Anti-SOX2 antibody (ab97959) is a rabbit polyclonal antibody detecting SOX2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 730 publications
- Trusted since 2010
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- Molecular psychiatry 29:809-8192023ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiji Kawatani,Marie-Louise Holm,Skylar C Starling,Yuka A Martens,Jing Zhao,Wenyan Lu,Yingxue Ren,Zonghua Li,Peizhou Jiang,Yangying Jiang,Samantha K Baker,Ni Wang,Bhaskar Roy,Tammee M Parsons,Ralph B Perkerson,Hanmei Bao,Xianlin Han,Guojun Bu,Takahisa KanekiyoPubMed 38135757
- Cancers 15:2023Development of a Genetically Engineered Mouse Model Recapitulating LKB1 and PTEN Deficiency in Gastric Cancer Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuan-Te Fang,Hsin Hung,Nga Yin Sadonna Lau,Jou-Hsi Chi,Deng-Chyang Wu,Kuang-Hung ChengPubMed 38136437
- Cells 12:2023Human Embryonic Stem Cell-Derived Immature Midbrain Dopaminergic Neurons Transplanted in Parkinsonian Monkeys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdolfo López-Ornelas,Itzel Escobedo-Avila,Gabriel Ramírez-García,Rolando Lara-Rodarte,César Meléndez-Ramírez,Beetsi Urrieta-Chávez,Tonatiuh Barrios-García,Verónica A Cáceres-Chávez,Xóchitl Flores-Ponce,Francia Carmona,Carlos Alberto Reynoso,Carlos Aguilar,Nora E Kerik,Luisa Rocha,Leticia Verdugo-Díaz,Víctor Treviño,José Bargas,Verónica Ramos-Mejía,Juan Fernández-Ruiz,Aurelio Campos-Romo,Iván VelascoPubMed 38067166
- Nature communications 14:77172023Mouse models of pediatric high-grade gliomas with MYCN amplification reveal intratumoral heterogeneity and lineage signatures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie Schoof,Shweta Godbole,Thomas K Albert,Matthias Dottermusch,Carolin Walter,Annika Ballast,Nan Qin,Marlena Baca Olivera,Carolin Göbel,Sina Neyazi,Dörthe Holdhof,Catena Kresbach,Levke-Sophie Peter,Gefion Dorothea Epplen,Vanessa Thaden,Michael Spohn,Mirjam Blattner-Johnson,Franziska Modemann,Martin Mynarek,Stefan Rutkowski,Martin Sill,Julian Varghese,Ann-Kristin Afflerbach,Alicia Eckhardt,Daniel Münter,Archana Verma,Nina Struve,David T W Jones,Marc Remke,Julia E Neumann,Kornelius Kerl,Ulrich SchüllerPubMed 38001143
- Journal of neuroinflammation 20:2612023NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Chen,Xue-Yan Liu,Mou-Hui Lin,Yu-Xi Li,De-Zhi Kang,Zu-Cheng Ye,Qing-Song LinPubMed 37953259
- Scientific data 10:7552023Profiling mitochondria-polyribosome lncRNAs associated with pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhou,Hui Li,Tingge Sun,Xue Wen,Chao Niu,Min Li,Wei Li,Miguel A Esteban,Andrew R Hoffman,Ji-Fan Hu,Jiuwei CuiPubMed 37919270
- Frontiers in cell and developmental biology 11:12589932023Combined 3D bioprinting and tissue-specific ECM system reveals the influence of brain matrix on stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Zamponi,Peter A Mollica,Yara Khodour,Julie S Bjerring,Robert D Bruno,Patrick C SachsPubMed 37928905
- Development (Cambridge, England) 150:2023Caudal Fgfr1 disruption produces localised spinal mis-patterning and a terminal myelocystocele-like phenotype in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEirini Maniou,Faduma Farah,Abigail R Marshall,Zoe Crane-Smith,Andrea Krstevski,Athanasia Stathopoulou,Nicholas D E Greene,Andrew J Copp,Gabriel L GaleaPubMed 37756583
- The EMBO journal 42:e1133832023N4BP1 mediates RAM domain-dependent notch signaling turnover during neocortical development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihua Ma,Yi Zeng,Ming Wang,Wei Liu,Jiafeng Zhou,Chao Wu,Lin Hou,Bin Yin,Boqin Qiang,Pengcheng Shu,Xiaozhong PengPubMed 37807845
- Molecular psychiatry 28:3769-37812023Mitochondrial proteins encoded by the 22q11.2 neurodevelopmental locus regulate neural stem and progenitor cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilip D Campbell,Isaiah Lee,Summer Thyme,Michael GranatoPubMed 37794116
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Tested |
| Rat | Tested | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Oncorhynchus mykiss | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Quail | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
| Spotted catshark | Predicted | Predicted | Predicted |
| Thornback ray | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
Species Rat | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
Species Human | Dilution info 1 µg/mL | Notes We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
Species Rat | Dilution info 1 µg/mL | Notes Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
Species Human | Dilution info 1 µg/mL | Notes Recombinant Human SOX2 protein (ab80520) can be used as a positive control in WB. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Quail, Rhesus monkey, Oncorhynchus mykiss, Thornback ray, Spotted catshark | Dilution info - | Notes - |
Associated Products
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Target data
Function
The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed:18035408). This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Transcription factor SOX-2, SOX2
Recommended products
Anti-SOX2 antibody (ab97959) is a rabbit polyclonal antibody detecting SOX2 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 730 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-SOX2 antibody (ab97959) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-SOX2 antibody (ab97959) was first used in a scientific publication in 2009 and has been cited over 736 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-SOX2 antibody (ab97959) has high sensitivity and specificity.
Anti-SOX2 antibody (ab97959) has 72 independent reviews from customers.
Anti-SOX2 antibody (ab97959) specifically detects SOX2 (UniProt ID: P48431; Molecular weight: 35kDa) and is sold in 100 µg selling sizes.
SOX2 is a crucial transcription factor in neuro research, particularly for its role in maintaining neural stem cell pluripotency and regulating neural development. It is essential for the proliferation and differentiation of neural progenitor cells, influencing brain development and repair. Researchers use SOX2 to study neural stem cell behaviour, neurogenesis and the mechanisms underlying neurodevelopmental disorders, making it vital for understanding brain development and potential regenerative therapies. Additionally, SOX2 plays a significant role in gliomas, particularly glioblastoma (GBM), the most aggressive form of brain cancer. Elevated SOX2 levels are associated with poor prognosis and tumor progression in glioblastoma patients. Targeting SOX2 expression and function is being explored as a potential therapeutic strategy to combat gliomas and improve patient outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab97959)
IHC image of SOX2 staining in Human brain glioma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97959, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Muratore CR et al. Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols. PLoS One 9:e105807 (2014). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)
ICC/IF analysis of dissociated induced pluripotent stem cells from mouse embryonic fibroblasts stained for SOX-2 (Red) using ab97959. TOPRO (purple). Scale bar=100 μm
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)
ab97959 staining SOX2 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)
ab97959 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab97959 at 0.1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)
Cell line: NCCIT (human pluripotent embryonal carcinoma)
Target AbID: ab97959 anti-Sox2, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used.
Counterstain AbID: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (Rabbit mAb), 97959
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NCCIT cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)
Western blot - Anti-SOX2 antibody (ab97959)
Green signal from target - ab97959 observed at 40 kDa
Red signal from loading control Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (GAPDH) observed at 37 kDaAll lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1 µg
Lane 1: NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: C6 (Rat glial tumor cell line) Whole Cell Lysate at 10 µg
Lane 5: Hippocampus (Rat) Tissue Lysate at 10 µg
Lane 6: Spinal Cord (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 40 kDa
Western blot - Anti-SOX2 antibody (ab97959)
Secondary antibody - goat anti-rabbit HRP preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080)
All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1/1000 dilution
All lanes: Western blot - Recombinant Human SOX2 protein (Recombinant Human SOX2 protein ab79950) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Exposure time: 30s
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab97959)
Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)
Target AbID: ab97959 anti-Sox2, used Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody
Counterstain AbID: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (Rabbit mAb), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary
Fixative: 4% PFA
Permeabilisation: 0.1% Triton-X
Nuclear counter stain: DAPI
Comments: Confocal image showing negative staining on NIH/3T3 cells
Target primary antibody dilution: 1:500
Target secondary antibody dilution: 1:1000 (2ug/mL)
Counterstain primary antibody dilution: 1:1000 (1ug/mL)
Counterstain secondary antibody dilution: 1:1000 (2ug/mL)
Negative control 1 primary antibody dilution: 1:500 (ab97959)
Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291)
Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)
Western blot - Anti-SOX2 antibody (ab97959)
All lanes: Western blot - Anti-SOX2 antibody (ab97959) at 1 µg/mL
Lane 1: IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate at 10 µg
Lane 2: HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa, 39 kDa, 43 kDa
Exposure time: 3min
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