Anti-SP1 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody (AB227383)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SP1 protein at nucleus using ab227383 at a 1/100 dilution (Green). anti alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB227383)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for SP1 protein at nucleus with ab227383 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB227383)

Paraffin-embedded HeLa (human epithelial cell line from cervix adenocarcinoma) xenograft stained for SP1 using ab227383 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB227383)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue stained for SP1 protein at cytoplasm and nucleus with ab227383 at a 1/2000 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody (AB227383)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SP1 protein at nucleus using ab227383 at a 1/1000 dilution (Green). Phalloidin, a cytoskeleton marker stained by anti-phalloidin antibody at a 1/200 dilution (Red).

• IP

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Immunoprecipitation - Anti-SP1 antibody (AB227383)

SP1 was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell lysate with ab227383. Western blot was performed from the immunoprecipitate using ab227383. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : THP-1 whole cell lysate (Input).

Lane 2 : Rabbit IgG instead of ab227383 in THP-1 whole cell lysate.

Lane 3 : ab227383 IP in THP-1 whole cell lysate.

All lanes:

Immunoprecipitation - Anti-SP1 antibody (ab227383)

Predicted band size: 81 kDa

false

• ChIP

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ChIP - Anti-SP1 antibody (AB227383)

ChIP was performed with HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen)chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.

• WB

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Western blot - Anti-SP1 antibody (AB227383)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-SP1 antibody (ab227383) at 1/2000 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 110 kDa

false

• WB

Supplier Data

Western blot - Anti-SP1 antibody (AB227383)

All lanes:

Western blot - Anti-SP1 antibody (ab227383) at 1/10000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

SP1 shRNA transfected HEK-293T whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 81 kDa

true