Anti-SOX5 antibody (ab94396) is a rabbit polyclonal antibody that is used to detect SOX5 in Western Blot, IP, IHC-P, IHC-Fr, ChIP. Suitable for Human, Mouse samples.
- Over 30 publications
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- Communications biology 7:102024STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXian Wang,Chao Han,Di Yang,Jian Zhou,Hui Dong,Zhiyuan Wei,Shuai Xu,Chen Xu,Yiwei Zhang,Yi Sun,Bing Ni,Sheng Guo,Jingbo Zhang,Tingting Zhao,Xiangmei Chen,Jie Luo,Yuzhang Wu,Yi TianPubMed 38172644
- Cells 12:2023Early Regional Patterning in the Human Prefrontal Cortex Revealed by Laminar Dynamics of Deep Projection Neuron Markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanja Kopić,Alisa Junaković,Iva Salamon,Mladen-Roko Rasin,Ivica Kostović,Željka KrsnikPubMed 36672166
- Brain structure & function 228:613-6332023Laminar dynamics of deep projection neurons and mode of subplate formation are hallmarks of histogenetic subdivisions of the human cingulate cortex before onset of arealization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlisa Junaković,Janja Kopić,Alvaro Duque,Pasko Rakic,Željka Krsnik,Ivica KostovićPubMed 36592215
- NPJ Regenerative medicine 7:692022Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hPSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei Shen,Lu Chen,Dahe Zhang,Simo Xia,Zhuman Lv,Duohong Zou,Zhiyuan Zhang,Chi Yang,Wenlin LiPubMed 36477591
- Science signaling 15:eabn90092022Yap and Taz promote osteogenesis and prevent chondrogenesis in neural crest cells in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolei Zhao,Li Tang,Tram P Le,Bao H Nguyen,Wen Chen,Mingjie Zheng,Hiroyuki Yamaguchi,Brian Dawson,Shuangjie You,Idaliz M Martinez-Traverso,Shannon Erhardt,Jianxin Wang,Min Li,James F Martin,Brendan H Lee,Yoshihiro Komatsu,Jun WangPubMed 36282910
- The Journal of biological chemistry 298:1025322022The CX3CL1 intracellular domain exhibits neuroprotection via insulin receptor/insulin-like growth factor receptor signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManoshi Gayen,Marc R Benoit,Qingyuan Fan,Jacob Hudobenko,Riqiang YanPubMed 36162508
- Gut and liver 17:389-4032022circ-LDLRAD3 Knockdown Reduces Cisplatin Chemoresistance and Inhibits the Development of Gastric Cancer with Cisplatin Resistance through miR-588 Enrichment-Mediated SOX5 Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianping Liang,Feifei Chu,Lei Zhang,Yuanyuan Jiang,Lu Li,Huili WuPubMed 35975639
- Journal of leukocyte biology 112:1387-13972022Selenoprotein I deficiency in T cells promotes differentiation into tolerant phenotypes while decreasing Th17 pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Ma,FuKun W Hoffmann,Lance G Nunes,Frank Urena,Anastasia Andrukhiv,Mariana Gerschenson,Matthew W Pitts,Peter R HoffmannPubMed 35916034
- Translational psychiatry 12:2892022Cell-type specific changes in PKC-delta neurons of the central amygdala during alcohol withdrawal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeoffrey A Dilly,Cory W Kittleman,Tony M Kerr,Robert O Messing,R Dayne MayfieldPubMed 35859068
- eLife 11:2022Pum2 and TDP-43 refine area-specific cytoarchitecture post-mitotically and modulate translation of , and mRNAs in developing mouse neocortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKawssar Harb,Melanie Richter,Nagammal Neelagandan,Elia Magrinelli,Hend Harfoush,Katrin Kuechler,Melad Henis,Irm Hermanns-Borgmeyer,Froylan Calderon de Anda,Kent DuncanPubMed 35262486
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Expected | Expected | Expected | Tested |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Pig | Dilution info - | Notes - |
Target data
Function
Transcription factor involved in chondrocytes differentiation and cartilage formation. Specifically binds the 5'-AACAAT-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes, such as COL2A1 and AGC1. Required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes: SOX5 and SOX6 cooperatively bind with SOX9 on active enhancers and super-enhancers associated with cartilage-specific genes, and thereby potentiate SOX9's ability to transactivate. Not involved in precartilaginous condensation, the first step in chondrogenesis, during which skeletal progenitors differentiate into prechondrocytes. Together with SOX6, required to form and maintain a pool of highly proliferating chondroblasts between epiphyses and metaphyses, to form columnar chondroblasts, delay chondrocyte prehypertrophy but promote hypertrophy, and to delay terminal differentiation of chondrocytes on contact with ossification fronts. Binds to the proximal promoter region of the myelin protein MPZ gene.
Alternative names
Transcription factor SOX-5, SOX5
Recommended products
Anti-SOX5 antibody (ab94396) is a rabbit polyclonal antibody that is used to detect SOX5 in Western Blot, IP, IHC-P, IHC-Fr, ChIP. Suitable for Human, Mouse samples.
- Over 30 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of ab94396 are tested in WB. Previous batches were additionally validated in ChIP and IHC-Fr. These applications are still expected to work and are covered by our Abpromise guarantee.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-SOX5 antibody (ab94396) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ChIP in Human, Mouse samples.
What is the molecular weight of SOX5?
Anti-SOX5 (ab94396) specifically detects a band for SOX5 (UniProt: P35711) at a molecular weight of 84kDa.
Trusted by the scientific community
Anti-SOX5 (ab94396) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-SOX5 (ab94396) has over 5 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry paraffin embedded sections - Anti-SOX5 antibody (ab94396)
Immunohistochemistry image of SOX5 staining in a section of formalin-fixed paraffin-embedded normal e17 mouse embryonic brain performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab94396, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.The inset secondary-only control image is taken from an identical assay without primary antibody.
For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-SOX5 antibody (ab94396)
All lanes: Western blot - Anti-SOX5 antibody (ab94396) at 1 µg/mL
All lanes: Human brain tissue lysate - total protein (ab29466) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 24 kDa, 28 kDa, 55 kDa, 84 kDa
Exposure time: 5min
Immunohistochemistry paraffin embedded sections - Anti-SOX5 antibody (ab94396)
Immunohistochemistry image of SOX5 staining in a section of formalin-fixed paraffin-embedded normal e17 mouse cartilage performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab94396, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen.The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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