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AB183606

Anti-SOX3 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal SOX3 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SOX3 aa 1-200.

View Alternative Names

Transcription factor SOX-3, SOX3

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-SOX3 antibody (AB183606)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOX3 antibody (AB183606)

Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (human glioblastoma astrocytoma) cells labeling SOX3 with ab183606 at 1/500 (Green, nuclear expression). The cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Beta tubulin 3 was counterstained with mouse monoclonal anti-Beta Tubulin 3 antibody (red).

Immunoprecipitation - Anti-SOX3 antibody (AB183606)
  • IP

Supplier Data

Immunoprecipitation - Anti-SOX3 antibody (AB183606)

ab183606 at 5μg immunoprecipitating SOX3 protein in NT2D1 (human embryonal carcinoma) whole cell lysate (lane 2).

Lane 1 : NT2D1 whole cell lysate

Lane 2 : ab183606 + NT2D1 whole cell lysate

Western blot was performed using ab183606 followed by anti-rabbit IgG as the secondary antibody.

All lanes:

Immunoprecipitation - Anti-SOX3 antibody (ab183606)

Predicted band size: 45 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (AB183606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (AB183606)

Immunohistochemical analysis of mouse brain tissue sections labeling SOX3 with ab183606 at 1/500. Sections were embedded in Paraffin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (AB183606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (AB183606)

Immunohistochemical analysis of rat brain tissue sections labeling SOX3 with ab183606 at 1/200. A goat anti-rabbit IgG was used as the secondary antibody. Sections were embedded in Paraffin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (AB183606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX3 antibody (AB183606)

Immunohistochemical analysis of mouse brain tissue sections labeling SOX3 with ab183606 at 1/500. Sections were embedded in Paraffin.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SOX3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P41225

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity).
See full target information SOX3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2304161 PubMed38226837

2024

Hypoxia promotes non-small cell lung cancer cell stemness, migration, and invasion via promoting glycolysis by lactylation of SOX9.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yan,Yue Teng,Xiaoyou Li,Yuejiao Zhong,Chunyi Li,Feng Yan,Xia He

Nature communications 13:2387 PubMed35501322

2022

Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Papes,Antonio P Camargo,Janaina S de Souza,Vinicius M A Carvalho,Ryan A Szeto,Erin LaMontagne,José R Teixeira,Simoni H Avansini,Sandra M Sánchez-Sánchez,Thiago S Nakahara,Carolina N Santo,Wei Wu,Hang Yao,Barbara M P Araújo,Paulo E N F Velho,Gabriel G Haddad,Alysson R Muotri

Frontiers in genetics 12:616220 PubMed33995475

2021

NEAT1 Overexpression Indicates a Poor Prognosis and Induces Chemotherapy Resistance via the miR-491-5p/ Signaling Pathway in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhuan Jia,Lan Wei,Zhengmao Zhang

International journal of oncology 52:1277-1284 PubMed29484385

2018

Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjie Guo,Jimin Yin,Mingjie Tang,Xingang Yu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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