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AB250630

Anti-SOX9 antibody [EPR12755] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SOX9 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Transcription factor SOX-9, SOX9

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)

This data was developed using ab182579, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Caco 2 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.

Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)

This data was developed using ab182579, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of SW480 cells labeling SOX9 using ab182579 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde.

Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)

This data was developed using ab182579, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of SW480 (human colorectal adenocarcinoma) cells labeling SOX9 with purified ab182579 at 1/500 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunoprecipitation - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)
  • IP

Lab

Immunoprecipitation - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)

This data was developed using ab182579, the same antibody clone in a different buffer formulation.

All lanes:

Immunoprecipitation - Anti-SOX9 antibody [EPR12755] (<a href='/en-us/products/primary-antibodies/sox9-antibody-epr12755-ab182579'>ab182579</a>) at 1/80 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate, 10ug

Lane 2:

SW480, 350ug, +, <a href='/en-us/products/primary-antibodies/sox9-antibody-epr12755-ab182579'>ab182579</a> 2ug

Lane 3:

SW480 cell lysate, 350ug + rabbit IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) , 2ug

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 75 kDa

false

Immunoprecipitation - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)
  • IP

Supplier Data

Immunoprecipitation - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)

This data was developed using ab182579, the same antibody clone in a different buffer formulation.

Detection of SOX9 by Western Blot of Immunprecipitate.
Caco 2 cell lysate immunoprecipitated using ab182579 at 1/50 dilution.

A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-SOX9 antibody [EPR12755] (<a href='/en-us/products/primary-antibodies/sox9-antibody-epr12755-ab182579'>ab182579</a>)

Predicted band size: 56 kDa

false

Western blot - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)
  • WB

Supplier Data

Western blot - Anti-SOX9 antibody [EPR12755] - BSA and Azide free (AB250630)

This data was developed using ab182579, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SOX9 antibody [EPR12755] (<a href='/en-us/products/primary-antibodies/sox9-antibody-epr12755-ab182579'>ab182579</a>) at 1/10000 dilution

Lane 1:

SW480 cell lysate at 20 µg

Lane 2:

Caco 2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12755

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB253226

Human SOX9 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SOX9 ELISA Kit (AB253226)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB34712

Anti-Collagen II antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

  • 4
  • 16
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250630 is the carrier-free version of ab182579.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed : 24038782). Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed : 8640233). Also binds to some promoter regions (By similarity). Plays a central role in successive steps of chondrocyte differentiation (By similarity). Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity). Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity). Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes : maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity). Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity). Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells : when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity). Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity). In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells : involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity). Controls epithelial branching during kidney development (By similarity).
See full target information Transcription factor SOX-9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com