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Anti-SOX10 antibody [SOX10/991] (ab218522) is a mouse monoclonal antibody that is used to detect SOX10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Recombinant format for unrivalled batch-batch consistency

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (AB218522), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SOX10/991] (AB218522), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SOX10/991] (AB218522), expandable thumbnail
  • Immunoprecipitation - Anti-SOX10 antibody [SOX10/991] (AB218522), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (AB218522), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human SOX10 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link P56693

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Predicted
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

7 products for Alternative Product

Target data

Function

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).

Alternative names

Transcription factor SOX-10, SOX10

Recommended products

Anti-SOX10 antibody [SOX10/991] (ab218522) is a mouse monoclonal antibody that is used to detect SOX10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Recombinant format for unrivalled batch-batch consistency

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human SOX10 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link P56693
Clone number
SOX10/991
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-SOX10 antibody [SOX10/991] (ab218522) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of SOX10?


Anti-SOX10 [SOX10/991] (ab218522) specifically detects a band for SOX10 (UniProt: P56693) at a molecular weight of 49kDa.

Other related products


We have a range of other formats of antibody clone [SOX10/991] also available for your convenience:
Anti-SOX10 antibody [EPR4007-104] ab180862, Carrier free - Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free ab220078, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-SOX10 antibody [EPR4007-104] ab311012, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-SOX10 antibody [EPR4007-104] ab311140, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-SOX10 antibody [EPR4007-104] ab311777, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-SOX10 antibody [EPR4007-104] ab313058, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-SOX10 antibody [EPR4007-104] ab313258, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-SOX10 antibody [EPR4007-104] ab321238, Carrier free - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free ab212843



What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human breast tissue using mouse Anti-SOX10 antibody

    Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab218522 at a 1/1000 dilution followed by a ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Nuclear staining in human breast.
    The section was incubated with ab218522 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Hematoxylin was used as a counter stain.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 Flow Cytometry (Intracellular) staining using mouse Anti-SOX10 antibody

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MCF-7(Human breast adenocarcinoma epithelial cell, Left) / A375 (Human malignant melanoma epithelial cell, Right) cells labelling SOX10 with ab218522 at 1/1000 dilution (0.1µg)/Red followed by a Goat Anti-Mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at a 1/2000 dilution compared with a Mouse monoclonal IgG (Black) isotype control and unlabelled control Cell without incubation with primary antibody and secondary antibody / Blue.

    Negative control: MCF7.(PMID: 23799842)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-SOX10 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labelling SOX10 with ab218522 at 1/50 dilution followed by secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (green).

    Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (red).

    Confocal image showing nuclear staining in A375 cells.
    Negative control: MCF7 (PMID: 23799842).
    Nuclear DNA was labelled with DAPI (shown in blue).

  • Immunoprecipitation - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Immunoprecipitation - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate with ab218522 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab218522 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-SOX10 antibody [SOX10/991] (ab218522) at 1/30 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate (Input) at 10 µg

    Lane 2: A375 whole cell lysate (+)

    Lane 3: Mouse IgG2b instead of ab218522 in A375 whole cell lysate (-)

    Secondary

    All lanes: Immunoprecipitation - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution

    Observed band size: 56-75 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat cerebrum tissue using mouse Anti-SOX10 antibody

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue using ab218522 at a 1/2000 dilution followed by a ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Nuclear staining in rat cerebrum.
    The section was incubated with ab218522 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Hematoxylin was used as a counter stain.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Western blot - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Western blot - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 Western blot staining using mouse Anti-SOX10 antibody

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.
    Negative control: MCF7 (PMID: 23799842).
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27115022, 29315345 ).

    All lanes: Western blot - Anti-SOX10 antibody [SOX10/991] (ab218522) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 56-75 kDa

    Exposure time: 26s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (ab218522), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] (ab218522)

    SOX10 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum tissue using mouse Anti-SOX10 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue using ab218522 at a 1/2000 dilution followed by a ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Nuclear staining in mouse cerebrum.
    The section was incubated with ab218522 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument
    Hematoxylin was used as a counter stain.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

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