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AB94396

Anti-SOX5 antibody

5

(5 Reviews)

|

(43 Publications)

Anti-SOX5 antibody (ab94396) is a rabbit polyclonal antibody detecting SOX5 in Western Blot, IP, IHC-P, IHC-Fr, ChIP. Suitable for Human, Mouse.

- Over 30 publications

View Alternative Names

Transcription factor SOX-5, SOX5

3 Images
Immunohistochemistry paraffin embedded sections - Anti-SOX5 antibody (AB94396)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SOX5 antibody (AB94396)

Immunohistochemistry image of SOX5 staining in a section of formalin-fixed paraffin-embedded normal e17 mouse embryonic brain performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab94396, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry paraffin embedded sections - Anti-SOX5 antibody (AB94396)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SOX5 antibody (AB94396)

Immunohistochemistry image of SOX5 staining in a section of formalin-fixed paraffin-embedded normal e17 mouse cartilage performed on a Leica Biosystems BOND® RX instrument using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab94396, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen.The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other Immunohistochemistry staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-SOX5 antibody (AB94396)
  • WB

Unknown

Western blot - Anti-SOX5 antibody (AB94396)

All lanes:

Western blot - Anti-SOX5 antibody (ab94396) at 1 µg/mL

All lanes:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 84 kDa

Observed band size: 24 kDa,28 kDa,55 kDa,84 kDa

true

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

IHC-Fr, WB, ChIP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of ab94396 are tested in WB. Previous batches were additionally validated in ChIP and IHC-Fr. These applications are still expected to work and are covered by our Abpromise guarantee.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-SOX5 antibody (ab94396) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ChIP in Human, Mouse samples.

What is the molecular weight of SOX5?
Anti-SOX5 (ab94396) specifically detects a band for SOX5 (UniProt: P35711) at a molecular weight of 84kDa.

Trusted by the scientific community
Anti-SOX5 (ab94396) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-SOX5 (ab94396) has over 5 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in chondrocytes differentiation and cartilage formation. Specifically binds the 5'-AACAAT-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes, such as COL2A1 and AGC1. Required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes : SOX5 and SOX6 cooperatively bind with SOX9 on active enhancers and super-enhancers associated with cartilage-specific genes, and thereby potentiate SOX9's ability to transactivate. Not involved in precartilaginous condensation, the first step in chondrogenesis, during which skeletal progenitors differentiate into prechondrocytes. Together with SOX6, required to form and maintain a pool of highly proliferating chondroblasts between epiphyses and metaphyses, to form columnar chondroblasts, delay chondrocyte prehypertrophy but promote hypertrophy, and to delay terminal differentiation of chondrocytes on contact with ossification fronts. Binds to the proximal promoter region of the myelin protein MPZ gene.
See full target information SOX5
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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8480 PubMed41006212

2025

Circular RNA-based protein replacement therapy mitigates osteoarthritis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jinlong Suo,Ling Li,Wuyuan Tan,Xubin Yin,Jinghui Wang,Rui Shao,Shaokun Sun,Si-Kun Guo,Jingyi Feng,Bao-Qing Gao,Ying Wang,Meng-Yuan Wei,Lijun Wang,Heng Feng,Xiang Gao,Ping Hu,Xianyou Zheng,Ling-Ling Chen,Guanghua Lei,Youkui Huang,Weiguo Zou

Frontiers in pharmacology 16:1605531 PubMed40697663

2025

Dihydroartemisinin targets the miR-497-5p/SOX5 axis to suppress tumor progression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Hua Yin,Qiang Zhou,Jian-Bing Hu,Jie Weng,Er-Dong Shen,Fang Wen,Song-Lian Liu,Lei-Lan Yin,Ya-Jun Tong,Ling Long,Ke-Wei Tang,Si-Te Bai,Lu-Di Ou

Frontiers in neuroscience 19:1546397 PubMed40370659

2025

Laminar organization of the anterior olfactory nucleus-the interplay between neurogenesis timing and neuroblast migration.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Martin-Lopez,Bowen Brennan,Marion Lefèvre,Natalie J Spence,Kimberly Han,Charles A Greer

Histology and histopathology 40:891-899 PubMed39463328

2024

Cancer-associated fibroblast-secreted exosomes promote prostate cancer cell migration and invasion by the FGL1/SOX5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingquan Kong,Xing Wang,Yu Li,Xiansheng Zhang

Communications biology 7:10 PubMed38172644

2024

STAT3 and SOX-5 induce BRG1-mediated chromatin remodeling of RORCE2 in Th17 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Wang,Chao Han,Di Yang,Jian Zhou,Hui Dong,Zhiyuan Wei,Shuai Xu,Chen Xu,Yiwei Zhang,Yi Sun,Bing Ni,Sheng Guo,Jingbo Zhang,Tingting Zhao,Xiangmei Chen,Jie Luo,Yuzhang Wu,Yi Tian

Proceedings of the National Academy of Sciences of the United States of America 120:e2220918120 PubMed37406098

2023

Origin and development of the claustrum in rhesus macaque.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Li,Alvaro Duque,Pasko Rakic

Cells 12: PubMed36672166

2023

Early Regional Patterning in the Human Prefrontal Cortex Revealed by Laminar Dynamics of Deep Projection Neuron Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Janja Kopić,Alisa Junaković,Iva Salamon,Mladen-Roko Rasin,Ivica Kostović,Željka Krsnik

Brain structure & function 228:613-633 PubMed36592215

2023

Laminar dynamics of deep projection neurons and mode of subplate formation are hallmarks of histogenetic subdivisions of the human cingulate cortex before onset of arealization.

Applications

Unspecified application

Species

Unspecified reactive species

Alisa Junaković,Janja Kopić,Alvaro Duque,Pasko Rakic,Željka Krsnik,Ivica Kostović

NPJ Regenerative medicine 7:69 PubMed36477591

2022

Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Shen,Lu Chen,Dahe Zhang,Simo Xia,Zhuman Lv,Duohong Zou,Zhiyuan Zhang,Chi Yang,Wenlin Li

Science signaling 15:eabn9009 PubMed36282910

2022

Yap and Taz promote osteogenesis and prevent chondrogenesis in neural crest cells in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Zhao,Li Tang,Tram P Le,Bao H Nguyen,Wen Chen,Mingjie Zheng,Hiroyuki Yamaguchi,Brian Dawson,Shuangjie You,Idaliz M Martinez-Traverso,Shannon Erhardt,Jianxin Wang,Min Li,James F Martin,Brendan H Lee,Yoshihiro Komatsu,Jun Wang
View all publications
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Product promise

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