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Anti-SOX2 antibody ab97959 is a rabbit polyclonal antibody that is used in SOX2 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

Same trusted quality, new lower price


Images

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB97959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB97959), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB97959), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (AB97959), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Tested
Expected
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Horse
Predicted
Predicted
Predicted
Oncorhynchus mykiss
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Quail
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Spotted catshark
Predicted
Predicted
Predicted
Thornback ray
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody.

Species

Rat

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody.

Species

Human

Dilution info

1 µg/mL

Notes

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody.

Predicted
Predicted

Species

Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB.

Species

Rat

Dilution info

1 µg/mL

Notes

Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB.

Species

Human

Dilution info

1 µg/mL

Notes

Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB.

Predicted
Predicted

Species

Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark

Dilution info

-

Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

Target data

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

Alternative names

Recommended products

Anti-SOX2 antibody ab97959 is a rabbit polyclonal antibody that is used in SOX2 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.

Same trusted quality, new lower price

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959), expandable thumbnail
    Muratore CR et al. Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols. PLoS One 9:e105807 (2014). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)

    ICC/IF analysis of dissociated induced pluripotent stem cells from mouse embryonic fibroblasts stained for SOX-2 (Red) using ab97959. TOPRO (purple). Scale bar=100 μm

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)

    ab97959 staining SOX2 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)

    IHC image of SOX2 staining in Human brain glioma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97959, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)

    ab97959 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 0.1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)

    Cell line: NCCIT (human pluripotent embryonal carcinoma)

    Target AbID: ab97959 anti-Sox2, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used.

    Counterstain AbID: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (Rabbit mAb), 97959

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on NCCIT cells

    Target primary antibody dilution: 1:500

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:500 (ab97959)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)

  • Western blot - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Western blot - Anti-SOX2 antibody (ab97959)

    Green signal from target - ab97959 observed at 40 kDa
    Red signal from loading control Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (GAPDH) observed at 37 kDa

    All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1 µg

    Lane 1: NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 2: F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 4: C6 (Rat glial tumor cell line) Whole Cell Lysate at 10 µg

    Lane 5: Hippocampus (Rat) Tissue Lysate at 10 µg

    Lane 6: Spinal Cord (Rat) Tissue Lysate at 10 µg

    Secondary

    All lanes: Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    Performed under reducing conditions.

    Predicted band size: 34 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Western blot - Anti-SOX2 antibody (ab97959)

    Secondary antibody - goat anti-rabbit HRP preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080)

    All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1/1000 dilution

    All lanes: Western blot - Recombinant Human SOX2 protein (Recombinant Human SOX2 protein ab79950) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa

    Exposure time: 30s

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)

    Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)

    Target AbID: ab97959 anti-Sox2, used Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody

    Counterstain AbID: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (Rabbit mAb), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on NIH/3T3 cells

    Target primary antibody dilution: 1:500

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:500 (ab97959)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)

  • Western blot - Anti-SOX2 antibody (ab97959), expandable thumbnail

    Western blot - Anti-SOX2 antibody (ab97959)

    All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1 µg/mL

    Lane 1: IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate at 10 µg

    Lane 2: HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa

    Observed band size: 37 kDa, 39 kDa, 43 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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