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Mouse Monoclonal SOX2 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein, Mouse, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SOX2 aa 150 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (AB218520), expandable thumbnail
  • Western blot - Anti-SOX2 antibody [SOX2/1791] (AB218520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (AB218520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (AB218520), expandable thumbnail
  • Western blot - Anti-SOX2 antibody [SOX2/1791] (AB218520), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human SOX2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48431

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBProtein ArrayIHC-P
Human
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Recombinant full length protein
Tested
Not recommended
Not recommended
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Recombinant full length protein

Dilution info

1.00000-2.00000 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1.00000-2.00000 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein, Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

Alternative names

Transcription factor SOX-2, SOX2

Recommended products

Mouse Monoclonal SOX2 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein, Mouse, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SOX2 aa 150 to C-terminus.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human SOX2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P48431
Clone number

SOX2/1791

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (ab218520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded human cervical carcinoma tissue labeling SOX2 with ab218520 at 1 μg/mL dilution, followed by conjugation to the secondary antibody.

  • Western blot - Anti-SOX2 antibody [SOX2/1791] (ab218520), expandable thumbnail

    Western blot - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    All lanes: Western blot - Anti-SOX2 antibody [SOX2/1791] (ab218520) at 2 µg/mL

    All lanes: A549 (human lung carcinoma cell line) whole cell lysate

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (ab218520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling SOX2 with ab218520. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (ab218520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    Immunohistochemical analysis of formalin-fixed and paraffin-embedded human lung carcinoma tissue labeling SOX2 with ab218520.

  • Western blot - Anti-SOX2 antibody [SOX2/1791] (ab218520), expandable thumbnail

    Western blot - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    Lanes 1 - 2: Western blot - Anti-SOX2 antibody [SOX2/1792] (Anti-SOX2 antibody [SOX2/1792] ab218521)

    Lanes 1 - 2: Western blot - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    Lane 1: Recombinant SOX2 protein fragment

    Lane 2: NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate

    Predicted band size: 34 kDa

  • Protein Array - Anti-SOX2 antibody [SOX2/1791] (ab218520), expandable thumbnail

    Protein Array - Anti-SOX2 antibody [SOX2/1791] (ab218520)

    ab218520 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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