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AB134107

Anti-SOX11 antibody [EPR8192]

4

(1 Review)

|

(13 Publications)

Rabbit Recombinant Monoclonal SOX11 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

Transcription factor SOX-11, SOX11

3 Images
Immunoprecipitation - Anti-SOX11 antibody [EPR8192] (AB134107)
  • IP

Lab

Immunoprecipitation - Anti-SOX11 antibody [EPR8192] (AB134107)

SOX11 was immunoprecipitated from 0.35 mg Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 μg with 134107 at 1/40 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 μg

Lane 2 : ab134107 IP in Y79 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab134107 in Y79 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SOX11 antibody [EPR8192] (ab134107)

Predicted band size: 47 kDa

Observed band size: 60 kDa

false

Western blot - Anti-SOX11 antibody [EPR8192] (AB134107)
  • WB

Unknown

Western blot - Anti-SOX11 antibody [EPR8192] (AB134107)

All lanes:

Western blot - Anti-SOX11 antibody [EPR8192] (ab134107) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Y-79 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

Observed band size: 60 kDa

false

OI-RD Scanning - Anti-SOX11 antibody [EPR8192] (AB134107)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SOX11 antibody [EPR8192] (AB134107)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-SOX11 antibody [EPR8192] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8192

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that acts as a transcriptional activator (PubMed : 24886874, PubMed : 26543203). Binds cooperatively with POU3F2/BRN2 or POU3F1/OCT6 to gene promoters, which enhances transcriptional activation (By similarity). Acts as a transcriptional activator of TEAD2 by binding to its gene promoter and first intron (By similarity). Plays a redundant role with SOX4 and SOX12 in cell survival of developing tissues such as the neural tube, branchial arches and somites, thereby contributing to organogenesis (By similarity).
See full target information SOX11
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410808 PubMed39555675

2024

Reconstructing the Stem Leydig Cell Niche via the Testicular Extracellular Matrix for the Treatment of Testicular Leydig Cell Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ani Chi,Chao Yang,Jie Liu,Zhichen Zhai,Xuetao Shi

Nature cell biology 26:2020-2034 PubMed39528700

2024

Specialized post-arterial capillaries facilitate adult bone remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Vishal Mohanakrishnan,Kishor K Sivaraj,Hyun-Woo Jeong,Esther Bovay,Backialakshmi Dharmalingam,M Gabriele Bixel,Van Vuong Dinh,Milena Petkova,Isidora Paredes Ugarte,Yi-Tong Kuo,Malarvizhi Gurusamy,Brian Raftrey,Nelson Tsz Long Chu,Soumyashree Das,Pamela E Rios Coronado,Martin Stehling,Lars Sävendahl,Andrei S Chagin,Taija Mäkinen,Kristy Red-Horse,Ralf H Adams

Nature communications 15:2120 PubMed38459012

2024

Stem Leydig cells support macrophage immunological homeostasis through mitochondrial transfer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ani Chi,Bicheng Yang,Hao Dai,Xinyu Li,Jiahui Mo,Yong Gao,Zhihong Chen,Xin Feng,Menghui Ma,Yanqing Li,Chao Yang,Jie Liu,Hanchao Liu,Zhenqing Wang,Feng Gao,Yan Liao,Xuetao Shi,Chunhua Deng,Min Zhang

Nature communications 13:7335 PubMed36443325

2022

Targeting APLN/APJ restores blood-testis barrier and improves spermatogenesis in murine and human diabetic models.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Song,Xinyan Yang,Geng An,Xinyu Xia,Jiexiang Zhao,Xiaoheng Xu,Cong Wan,Tianyuan Liu,Yi Zheng,Shaofang Ren,Mei Wang,Gang Chang,Shane J F Cronin,Josef M Penninger,Tao Jing,Xianghong Ou,Shuan Rao,Zhaoting Liu,Xiao-Yang Zhao

Development (Cambridge, England) 148: PubMed34184026

2021

scRNA sequencing uncovers a TCF4-dependent transcription factor network regulating commissure development in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Theres Wittmann,Sayako Katada,Elisabeth Sock,Philipp Kirchner,Arif B Ekici,Michael Wegner,Kinichi Nakashima,Dieter Chichung Lie,André Reis

Disease models & mechanisms 14: PubMed33969421

2021

Sox11 regulates mammary tumour-initiating and metastatic capacity in Brca1-deficient mouse mammary tumour cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siu Man Tsang,Hyojin Kim,Erik Oliemuller,Richard Newman,Naa-Anyima Boateng,Naomi Guppy,Beatrice A Howard

Oncology reports 44:1511-1523 PubMed32945462

2020

lncRNA PCNAP1 predicts poor prognosis in breast cancer and promotes cancer metastasis via miR‑340‑5p‑dependent upregulation of SOX4.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Yaning He,Yingbo Shao,Qi Chen,Hui Liu

BMC cancer 19:619 PubMed31234811

2019

Master regulator analysis of paragangliomas carrying SDHx, VHL, or MAML3 genetic alterations.

Applications

Unspecified application

Species

Unspecified reactive species

John A Smestad,L James Maher

Cell reports 25:2689-2703.e3 PubMed30517858

2018

Single-Cell RNA-Seq of Mouse Olfactory Bulb Reveals Cellular Heterogeneity and Activity-Dependent Molecular Census of Adult-Born Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Burak Tepe,Matthew C Hill,Brandon T Pekarek,Patrick J Hunt,Thomas J Martin,James F Martin,Benjamin R Arenkiel

Scientific reports 8:16196 PubMed30385877

2018

Phosphorylation of the neurogenic transcription factor SOX11 on serine 133 modulates neuronal morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Elli-Anna Balta,Iris Schäffner,Marie-Theres Wittmann,Elisabeth Sock,Felix von Zweydorf,Julia von Wittgenstein,Kathrin Steib,Birgit Heim,Elisabeth Kremmer,Benjamin Martin Häberle,Marius Ueffing,Dieter Chichung Lie,Christian Johannes Gloeckner
View all publications
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Product promise

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