Anti-SOX2 antibody ab97959 is a rabbit polyclonal antibody that is used in SOX2 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Same trusted quality, new lower price
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
ICC/IF | WB | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Tested |
Rat | Tested | Tested | Expected |
Chicken | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Horse | Predicted | Predicted | Predicted |
Oncorhynchus mykiss | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Quail | Predicted | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted | Predicted |
Sheep | Predicted | Predicted | Predicted |
Spotted catshark | Predicted | Predicted | Predicted |
Thornback ray | Predicted | Predicted | Predicted |
Xenopus laevis | Predicted | Predicted | Predicted |
Zebrafish | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
Species Rat | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
Species Human | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
Species Rat | Dilution info 1 µg/mL | Notes Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
Species Human | Dilution info 1 µg/mL | Notes Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
Species | Dilution info | Notes |
---|---|---|
Species Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark | Dilution info - | Notes - |
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Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).
Transcription factor SOX-2, SOX2
Anti-SOX2 antibody ab97959 is a rabbit polyclonal antibody that is used in SOX2 western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
Same trusted quality, new lower price
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
ICC/IF analysis of dissociated induced pluripotent stem cells from mouse embryonic fibroblasts stained for SOX-2 (Red) using ab97959. TOPRO (purple). Scale bar=100 μm
ab97959 staining SOX2 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
IHC image of SOX2 staining in Human brain glioma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97959, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab97959 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 0.1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Cell line: NCCIT (human pluripotent embryonal carcinoma)
Target AbID: ab97959 anti-Sox2, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used.
Counterstain AbID: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (Rabbit mAb), 97959
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NCCIT cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)
Green signal from target - ab97959 observed at 40 kDa
Red signal from loading control Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (GAPDH) observed at 37 kDa
All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1 µg
Lane 1: NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: C6 (Rat glial tumor cell line) Whole Cell Lysate at 10 µg
Lane 5: Hippocampus (Rat) Tissue Lysate at 10 µg
Lane 6: Spinal Cord (Rat) Tissue Lysate at 10 µg
All lanes: Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 40 kDa
Secondary antibody - goat anti-rabbit HRP preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080)
All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1/1000 dilution
All lanes: Western blot - Recombinant Human SOX2 protein (Recombinant Human SOX2 protein ab79950) at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 30s
Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)
Target AbID: ab97959 anti-Sox2, used Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody
Counterstain AbID: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (Rabbit mAb), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NIH/3T3 cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)
All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1 µg/mL
Lane 1: IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate at 10 µg
Lane 2: HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa, 39 kDa, 43 kDa
Exposure time: 3min
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