Mouse Monoclonal SOX2 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein, Mouse, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SOX2 aa 150 to C-terminus.
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
WB | Protein Array | IHC-P | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Expected | Expected | Tested |
Recombinant full length protein | Tested | Not recommended | Not recommended |
Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant full length protein | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein, Recombinant full length protein - Human | Dilution info - | Notes - |
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The protein expressed by the gene SOX2 is critical for early embryogenesis and for the pluripotency of embryonic stem cells (PubMed:18035408). This supplementary information is collated from multiple sources and compiled automatically.
Transcription factor SOX-2, SOX2
Mouse Monoclonal SOX2 antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein, Mouse, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SOX2 aa 150 to C-terminus.
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human lungcarcinoma tissue labeling SOX2 with ab218521.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labeling SOX2 with ab218521 at 2 μg/mL (30 minutes at room temperature). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervical carcinoma tissue labeling SOX2 with ab218521 at 1 μg/ml.
Lanes 1 - 2: Western blot - Anti-SOX2 antibody [SOX2/1792] (ab218521)
Lanes 1 - 2: Western blot - Anti-SOX2 antibody [SOX2/1791] (Anti-SOX2 antibody [SOX2/1791] ab218520)
Lane 1: Recombinant SOX2 protein fragment
Lane 2: NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling SOX2 with ab218521 at 2 μg/mL (30 minutes at room temperature). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 45 minutes at 95°C followed by cooling at room temperature for 20 minutes.
ab218521 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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