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Rabbit Recombinant Monoclonal SOX11 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Transcription factor that acts as a transcriptional activator (PubMed:24886874). Binds cooperatively with POU3F2/BRN2 or POU3F1/OCT6 to gene promoters, which enhances transcriptional activation (By similarity). Acts as a transcriptional activator of TEAD2 by binding to its gene promoter and first intron (By similarity). Plays a redundant role with SOX4 and SOX12 in cell survival of developing tissues such as the neural tube, branchial arches and somites, thereby contributing to organogenesis (By similarity).

Alternative names

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Rabbit Recombinant Monoclonal SOX11 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Carrier free

Yes

Clone number

EPR8192

Purification technique

Affinity purification Protein A

Dissociation constant

3.7 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248687 is the carrier-free version of ab134107.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-SOX11 antibody [EPR8192] - BSA and Azide free (ab248687), expandable thumbnail

    Immunoprecipitation - Anti-SOX11 antibody [EPR8192] - BSA and Azide free (ab248687)

    This data was developed using ab134107, the same antibody clone in a different buffer formulation.SOX11 was immunoprecipitated from 0.35 mg Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 µg with ab134107 at 1/40 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Y79 (Human retinoblastoma retinoblastoma) whole cell lysate 10 µg

    Lane 2: abab134107 IP in Y79 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab134107 in Y79 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-SOX11 antibody [EPR8192] (AB134107)

    Predicted band size: 47 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-SOX11 antibody [EPR8192] - BSA and Azide free (ab248687), expandable thumbnail

    Western blot - Anti-SOX11 antibody [EPR8192] - BSA and Azide free (ab248687)

    This data was developed using ab134107, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SOX11 antibody [EPR8192] (AB134107) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Y-79 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 47 kDa

    Observed band size: 60 kDa

  • OI-RD Scanning - Anti-SOX11 antibody [EPR8192] - BSA and Azide free (ab248687), expandable thumbnail

    OI-RD Scanning - Anti-SOX11 antibody [EPR8192] - BSA and Azide free (ab248687)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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