Anti-SOX2 antibody (ab239218) is a goat polyclonal antibody that is used to detect SOX2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.
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- Frontiers in cell and developmental biology 11:12708922023Transcriptome analysis reveals genes associated with stem cell activation by physical exercise in the dentate gyrus of aged p16Ink4a knockout mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Micheli,Giorgio D'Andrea,Teresa Maria Creanza,Daniel Volpe,Nicola Ancona,Raffaella Scardigli,Felice TironePubMed 37928906
- Frontiers in cell and developmental biology 9:6966842021Transcriptome Analysis in a Mouse Model of Premature Aging of Dentate Gyrus: Rescue of Alpha-Synuclein Deficit by Virus-Driven Expression or by Running Restores the Defective Neurogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Micheli,Teresa Maria Creanza,Manuela Ceccarelli,Giorgio D'Andrea,Giacomo Giacovazzo,Nicola Ancona,Roberto Coccurello,Raffaella Scardigli,Felice TironePubMed 34485283
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SOX2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P48431
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Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-0.3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Chicken, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Chicken, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Chicken, Mouse | Dilution info - | Notes - |
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Target data
Function
The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed:18035408). This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Transcription factor SOX-2, SOX2
Recommended products
Anti-SOX2 antibody (ab239218) is a goat polyclonal antibody that is used to detect SOX2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SOX2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P48431
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-SOX2 antibody (ab239218) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab239218)
Immunofluorescence analysis of paraformaldehyde fixed U-251 MG (Human brain glioma cell line) cells, permeabilized with 0.15% Triton, labeling SOX2 (green) using ab239218. Primary incubation time 1 hour (10 μg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 μg/ml). Actin filaments were stained with phalloidin (red).
Negative control: Non-immunized goat IgG (10 μg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 μg/ml).
Western blot - Anti-SOX2 antibody (ab239218)
Detected by chemiluminescence.
All lanes: Western blot - Anti-SOX2 antibody (ab239218) at 0.3 µg/mL
Lane 1: MCF-7 cell lysates at 35 µg
Lane 2: HEK293 cell lysates at 35 µg
Lane 3: MCF7 nuclear cell lysates at 35 µg
Predicted band size: 34 kDa
Flow Cytometry (Intracellular) - Anti-SOX2 antibody (ab239218)
Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr using ab239218 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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