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Rabbit Recombinant Monoclonal SOX10 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

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Images

Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (AB227684), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (AB227684), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684), expandable thumbnail
  • Western blot - Anti-SOX10 antibody [SP275] (AB227684), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info
1/400
Notes

-

Tested
Tested

Species

Human

Dilution info
1/400
Notes

-

Tested
Tested

Species

Human

Dilution info
1/100
Notes

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed:21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).

Alternative names

Transcription factor SOX-10, SOX10

Recommended products

Rabbit Recombinant Monoclonal SOX10 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP275

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (ab227684)

    Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1:400 dilution (1.02 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling SOX10 with ab227684 at 1/100 dilution (4.09 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (ab227684)

    Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1:50 (8.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Western blot - Anti-SOX10 antibody [SP275] (ab227684)

    All lanes: Western blot - Anti-SOX10 antibody [SP275] (ab227684) at 1/400 dilution

    All lanes: A-375 (human malignant melanoma cell line) cell lysate

    Predicted band size: 49 kDa

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (ab227684)

    Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells labeling SOX10 with ab227684 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)

    Formalin-fixed, paraffin-embedded human breast tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com