Anti-SOX2 antibody [EPR3131]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemical analysis of formalin fixed paraffin embedded human glioma labelling SOX2 with ab92494 at a concentration of 0.1µg/ml.

The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5. ab92494 anti-SOX2 was incubated at 37°C for 16min.

Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Negative control : Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified ab92494 shows negative staining.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (AB92494)

Confocal image showing nuclear staining on NCCIT cells

ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

Negative control 2 Ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.

• IHC - Wmt

AbReview34440****

IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (AB92494)

IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).

This image is courtesy of an anonymous Abreview.

• ICC

AbReview66851****

Immunocytochemistry - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunocytochemistry analysis of paraformaldehyde-fixed human Neuromesodermal Progenitors permeabilized with 0.5% Triton X-100 staining with ab92494 at 1/200 dilution. Secondary antibody was Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/1000 dilution. Samples were incubated with the primary antibody with Blocking buffer for 18 hours at 4°C. Blocking was done using 10% serum for 1 hour at 25°C. 10% FCS, 0.1% BSA in PBS was used for blocking.

This image is courtesy of an Abreview submitted by Antigoni Gogolou

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (AB92494)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.

• sELISA

Unknown

Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (AB92494)

Standard Curve for SOX2 (Analyte : SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2μg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5μg/ml.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (AB92494)

SOX2 immunostaining in sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) embryos.

At E13, strong SOX2 staining was seen in the lingual region of the epithelial dental lamina in all mice (E, G & H) except for the Usag-1^+/+/Runx2^-/- mice, in which SOX2 was found throughout the dental lamina (F). At E15, strong SOX2 staining was seen in the additional lingual bud in the Usag-1^+/+/Runx2^-/- mice (N).

Image from Togo Y et al., PLoS One. 2016;11(8):e0161067. Fig 6.; doi: 10.1371/journal.pone.0161067. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (AB92494)

Confocal image showing nuclear staining on F9 cells

ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000) ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

Negative control 2 Ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (AB92494)

Transient Wnt and FGF signalling induce dual fated mouse neuromesodermal progenitors.

Immunostaining of cells treated with FGF/Wnt revealed the coexpression of Brachyury with Sox2 (NMPs). In the absence of Wnt, NPCs express Sox2 but the expression of Brachyury is only evident in a very small proportion of cells.

Image from Gouti M et al., PLoS Biol. 2014;12(8):e1001937. Fig 2.; doi: 10.1371/journal.pbio.1001937. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (AB92494)

ab92494 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab92494 at 1/100 dilution and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (AB92494)

Confocal image showing negative staining on NIH/3T3 cells.

ab92494 staining SOX2 in the NIH/3T3 (mouse embryonic fibroblast cell line) (negative control) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), ab150120 Alexa Fluor^® 594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 : ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

Negative control 2 : ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.

• WB

Lab

Western blot - Anti-SOX2 antibody [EPR3131] (AB92494)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

All lanes:

NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

• WB

Lab

Western blot - Anti-SOX2 antibody [EPR3131] (AB92494)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

Lane 1:

NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

SK-OV-3 (human ovarian cancer cell line) whole cell lysate at 10 µg

Lane 4:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 10 µg

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 6:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 7:

Human breast cancer tissue lysate at 10 µg

Lane 8:

Human glioma lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-SOX2 antibody [EPR3131] (AB92494)

All lanes:

Western blot - Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution

Lane 1:

NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

Lane 2:

MCF-7 (human breast adenocarcinoma cell line) cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 34 kDa

false

• WB

Lab

Western blot - Anti-SOX2 antibody [EPR3131] (AB92494)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1500 dilution

All lanes:

F9 (mouse embryonic testicular cancer cell line) cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

• WB

Lab

Western blot - Anti-SOX2 antibody [EPR3131] (AB92494)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

Lane 1:

F9 (mouse embryonic testicular cancer cell line) whole cell lysate at 10 µg

Lane 2:

4T1 (mouse mammary gland carcinoma cell line) whole cell lysate at 10 µg

Lane 3:

Mouse hippocampus lysate at 10 µg

Lane 4:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 5:

Rat hippocampus lysate at 10 µg

Lane 6:

Rat spinal cord lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Exposure time: 3min

• IHC - Wmt

AbReview37352****

IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (AB92494)

IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection : DAB.

This image is courtesy of an Abreview submitted by Dr. Gillis.