AB308364

Anti-SP1 antibody [HL1396]

Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal SP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.

View Alternative Names

TSFP1, SP1, Transcription factor Sp1

8 Images

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SP1 protein using ab308364 at 1/500 dilution (green). Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of rat intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-SP1 antibody [HL1396] (AB308364)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (ab308364) diluted at 1 : 10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-SP1 antibody [HL1396] (ab308364) at 1/10000 dilution

Lane 1:

K562 cell lysate at 30 µg

Lane 2:

THP1 cell lysate at 30 µg

Lane 3:

HL60 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 80.7 kDa

false

Supplier Data

Western blot - Anti-SP1 antibody [HL1396] (AB308364)

All lanes:

Western blot - Anti-SP1 antibody [HL1396] (ab308364) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract

Lane 2:

Transfected 293T whole cell extract with DDDDK-tagged SP1

Lane 3:

Transfected 293T whole cell extract with DDDDK-tagged SP2

Lane 4:

Transfected 293T whole cell extract with DDDDK-tagged SP3

Lane 5:

Transfected 293T whole cell extract with DDDDK-tagged SP4

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1396

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information.

P08047

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed : 10391891, PubMed : 11371615, PubMed : 11904305, PubMed : 14593115, PubMed : 16377629, PubMed : 16478997, PubMed : 16943418, PubMed : 17049555, PubMed : 18171990, PubMed : 18199680, PubMed : 18239466, PubMed : 18513490, PubMed : 18619531, PubMed : 19193796, PubMed : 20091743, PubMed : 21046154, PubMed : 21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).

See full target information SP1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:737 PubMed40254586

2025

Targeting GSK3β and signaling pathways in breast cancer: role of individual members of miR- 23/24/27 cluster.

Applications

Unspecified application

Species

Unspecified reactive species

Harshi Gupta,Anushka Raghubansi, Bharat,Kritika Sharma,Krittika Zutshi,Partibha Panchal,Sushant Bhattacharya,Piyush Ranjan,Gopal Puri,Neeru Saini

Folia biologica 70:219-228 PubMed39692576

2024

Effects of Different Light Environments with Varying Spectral Composition on the Axial Lengths and Scleral Specificity Protein 1 and Collagen Type I Expression in Juvenile Guinea Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbao Yuan,Yuliang Wang,Xinyu Xu,Mei Yang,Yipeng Fan,Xiaopan Shi,Lulu Sun,Mingyu Shan,Lei Ma

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SP1 antibody [HL1396]

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Western blot - Anti-SP1 antibody [HL1396] (AB308364)

Western blot - Anti-SP1 antibody [HL1396] (AB308364)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise