 slices of two independent experiments). (e) Spinal cord cross sections of VCAM1fl/fl; Mbp-Cre and control mice at postnatal day 7 were co-stained with an antibody against CC1 (red) and DAPI (blue). Data are representative. Scale bar, 100 μm. (f,g) Antibodies against CC1 (red) and Olig2 (green) were used for co-staining in spinal cord cross sections at postnatal days 7, 14, 21 and 28. Data are representative. Scale bar, 100 μm. The percentage of CC1+ cells among the Olig2+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00638 (P7), 0.000961 (P14), 0.00111 (P21) or 2.11E-09 (P28); n=6–10 slices of two independent experiments). (h,i) Corpus callosum sections of VCAM1fl/fl; Mbp-Cre and control mouse (h) or VCAM1fl/fl; Ng2-Cre and control mouse (i) at postnatal day 11 were co-stained with an anti-MBP antibody (red) and DAPI (blue). Data are representative of six slices of two independent experiments. Scale bar, 200 μm. (j) Tissue lysates from 7-day-old spinal cords or 11-day-old brains were immunoblotted with an antibody against MBP, CNPase, VCAM1, CD69, MAG, Olig2, Sox10, Ascl1, Nkx2.1 or actin. Data are representative of three experiments.
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