Rabbit Polyclonal SP1 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 38 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.
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- Cell death & disease 14:8162023Inhibition of PFKP in renal tubular epithelial cell restrains TGF-β induced glycolysis and renal fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Yang,Han Wu,Yanchun Li,Lixin Li,Jiaqing Xiang,Lin Kang,Guangyan Yang,Zhen LiangPubMed 38086793
- Cell reports 42:1132882023Naringenin improves muscle endurance via activation of the Sp1-ERRγ transcriptional axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenyu Lv,Jiao Meng,Sheng Yao,Fu Xiao,Shilong Li,Haoyang Shi,Chen Cui,Kaixian Chen,Xiaomin Luo,Yang Ye,Chang ChenPubMed 37874675
- Journal of cellular and molecular medicine 27:1664-16812023Lyn-mediated glycolysis enhancement of microglia contributes to neuropathic pain through facilitating IRF5 nuclear translocation in spinal dorsal horn.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErliang Kong,Yongchang Li,Peng Ma,Yixuan Zhang,Ruifeng Ding,Tong Hua,Mei Yang,Hongbin YuanPubMed 37132040
- Cell death & disease 14:1462023Melatonin-mediated FKBP4 downregulation protects against stress-induced neuronal mitochondria dysfunctions by blocking nuclear translocation of GR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jeong Kim,Gee Euhn Choi,Chang Woo Chae,Jae Ryong Lim,Young Hyun Jung,Jee Hyeon Yoon,Ji Yong Park,Ho Jae HanPubMed 36810730
- Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 25:706-7202022miRNA-576-5p promotes endometrial cancer cell growth and metastasis by targeting ZBTB4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Chen,Qing Zhang,Beihua KongPubMed 36538280
- Physiological reports 10:e154942022Identification of circulating microvesicle-encapsulated miR-223 as a potential novel biomarker for ARDS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSultan Almuntashiri,Yohan Han,Hannah A Youngblood,Aaron Chase,Yin Zhu,Xiaoyun Wang,Daniel F Linder,Budder Siddiqui,Andrea Sikora,Yutao Liu,Duo ZhangPubMed 36353917
- Antioxidants (Basel, Switzerland) 11:2022Induction of Heme Oxygenase-1 by 15d-Prostaglandin J Mediated via a ROS-Dependent Sp1 and AP-1 Cascade Suppresses Lipopolysaccharide-Triggered Interleukin-6 Expression in Mouse Brain Microvascular Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChien-Chung Yang,Li-Der Hsiao,Ya-Fang Shih,Ching-I Chang,Chuen-Mao YangPubMed 35453404
- Theranostics 12:842-8582022FcγRIIB potentiates differentiation of myeloid-derived suppressor cells to mediate tumor immunoescape.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Wu,Yanquan Xu,Huakan Zhao,Yu Zhou,Yu Chen,Shuai Yang,Juan Lei,Jiangang Zhang,Jingchun Wang,Yongzhong Wu,Yongsheng LiPubMed 34976216
- eLife 10:2021Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaori Fukao,Kei Haniuda,Hiromasa Tamaki,Daisuke KitamuraPubMed 34693907
- Experimental and therapeutic medicine 22:14592021Specificity protein 1-activated bone marrow stromal cell antigen 2 accelerates pancreatic cancer cell proliferation and migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Lei,Yafeng Hou,Jiong ChenPubMed 34737799
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information. Database link P08047
Consider this alternative
Reactivity data
Select an application| IP | ChIP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/20000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed:10391891, PubMed:11371615, PubMed:11904305, PubMed:14593115, PubMed:16377629, PubMed:16478997, PubMed:16943418, PubMed:17049555, PubMed:18171990, PubMed:18199680, PubMed:18239466, PubMed:18513490, PubMed:18619531, PubMed:19193796, PubMed:20091743, PubMed:21046154, PubMed:21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
Alternative names
TSFP1, SP1, Transcription factor Sp1
Recommended products
Rabbit Polyclonal SP1 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 38 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information. Database link P08047
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
ChIP - Anti-SP1 antibody (ab227383)
ChIP was performed with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
Western blot - Anti-SP1 antibody (ab227383)
All lanes: Western blot - Anti-SP1 antibody (ab227383) at 1/10000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2: SP1 shRNA transfected HEK-293T whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody (ab227383)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SP1 (green) using ab227383 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Red: phalloidin, a cytoskeleton marker, diluted at 1/200.
Immunoprecipitation - Anti-SP1 antibody (ab227383)
SP1 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell lysate with ab227383. Western blot was performed from the immunoprecipitate using ab227383. Anti-rabbit IgG was used as secondary antibody.
Lane 1: THP-1 whole cell lysate (Input).
Lane 2: Rabbit IgG instead of ab227383 in THP-1 whole cell lysate.
Lane 3: ab227383 IP in THP-1 whole cell lysate.
All lanes: Immunoprecipitation - Anti-SP1 antibody (ab227383)
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (ab227383)
Paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (ab227383)
Paraffin-embedded C2C12 (mouse myoblast cell line) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-SP1 antibody (ab227383)
7.5% SDS-PAGE
All lanes: Western blot - Anti-SP1 antibody (ab227383) at 1/2000 dilution
All lanes: THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
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