Anti-SOX2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Formalin-fixed, paraffin-embedded Cal27 xenograft tissue stained for SOX2 with ab137385 at a 1/100 dilution in immunohistochemical analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB137385)

Human embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.

• Flow Cyt

Unknown

Flow Cytometry - Anti-SOX2 antibody (AB137385)

Flow cytometric analysis of human embryonic stem cells, labelling SOX2 with ab137385 at 1/100 dilution (purple) compared to a rabbit IgG control (black).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Paraffin-embedded human cervical carcinoma tissue stained for SOX2 protein at nucleus in human cervical carcinoma with ab137385 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Paraffin-embedded human esophageal carcinoma tissue stained for SOX2 protein at nucleus with ab137385 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB137385)

Immunofluorescence analysis of 4% paraformaldehyde fixed NT2D1 cells labelling SOX2 protein using ab137385 at a 1/500 dilution at RT for 15 min (Green). Phalloidin, a cytoskeleton marker was used to counterstain phalloidin diluted at 1/200 dilution(Red).
Scale bar = 10 µm.

• IP

Supplier Data

Immunoprecipitation - Anti-SOX2 antibody (AB137385)

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of ab137385. Western blot analysis was performed using ab137385. An anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-SOX2 antibody (ab137385)

All lanes:

NT2D1 whole cell extracts

Secondary

All lanes:

anti-Rabbit IgG

Predicted band size: 34 kDa

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• IP

Supplier Data

Immunoprecipitation - Anti-SOX2 antibody (AB137385)

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of ab137385. Western blot analysis was performed using ab137385 diluted at 1 : 500. An anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-SOX2 antibody (ab137385) at 1/500 dilution

All lanes:

NT2D1 whole cell extracts

Secondary

All lanes:

anti-Rabbit IgG

Predicted band size: 34 kDa

false

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SOX2 antibody (AB137385)

Immunohistochemical analysis of Frozen sectioned adult mouse retina staining SOX2 protein with ab137385 at a dilution of 1/250 (Green). Red : Protein kinase C alpha staining. The nuclear staining is DAPI (Blue).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-SOX2 antibody (AB137385)

Immunohistochemical analysis of Frozen-sectioned adult mouse hippocampus staining SOX2 protein with ab137385 at a dilution of 1/250 (Green). The nuclear staining is DAPI (Blue).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-SOX2 antibody (AB137385)

Immunohistochemical analysis of Frozen sectioned E13.5 rat brain staining SOX2 protein with ab137385 at a dilution of 1/250 (Green). Red : beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1/250 dilution.The nuclear staining is DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Paraffin-embedded rat brain stem tissue stained for SOX2 protein at nucleus in rat brain stem with ab137385 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Paraffin-embedded mouse esophagus tissue stained for SOX2 protein at nucleus in mouse esophagus with ab137385 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Paraffin-embedded mouse fetal brain tissue stained for SOX2 protein at nucleus in mouse fetal brain with ab137385 at a 1/200 dilution in immunohistochemical analysis. The signal was developed using goat anti-rabbit IgG antibody (Dylight488).
Blue : Nuclear staining with Hoechst 33342.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB137385)

Mouse embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB137385)

Paraffin-embedded mouse fore brain tissue stained for SOX2 protein at nucleus on mouse fore brain with ab137385 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : (EDTA based, pH 8.0) buffer, 15min

• WB

Supplier Data

Western blot - Anti-SOX2 antibody (AB137385)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-SOX2 antibody (ab137385) at 1/2500 dilution

All lanes:

hESC Whole cell extracts at 30 µg

Predicted band size: 34 kDa

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• WB

Supplier Data

Western blot - Anti-SOX2 antibody (AB137385)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-SOX2 antibody (ab137385) at 1/15000 dilution

Lane 1:

Non-transfected NT2D1 cells at 30 µg

Lane 2:

Transfected NT2D1 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-SOX2 antibody (AB137385)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-SOX2 antibody (ab137385) at 1/2500 dilution

All lanes:

Mouse ESC lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34 kDa

false

• WB

CiteAb

Western blot - Anti-SOX2 antibody (AB137385)

SOX2 western blot using anti-SOX2 antibody ab137385. Publication image and figure legend from Lu, G., Li, Y., et al., 2018, J Exp Clin Cancer Res, PubMed 30482236.

ab137385 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137385 please see the product overview.

LncRNA LINC00511 contributes to the maintenance of breast cancer CSC characteristic. a LINC00511 expression in breast cancer cell lines MDA-MB-468, MDA-MB-231, MDA-MB-453, and MCF-7 and normal cell lines (MCF-10A). b, c Breast cancer cell MDA-MB-231, and MCF-7 LINC00511 expression. d Stem factor (Oct4, Nanog, SOX2) expression in MDA-MB-231 cells transfected with shRNA for LINC00511. e The stem factors (Oct4, Nanog, SOX2) expression in MCF-7 cells transfected with enhanced LINC00511 plasmids. f, g Mammosphere diameter and quantity when transfected with shRNA for LINC00511. h, i Mammosphere diameter and quantity when transfected with enhanced LINC00511 plasmids. ** indicates p value less than 0.01. * indicates p value less than 0.05

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