Rabbit Recombinant Monoclonal SOX4 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.
Images
![Dot Blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (AB316851), expandable thumbnail](https://content.abcam.com/products/images/sox4-antibody-epr28132-51-bsa-and-azide-free-ab316851--dot-blot-img428241.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (AB316851)")
![Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (AB316851), expandable thumbnail](https://content.abcam.com/products/images/sox4-antibody-epr28132-51-bsa-and-azide-free-ab316851--western-blot-img428207.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (AB316851)")
![Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (AB316851), expandable thumbnail](https://content.abcam.com/products/images/sox4-antibody-epr28132-51-bsa-and-azide-free-ab316851--western-blot-img428208.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (AB316851)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IP | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif (PubMed:30661772). Required for IL17A-producing Vgamma2-positive gamma-delta T-cell maturation and development, via binding to regulator loci of RORC to modulate expression (By similarity). Involved in skeletal myoblast differentiation by promoting gene expression of CALD1 (PubMed:26291311).
Alternative names
Transcription factor SOX-4, SOX4
Recommended products
Rabbit Recombinant Monoclonal SOX4 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28132-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316851 is the carrirer-free version of Anti-SOX4 antibody [EPR28132-51] ab316850.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851), expandable thumbnail](https://content.abcam.com/products/images/sox4-antibody-epr28132-51-bsa-and-azide-free-ab316851--dot-blot-img428241.jpg "Dot Blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851)")
Dot Blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851)
SOX4 Dot Blot staining using rabbit Anti-SOX4 antibody
This data was developed using Anti-SOX4 antibody [EPR28132-51] ab316850, the same antibody clone in a different buffer formulation.
Dot blot analysis of SOX4 using Anti-SOX4 antibody [EPR28132-51] ab316850 at 1:1000 (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: His-tagged human SOX4 recombinant protein fragment
Lane 2: His-tagged human SOX5 recombinant protein fragment
Lane 3: His-tagged human SOX6 recombinant protein fragmentThis antibody does not cross-react with human SOX5 and SOX6.
All lanes: Dot Blot - Anti-SOX4 antibody [EPR28132-51] (Anti-SOX4 antibody [EPR28132-51] ab316850) at 1/1000 dilution
Lane 1: His-tagged human SOX4 recombinant protein fragment
Lane 2: His-tagged human SOX5 recombinant protein fragment
Lane 3: His-tagged human SOX6 recombinant protein fragment
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851), expandable thumbnail](https://content.abcam.com/products/images/sox4-antibody-epr28132-51-bsa-and-azide-free-ab316851--western-blot-img428207.jpg "Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851)")
Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851)
This data was developed using Anti-SOX4 antibody [EPR28132-51] ab316850, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 38240850).
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The identity of the lower MW bands at approximately 25kDa and 37 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2:70 seconds; Lane 3 : 136 seconds
All lanes: Western blot - Anti-SOX4 antibody [EPR28132-51] (Anti-SOX4 antibody [EPR28132-51] ab316850) at 1/1000 dilution
Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: Jurkat transfected with siRNA specifically targeting SOX4 whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 70 kDa, 36 kDa
![Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851), expandable thumbnail](https://content.abcam.com/products/images/sox4-antibody-epr28132-51-bsa-and-azide-free-ab316851--western-blot-img428208.jpg "Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851)")
Western blot - Anti-SOX4 antibody [EPR28132-51] - BSA and Azide free (ab316851)
This data was developed using Anti-SOX4 antibody [EPR28132-51] ab316850, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 38240850).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
All lanes: Western blot - Anti-SOX4 antibody [EPR28132-51] (Anti-SOX4 antibody [EPR28132-51] ab316850) at 1/1000 dilution
Lane 1: DLD-1 (human colorectal adenocarcinoma epithelial cell) nuclear fraction at 20 µg with 5% NFDM/TBST
Lane 2: DLD-1 cytoplasmic fraction at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 70 kDa, 36 kDa, 15 kDa
Exposure time: 59s
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