Rabbit Polyclonal SOX2 antibody. Suitable for IP, IHC-P, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOX2.
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- International journal of molecular sciences 24:2023The W101C Mutation Induces Slower Pacing by Constitutively Active GIRK Channels in hiPSC-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Kayser,Sven Dittmann,Tomo Šarić,Giulia Mearini,Arie O Verkerk,Eric Schulze-BahrPubMed 37894977
- The EMBO journal 42:e1103842023The transcriptional co-activator Yap1 promotes adult hippocampal neural stem cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqiang Fan,Jerónimo Jurado-Arjona,Gregorio Alanis-Lobato,Sophie Péron,Christian Berger,Miguel A Andrade-Navarro,Sven Falk,Benedikt BerningerPubMed 37083045
- Biochemistry and biophysics reports 32:1013422022Saikosaponin D improves chemosensitivity of glioblastoma by reducing the its stemness maintenance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Liang,Jing Sun,Aibin Liu,Lei Chen,Xiaofang Ma,Xiaozhi Liu,Chunyan ZhangPubMed 36186734
- Advanced therapeutics 5:2022An induced pluripotent stem cell-derived NMJ platform for study of the NGLY1-Congenital Disorder of Deglycosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrevor Sasserath,Ashley L Robertson,Roxana Mendez,Tristan T Hays,Ethan Smith,Helena Cooper,Nesar Akanda,John W Rumsey,Xiufang Guo,Atena Farkhondeh,Manisha Pradhan,Karsten Baumgaertel,Matthew Might,Steven Rodems,Wei Zheng,James J HickmanPubMed 36589922
- International journal of molecular sciences 23:2022Effects of Long-Term Temozolomide Treatment on Glioblastoma and Astrocytoma WHO Grade 4 Stem-like Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas Feldheim,Almuth F Kessler,Julia J Feldheim,Ellina Schulz,David Wend,Lazaros Lazaridis,Christoph Kleinschnitz,Martin Glas,Ralf-Ingo Ernestus,Sebastian Brandner,Camelia M Monoranu,Mario Löhr,Carsten HagemannPubMed 35563629
- Frontiers in oncology 12:8052902022Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeitao Zhang,Lu Kong,Hongbin Zhu,Decong Sun,Quanli Han,Bin Yan,Zhi Cui,Weiwei Zhang,Shurong Zhang,Xindan Kang,Guanghai Dai,Niansong Qian,Wenji YanPubMed 35299743
- STAR protocols 2:1009642021Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAida Menéndez-Méndez,Lucía Paniagua-Herranz,Luis A Olivos-Oré,Rosa Gómez-Villafuertes,Raquel Pérez-Sen,Esmerilda G Delicado,Antonio R Artalejo,Felipe OrtegaPubMed 34841278
- International journal of molecular sciences 22:2021Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Ceriani,Kathleen E WhitlockPubMed 34072957
- The journal of gene medicine 23:e33252021CircVAPA exerts oncogenic property in non-small cell lung cancer by the miR-876-5p/WNT5A axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhao,Qiangsheng Dai,Xiaohong Fu,Qianqi Chen,Yueqiang Tang,Xiaoping Gao,Qiming ZhouPubMed 33619796
- Stem cell research & therapy 12:2142021Transplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYajie Gong,Haoyun Duan,Xin Wang,Can Zhao,Wenjing Li,Chunxiao Dong,Zongyi Li,Qingjun ZhouPubMed 33781330
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SOX2. The exact immunogen used to generate this antibody is proprietary information. Database link P48431
Consider this alternative
Reactivity data
Select an application| IP | IHC-P | Flow Cyt | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Expected | Expected | Tested |
| Cat | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes or Tris-EDTA buffer (pH8.0) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Monkey | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/500 | Notes - |
Species Human | Dilution info 1/50 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Monkey | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed:18035408). This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Transcription factor SOX-2, SOX2
Recommended products
Rabbit Polyclonal SOX2 antibody. Suitable for IP, IHC-P, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 29 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOX2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SOX2. The exact immunogen used to generate this antibody is proprietary information. Database link P48431
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
19 product images
Flow Cytometry - Anti-SOX2 antibody (ab137385)
Flow cytometric analysis of human embryonic stem cells, labelling SOX2 with ab137385 at 1/100 dilution (purple) compared to a rabbit IgG control (black).
Western blot - Anti-SOX2 antibody (ab137385)
10% SDS-PAGE gel
All lanes: Western blot - Anti-SOX2 antibody (ab137385) at 1/1000 dilution
All lanes: Human ESC whole cell lysate at 20 µg
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab137385)
Formalin-fixed, paraffin-embedded Cal27 xenograft tissue stained for SOX2 with ab137385 at a 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)
Human embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.
Western blot - Anti-SOX2 antibody (ab137385)
12% SDS-PAGE gel
All lanes: Western blot - Anti-SOX2 antibody (ab137385) at 1/1000 dilution
All lanes: Mouse ESC whole cell lysate at 20 µg
Predicted band size: 24 kDa, 32 kDa, 34 kDa
Western blot - Anti-SOX2 antibody (ab137385)
10% SDS-PAGE gel
All lanes: Western blot - Anti-SOX2 antibody (ab137385) at 1/3000 dilution
All lanes: NT2D1 whole cell lysate at 30 µg
Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)
Mouse embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)
Paraformaldehyde-fixed A549 cells stained for SOX2 (green) using ab137385 (1/200 dilution) in ICC/IF. Lower panel is merged with a DNA probe.
Immunohistochemistry (Frozen sections) - Anti-SOX2 antibody (ab137385)
Immunohistochemical analysis of Frozen sectioned adult mouse retina staining SOX2 protein with ab137385 at a dilution of 1/250 (Green). Red: Protein kinase C alpha staining. The nuclear staining is DAPI (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)
Immunofluorescence analysis of 4% paraformaldehyde fixed NT2D1 cells labelling SOX2 protein using ab137385 at a 1/500 dilution at RT for 15 min (Green). Phalloidin, a cytoskeleton marker was used to counterstain phalloidin diluted at 1/200 dilution(Red).
Scale bar = 10 µm.
Immunohistochemistry (Frozen sections) - Anti-SOX2 antibody (ab137385)
Immunohistochemical analysis of Frozen sectioned E13.5 rat brain staining SOX2 protein with ab137385 at a dilution of 1/250 (Green). Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1/250 dilution.The nuclear staining is DAPI (Blue).
Immunoprecipitation - Anti-SOX2 antibody (ab137385)
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of ab137385.
Western blot analysis was performed using ab137385.
An anti-Rabbit IgG was used as a secondary reagent.All lanes: Immunoprecipitation - Anti-SOX2 antibody (ab137385)
All lanes: NT2D1 whole cell extracts
SecondaryAll lanes: anti-Rabbit IgG
Predicted band size: 34 kDa
Immunoprecipitation - Anti-SOX2 antibody (ab137385)
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of ab137385.
Western blot analysis was performed using ab137385 diluted at 1:500.
An anti-Rabbit IgG was used as a secondary reagent.All lanes: Immunoprecipitation - Anti-SOX2 antibody (ab137385) at 1/500 dilution
All lanes: NT2D1 whole cell extracts
SecondaryAll lanes: anti-Rabbit IgG
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab137385)
Paraffin-embedded rat brain stem tissue stained for SOX2 protein at nucleus in rat brain stem with ab137385 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab137385)
Paraffin-embedded mouse esophagus tissue stained for SOX2 protein at nucleus in mouse esophagus with ab137385 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab137385)
Paraffin-embedded mouse fore brain tissue stained for SOX2 protein at nucleus on mouse fore brain with ab137385 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab137385)
Paraffin-embedded mouse fetal brain tissue stained for SOX2 protein at nucleus in mouse fetal brain with ab137385 at a 1/200 dilution in immunohistochemical analysis. The signal was developed using goat anti-rabbit IgG antibody (Dylight488).
Blue: Nuclear staining with Hoechst 33342.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry (Frozen sections) - Anti-SOX2 antibody (ab137385)
Immunohistochemical analysis of Frozen-sectioned adult mouse hippocampus staining SOX2 protein with ab137385 at a dilution of 1/250 (Green). The nuclear staining is DAPI (Blue).
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-SOX2 antibody (ab137385)
SOX2 western blot using anti-SOX2 antibody ab137385. Publication image and figure legend from Lu, G., Li, Y., et al., 2018, J Exp Clin Cancer Res, PubMed 30482236.
ab137385 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137385 please see the product overview.
LncRNA LINC00511 contributes to the maintenance of breast cancer CSC characteristic. a LINC00511 expression in breast cancer cell lines MDA-MB-468, MDA-MB-231, MDA-MB-453, and MCF-7 and normal cell lines (MCF-10A). b, c Breast cancer cell MDA-MB-231, and MCF-7 LINC00511 expression. d Stem factor (Oct4, Nanog, SOX2) expression in MDA-MB-231 cells transfected with shRNA for LINC00511. e The stem factors (Oct4, Nanog, SOX2) expression in MCF-7 cells transfected with enhanced LINC00511 plasmids. f, g Mammosphere diameter and quantity when transfected with shRNA for LINC00511. h, i Mammosphere diameter and quantity when transfected with enhanced LINC00511 plasmids. ** indicates p value less than 0.01. * indicates p value less than 0.05
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