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Rabbit Recombinant Monoclonal SOX10 antibody. Suitable for WB, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 93 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/500

Notes

For unpurified use at 1/250 - 1/500.

Species

Human

Dilution info

1/500

Notes

For unpurified use at 1/250 - 1/500.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal SOX10 antibody. Suitable for WB, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 93 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4007

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279)

    Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab155279 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

  • Western blot - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-SOX10 antibody [EPR4007] (AB155279) at 1/2000 dilution

    All lanes: A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 49 kDa

    Observed band size: 56-75 kDa

    Exposure time: 3s

  • Western blot - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)

    The bands observed are consistent with what have been described in PMID: 21423190

    All lanes: Western blot - Anti-SOX10 antibody [EPR4007] (AB155279) at 1/1000 dilution

    Lane 1: Human brain lysates at 20 µg

    Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: Mouse brain lysates at 20 µg

    Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

    Lane 5: Rat brain lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 49 kDa

    Observed band size: 56-75 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (ab155279)

    Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1:50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (ab155279), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (ab155279)

    Intracellular Flow Cytometry analysis ofA-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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