AB238053

Anti-SOX9 antibody [PCRP-SOX9-1A2]

Name: Anti-SOX9 antibody [PCRP-SOX9-1A2] (ab238053) | Abcam
Brand: Abcam Limited
SKU: AB238053
Price: 500 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Mouse Monoclonal SOX9 antibody. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Transcription factor SOX-9.

View Alternative Names

Transcription factor SOX-9, SOX9

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [PCRP-SOX9-1A2] (AB238053)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [PCRP-SOX9-1A2] (AB238053)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for SOX9 using ab238053 at 4 μg/mL in immunohistochemical analysis.

Protein Array - Anti-SOX9 antibody [PCRP-SOX9-1A2] (AB238053)

Protein Array

Supplier Data

Protein Array - Anti-SOX9 antibody [PCRP-SOX9-1A2] (AB238053)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab238053.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PCRP-SOX9-1A2

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Transcription factor SOX-9.

P48436

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p>Incubate with primary ab for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Titi": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "predicted", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Ab purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed : 24038782). Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed : 8640233). Also binds to some promoter regions (By similarity). Plays a central role in successive steps of chondrocyte differentiation (By similarity). Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity). Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity). Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes : maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity). Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity). Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells : when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity). Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity). In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells : involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity). Controls epithelial branching during kidney development (By similarity).

See full target information Transcription factor SOX-9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:749 PubMed39820504

2025

A CD26 tendon stem progenitor cell population contributes to tendon repair and heterotopic ossification.

Applications

Unspecified application

Species

Unspecified reactive species

Siwen Chen,Yingxin Lin,Hao Yang,Zihao Li,Sifang Li,Dongying Chen,Wenjun Hao,Shuai Zhang,Hua Chao,Jingyu Zhang,Jianru Wang,Zemin Li,Xiang Li,Zhongping Zhan,Hui Liu

Neuron 110:1656-1670.e12 PubMed35276083

2022

Excessive release of inorganic polyphosphate by ALS/FTD astrocytes causes non-cell-autonomous toxicity to motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Arredondo,Carolina Cefaliello,Agnieszka Dyrda,Nur Jury,Pablo Martinez,Iván Díaz,Armando Amaro,Helene Tran,Danna Morales,Maria Pertusa,Lorelei Stoica,Elsa Fritz,Daniela Corvalán,Sebastián Abarzúa,Maxs Méndez-Ruette,Paola Fernández,Fabiola Rojas,Meenakshi Sundaram Kumar,Rodrigo Aguilar,Sandra Almeida,Alexandra Weiss,Fernando J Bustos,Fernando González-Nilo,Carolina Otero,Maria Florencia Tevy,Daryl A Bosco,Juan C Sáez,Thilo Kähne,Fen-Biao Gao,James D Berry,Katharine Nicholson,Miguel Sena-Esteves,Rodolfo Madrid,Diego Varela,Martin Montecino,Robert H Brown,Brigitte van Zundert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SOX9 antibody [PCRP-SOX9-1A2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [PCRP-SOX9-1A2] (AB238053)

Protein Array - Anti-SOX9 antibody [PCRP-SOX9-1A2] (AB238053)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise