Anti-SOX2 antibody [EPR3131] - BSA and Azide free

15 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494)
  

  Anti-SOX2 antibody [EPR3131] ab92494 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-SOX2 antibody [EPR3131] ab92494 at 1/100 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

  Mass Cytometry - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Imaging Mass Cytometry™ (IMC™) image of human glioblastoma brain cancer tissue stained with Anti-SOX2 antibody [EPR3131]. ab215970 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm's protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.
  

  Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified Anti-SOX2 antibody [EPR3131] ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

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  This image is courtesy of an anonymous customer review.

  IHC - Wholemount - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified Anti-SOX2 antibody [EPR3131] ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

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  Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Confocal image showing nuclear staining on NCCIT cells

  Anti-SOX2 antibody [EPR3131] ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1/1000) was used as the secondary antibody. Counterstained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (1/1000), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

  Negative control 1 Anti-SOX2 antibody [EPR3131] ab92494 was used as the primary antibody at 1/200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 was used as the secondary at 1/1000.

  Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary at 1/1000.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

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  This image is courtesy of a customer review submitted by Dr. Gillis.

  IHC - Wholemount - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection: DAB.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

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  Sandwich ELISA - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (Recombinant Human SOX2 protein ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (Anti-SOX2 antibody [EPR3131] ab92494) at 0.5µg/ml.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified Anti-SOX2 antibody [EPR3131] ab92494.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified Anti-SOX2 antibody [EPR3131] ab92494 shows negative staining.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494.
  
  
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified Anti-SOX2 antibody [EPR3131] ab92494.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] - BSA and Azide free (ab215970)

  Confocal image showing nuclear staining on F9 cells

  Anti-SOX2 antibody [EPR3131] ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (1/1000) was used as the secondary antibody. Counterstained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (1/1000), Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 AlexaFluor®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

  Negative control 1 Anti-SOX2 antibody [EPR3131] ab92494 was used as the primary antibody at 1/200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 was used as the secondary at 1/1000.

  Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary at 1/1000.<\p>

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SOX2 antibody [EPR3131] ab92494).