Mouse Monoclonal SOX2 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Mouse, Human, Apteronotus leptorhynchus samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img165672.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112)")
![Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--flow-cytometry-intracellular-img78217.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112)")
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img123831.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112)")
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img115208.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112)")
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img67049.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (AB264112)")
Publications
Filter by
- CNS neuroscience & therapeutics 29:202-2152022Intermittent hypoxic conditioning restores neurological dysfunction of mice induced by long-term hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaifen Li,Yuying Guan,Yakun Gu,Mengyuan Guo,Wei Ma,Qianqian Shao,Jia Liu,Xunming JiPubMed 36401601
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
| Apteronotus leptorhynchus | Predicted | Expected | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.10000-1.00000 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Rat, Sheep, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey, Apteronotus leptorhynchus | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Apteronotus leptorhynchus | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Rat, Sheep, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Rat, Sheep, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey, Apteronotus leptorhynchus | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
7 products for Alternative Product
Target data
Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).
Alternative names
Transcription factor SOX-2, SOX2
Recommended products
Mouse Monoclonal SOX2 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Mouse, Human, Apteronotus leptorhynchus samples. Cited in 1 publication.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
9-9-3
- Purification technique
Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab264112 is the carrier-free version of Anti-SOX2 antibody [9-9-3] ab79351.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img165672.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)")
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
This data was developed using the same antibody clone in a different buffer formulation without PBS and sodium azide (Anti-SOX2 antibody [9-9-3] ab79351)
Anti-SOX2 antibody [9-9-3] ab79351 staining SOX2 in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-SOX2 antibody [9-9-3] ab79351 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
![Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--flow-cytometry-intracellular-img78217.jpg "Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)")
Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
This image was produced using the same antibody clone, but in a different formulation, Anti-SOX2 antibody [9-9-3] ab79351.
Overlay histogram showing F9 cells stained with Anti-SOX2 antibody [9-9-3] ab79351 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-SOX2 antibody [9-9-3] ab79351, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed F9 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img123831.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)")
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
This image was produced using the same antibody clone, but in a different formulation, Anti-SOX2 antibody [9-9-3] ab79351.
Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX2 with Anti-SOX2 antibody [9-9-3] ab79351 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on NCCIT cells. Counterstained with Anti-beta IV Tubulin antibody [EPR16775] ab179504 anti-Tubulin (Rabbit mAb, 1/1000), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
The negative controls are as follows:
-ve control 1: Anti-SOX2 antibody [9-9-3] ab79351 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img115208.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)")
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
This image was produced using the same antibody clone, but in a different formulation, Anti-SOX2 antibody [9-9-3] ab79351.
Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma) cells labeling SOX2 with Anti-SOX2 antibody [9-9-3] ab79351 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on F9 cells. Counterstained with Anti-beta IV Tubulin antibody [EPR16775] ab179504 anti-Tubulin (Rabbit mAb, 1/1000), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
The negative controls are as follows:
-ve control 1: Anti-SOX2 antibody [9-9-3] ab79351 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112), expandable thumbnail](https://content.abcam.com/products/images/sox2-antibody-9-9-3-bsa-and-azide-free-ab264112--immunocytochemistry-immunofluorescence-img67049.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)")
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
This image was produced using the same antibody clone, but in a different formulation, Anti-SOX2 antibody [9-9-3] ab79351.
Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) (Negative control) cells labeling SOX2 with Anti-SOX2 antibody [9-9-3] ab79351 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing no staining on NIH/3T3 cells. Counterstained with Anti-beta IV Tubulin antibody [EPR16775] ab179504 anti-Tubulin (Rabbit mAb, 1/1000), Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
The negative controls are as follows:
-ve control 1: Anti-SOX2 antibody [9-9-3] ab79351 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 at 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com