Rabbit Recombinant Monoclonal SP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell line samples. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.
Images
![Western blot - Anti-SP1 antibody [HL1396] (AB308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--western-blot-img429500.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SP1 antibody [HL1396] (AB308364)")
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408045.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)")
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408044.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)")
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408043.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)")
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408042.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information. Database link P08047
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Transfected cell line | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
Species Transfected cell line | Dilution info 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed:10391891, PubMed:11371615, PubMed:11904305, PubMed:14593115, PubMed:16377629, PubMed:16478997, PubMed:16943418, PubMed:17049555, PubMed:18171990, PubMed:18199680, PubMed:18239466, PubMed:18513490, PubMed:18619531, PubMed:19193796, PubMed:20091743, PubMed:21046154, PubMed:21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
Alternative names
TSFP1, SP1, Transcription factor Sp1
Recommended products
Rabbit Recombinant Monoclonal SP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell line samples. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information. Database link P08047
- Carrier free
- Yes
- Clone number
- HL1396
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--western-blot-img429500.jpg "Western blot - Anti-SP1 antibody [HL1396] (ab308364)")
Western blot - Anti-SP1 antibody [HL1396] (ab308364)
All lanes: Western blot - Anti-SP1 antibody [HL1396] (ab308364) at 1/5000 dilution
Lane 1: Non-transfected 293T whole cell extract
Lane 2: Transfected 293T whole cell extract with DDDDK-tagged SP1
Lane 3: Transfected 293T whole cell extract with DDDDK-tagged SP2
Lane 4: Transfected 293T whole cell extract with DDDDK-tagged SP3
Lane 5: Transfected 293T whole cell extract with DDDDK-tagged SP4
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 81 kDa
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408045.jpg "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)")
Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)
Immunohistochemical analysis of rat intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408044.jpg "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)")
Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)
Immunohistochemical analysis of mouse intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408043.jpg "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)")
Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)
Immunohistochemical analysis of mouse intestine tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408042.jpg "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)")
Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)
Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408040.jpg "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)")
Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)
Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
![Western blot - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--western-blot-img408046.jpg "Western blot - Anti-SP1 antibody [HL1396] (ab308364)")
Western blot - Anti-SP1 antibody [HL1396] (ab308364)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (ab308364) diluted at 1:10000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-SP1 antibody [HL1396] (ab308364) at 1/10000 dilution
Lane 1: K562 cell lysate at 30 µg
Lane 2: THP1 cell lysate at 30 µg
Lane 3: HL60 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 80.7 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunocytochemistry-immunofluorescence-img408047.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (ab308364)")
Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (ab308364)
For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SP1 protein using ab308364 at 1/500 dilution (green). Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000
![Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunohistochemistry-paraffin-embedded-sections-img408040.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (ab308364)")
![Western blot - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--western-blot-img408046.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SP1 antibody [HL1396] (ab308364)")
![Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (ab308364), expandable thumbnail](https://content.abcam.com/products/images/sp1-antibody-hl1396-ab308364--immunocytochemistry-immunofluorescence-img408047.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (ab308364)")
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