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Mouse Monoclonal SOX2 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 54 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Sox2.


Images

Flow Cytometry - Anti-SOX2 antibody [20G5] (AB171380), expandable thumbnail
  • Western blot - Anti-SOX2 antibody [20G5] (AB171380), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (AB171380), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (AB171380), expandable thumbnail
  • Flow Cytometry - Anti-SOX2 antibody [20G5] (AB171380), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Mouse Sox2. Database link P48432

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Expected
Tested
Tested
Tested
Tested
Mouse
Predicted
Expected
Expected
Tested
Expected
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Predicted

Expected
Expected

Species

Human

Dilution info

5 µg/mg of lysate

Notes

-

Predicted
Predicted

Species

Mouse, Sheep, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Sheep, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Sheep, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Predicted
Predicted

Species

Sheep, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Sheep, Chicken

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

Alternative names

Recommended products

Mouse Monoclonal SOX2 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 54 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Sox2.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Mouse Sox2. Database link P48432
Clone number

20G5

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail

    Flow Cytometry - Anti-SOX2 antibody [20G5] (ab171380)

    Flow cytometry analysis of H9 embryonic stem cells labeling SOX2 (blue histogram), using ab171380 at a 1/100 dilution, or a mouse IgG (green histogram) at a 1/100 dilution. A fluorescein-conjugated secondary antibody at a 1/200 dilution was used for the analysis.

  • Western blot - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail

    Western blot - Anti-SOX2 antibody [20G5] (ab171380)

    All lanes: Western blot - Anti-SOX2 antibody [20G5] (ab171380) at 1/500 dilution

    Lane 1: NCCIT (Human pluripotent embryonic carcinoma cell line) cell lysate at 25 µg

    Lane 2: NTERRA cell lysate at 25 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 25 µg

    Secondary

    All lanes: mouse IgG-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail
    Image from Lu L. et al., Sci Rep. 2018 Aug 14;8(1):12083. Fig2c. doi: 10.1038/s41598-018-29441-3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (ab171380)

    REST expression in N-hREST mouse brains correlates with stemness in embryonic neural stem cells. Immunofluorescence analysis of E18.5 N-hREST and LSL-hRESTcontrol littermate mouse brains with antibodies against REST (using an antibody that preferentially recognizes hREST over mouse REST) and SOX2 (using ab171380).

    Mice were anesthetized and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Brain tissues were then dissected and fixed in 4% PFA overnight at 4 °C. Fixed brain tissues were processed for paraffin embedding and then cut into 5-μm sections.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (ab171380)

    Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated human lung squamous carcinoma (right) compared with a negative control without primary antibody (left).

    To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with a SOX2 monoclonal antibody (ab171380) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Flow Cytometry - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail

    Flow Cytometry - Anti-SOX2 antibody [20G5] (ab171380)

    Flow cytometry analysis of HEL 11.4 induced IPS cells labeling SOX2 (blue histogram), using ab171380 at a 1/100 dilution, or a mouse IgG (green histogram) at a 1/100 dilution. A fluorescein-conjugated secondary antibody at a 1/200 dilution was used for the analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [20G5] (ab171380)

    Immunofluorescence analysis of formaldehyde-fixed HEL 11.4 induced IPS cells, labeling SOX2 using ab171380 (left panel) at a 1/200 dilution overnight. DAPI was used to stain the cell nuclei (central panel). Slides were washed with PBS and incubated with a fluorescein-conjugated secondary antibody at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (ab171380), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (ab171380)

    Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated mouse esophagus tissue (right) compared with a negative control without primary antibody (left).

    To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with a SOX2 monoclonal antibody (ab171380) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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Product protocols

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