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AB227684

Anti-SOX10 antibody [SP275]

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(3 Publications)

Rabbit Recombinant Monoclonal SOX10 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Transcription factor SOX-10, SOX10

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (AB227684)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (AB227684)

Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1 : 50 (8.1 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (AB227684)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (AB227684)

Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells labeling SOX10 with ab227684 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684)

Formalin-fixed, paraffin-embedded human breast tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (AB227684)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling SOX10 with ab227684 at 1/100 dilution (4.09 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (AB227684)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP275] (AB227684)

Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1 : 400 dilution (1.02 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Western blot - Anti-SOX10 antibody [SP275] (AB227684)
  • WB

Supplier Data

Western blot - Anti-SOX10 antibody [SP275] (AB227684)

All lanes:

Western blot - Anti-SOX10 antibody [SP275] (ab227684) at 1/400 dilution

All lanes:

A-375 (human malignant melanoma cell line) cell lysate

Predicted band size: 49 kDa

false

  • Carrier free

    Anti-SOX10 antibody [SP275] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP275

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays a central role in developing and mature glia (By similarity). Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination (By similarity). Once induced, MYRF cooperates with SOX10 to implement the myelination program (By similarity). Transcriptional activator of MITF, acting synergistically with PAX3 (PubMed : 21965087). Transcriptional activator of MBP, via binding to the gene promoter (By similarity).
See full target information SOX10
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:3129781 PubMed35585935

2022

Curcumin Inhibits the Growth and Metastasis of Melanoma via miR-222-3p/SOX10/Notch Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Youqun Tang,Yanming Cao

Cancer management and research 13:7225-7234 PubMed34557039

2021

SOX10 Knockdown Inhibits Melanoma Cell Proliferation via Notch Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Youqun Tang,Yanming Cao

Pathology, research and practice 215:983-987 PubMed30739805

2019

Sinonasal glomangiopericytoma: A clinicopathologic study.

Applications

Unspecified application

Species

Unspecified reactive species

Farres Obeidin,Lawrence J Jennings,Borislav A Alexiev
View all publications
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Product promise

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