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AB157123

Anti-SP1 antibody

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(7 Publications)

Goat Polyclonal SP1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SP1 aa 700 to C-terminus.

View Alternative Names

TSFP1, SP1, Transcription factor Sp1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB157123)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB157123)

Paraffin embedded human ovarian carcinoma tissue stained for SP1 using ab157123 at 1/5000 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-SP1 antibody (AB157123)
  • IP

Unknown

Immunoprecipitation - Anti-SP1 antibody (AB157123)

Detection of SP1 by Western Blot of Immunprecipitate.
ab157123 at 1 μg/ml labeling SP1 in 293T whole cell lysate immunoprecipitated using ab157123 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SP1 antibody (ab157123)

Predicted band size: 81 kDa

false

Western blot - Anti-SP1 antibody (AB157123)
  • WB

Supplier Data

Western blot - Anti-SP1 antibody (AB157123)

All lanes:

Western blot - Anti-SP1 antibody (ab157123) at 0.4 µg/mL

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

Renca whole cell lysate at 50 µg

Lane 3:

TCMK1 whole cell lysate at 50 µg

Predicted band size: 81 kDa

true

Exposure time: 3min

Western blot - Anti-SP1 antibody (AB157123)
  • WB

Unknown

Western blot - Anti-SP1 antibody (AB157123)

All lanes:

Western blot - Anti-SP1 antibody (ab157123) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 81 kDa

true

Exposure time: 3min

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human SP1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebra finch": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab157123 is affinity purified using an epitope specific to SP1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed : 10391891, PubMed : 11371615, PubMed : 11904305, PubMed : 14593115, PubMed : 16377629, PubMed : 16478997, PubMed : 16943418, PubMed : 17049555, PubMed : 18171990, PubMed : 18199680, PubMed : 18239466, PubMed : 18513490, PubMed : 18619531, PubMed : 19193796, PubMed : 20091743, PubMed : 21046154, PubMed : 21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
See full target information SP1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:1464-1478 PubMed36601865

2023

Downregulated mitochondrial transcription factor A enhances mycoplasma infection to promote the metastasis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yinping Wang,Gang Wang,Xin Hong,Jing Zhao,Dan Wu,Lin Chen,Xiaoli Liu,Deyu Kong,Qichao Huang,Jinliang Xing,Nan Wang,Yilin Zhao

Redox biology 47:102142 PubMed34598017

2021

Procaspase-1 patrolled to the nucleus of proatherogenic lipid LPC-activated human aortic endothelial cells induces ROS promoter CYP1B1 and strong inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Lu,Gayani Nanayakkara,Yu Sun,Lu Liu,Keman Xu,Charles Drummer,Ying Shao,Fatma Saaoud,Eric T Choi,Xiaohua Jiang,Hong Wang,Xiaofeng Yang

Frontiers in cell and developmental biology 9:647713 PubMed33791306

2021

Low-Dose Decitabine Augments the Activation and Anti-Tumor Immune Response of IFN-γ CD4 T Cells Through Enhancing IκBα Degradation and NF-κB Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Liang Dong,Jiejie Liu,Chunmeng Wang,Yan Zhang,Qian Mei,Weidong Han,Ping Xie,Jing Nie

PLoS pathogens 16:e1008752 PubMed32760121

2020

Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Bosso,Caterina Prelli Bozzo,Dominik Hotter,Meta Volcic,Christina M Stürzel,Annika Rammelt,Yi Ni,Stephan Urban,Miriam Becker,Mario Schelhaas,Sabine Wittmann,Maria H Christensen,Florian I Schmidt,Thomas Gramberg,Konstantin M J Sparrer,Daniel Sauter,Frank Kirchhoff

Journal of cellular and molecular medicine 24:10876-10888 PubMed32743904

2020

The inhibition of microRNA-326 by SP1/HDAC1 contributes to proliferation and metastasis of osteosarcoma through promoting SMO expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Hu Huang,Yang Xu,Fei-Yue Lin

Bioscience reports 39: PubMed31270251

2019

Association of rs1353058818 and rs1053004 gene polymorphisms with human tongue squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Heqing Lai,Guochao Xu,Haifeng Meng,Haiying Zhu

Cell host & microbe 25:858-872.e13 PubMed31175045

2019

IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Dominik Hotter,Matteo Bosso,Kasper L Jønsson,Christian Krapp,Christina M Stürzel,Atze Das,Elisabeth Littwitz-Salomon,Ben Berkhout,Alina Russ,Sabine Wittmann,Thomas Gramberg,Yue Zheng,Laura J Martins,Vicente Planelles,Martin R Jakobsen,Beatrice H Hahn,Ulf Dittmer,Daniel Sauter,Frank Kirchhoff
View all publications
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