Anti-SOX2 antibody [20G5]

• WB

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Western blot - Anti-SOX2 antibody [20G5] (AB171380)

All lanes:

Western blot - Anti-SOX2 antibody [20G5] (ab171380) at 1/500 dilution

Lane 1:

NCCIT (Human pluripotent embryonic carcinoma cell line) cell lysate at 25 µg

Lane 2:

NTERRA cell lysate at 25 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 25 µg

Secondary

All lanes:

mouse IgG-HRP at 1/10000 dilution

Predicted band size: 34 kDa

true

• Flow Cyt

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Flow Cytometry - Anti-SOX2 antibody [20G5] (AB171380)

Flow cytometry analysis of HEL 11.4 induced IPS cells labeling SOX2 (blue histogram), using ab171380 at a 1/100 dilution, or a mouse IgG (green histogram) at a 1/100 dilution. A fluorescein-conjugated secondary antibody at a 1/200 dilution was used for the analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [20G5] (AB171380)

Immunofluorescence analysis of formaldehyde-fixed HEL 11.4 induced IPS cells, labeling SOX2 using ab171380 (left panel) at a 1/200 dilution overnight. DAPI was used to stain the cell nuclei (central panel). Slides were washed with PBS and incubated with a fluorescein-conjugated secondary antibody at a 1/100 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (AB171380)

Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated human lung squamous carcinoma (right) compared with a negative control without primary antibody (left).

To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H₂O₂-methanol for 15 minutes at room temperature, washed with ddH₂O and PBS, and then probed with a SOX2 monoclonal antibody (ab171380) diluted by 3% BSA-PBS at a dilution of 1 : 200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-SOX2 antibody [20G5] (AB171380)

Flow cytometry analysis of H9 embryonic stem cells labeling SOX2 (blue histogram), using ab171380 at a 1/100 dilution, or a mouse IgG (green histogram) at a 1/100 dilution. A fluorescein-conjugated secondary antibody at a 1/200 dilution was used for the analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (AB171380)

Immunohistochemistry analysis of SOX2 showing staining in the nucleus of paraffin-treated mouse esophagus tissue (right) compared with a negative control without primary antibody (left).

To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H₂O₂-methanol for 15 minutes at room temperature, washed with ddH₂O and PBS, and then probed with a SOX2 monoclonal antibody (ab171380) diluted by 3% BSA-PBS at a dilution of 1 : 20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [20G5] (AB171380)

REST expression in N-hREST mouse brains correlates with stemness in embryonic neural stem cells. Immunofluorescence analysis of E18.5 N-hREST and LSL-hRESTcontrol littermate mouse brains with antibodies against REST (using an antibody that preferentially recognizes hREST over mouse REST) and SOX2 (using ab171380).

Mice were anesthetized and perfused with phosphate-buffered saline followed by 4% paraformaldehyde (PFA). Brain tissues were then dissected and fixed in 4% PFA overnight at 4 °C. Fixed brain tissues were processed for paraffin embedding and then cut into 5-μm sections.

Image from Lu L. et al., Sci Rep. 2018 Aug 14;8(1):12083. Fig2c. doi: 10.1038/s41598-018-29441-3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.