JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB59257

Anti-SP1 (phospho T453) antibody

4

(6 Reviews)

|

(37 Publications)

Rabbit Polyclonal SP1 phospho T453 antibody. Suitable for ICC, ELISA, WB, IHC-P and reacts with Human, Mouse samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human SP1 pT453 aa 400-500.

View Alternative Names

TSFP1, SP1, Transcription factor Sp1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 (phospho T453) antibody (AB59257)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 (phospho T453) antibody (AB59257)

ab59257, at 1/50 dilution, staining SP1 in paraffin embedded human brain tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

Immunocytochemistry - Anti-SP1 (phospho T453) antibody (AB59257)
  • ICC

Supplier Data

Immunocytochemistry - Anti-SP1 (phospho T453) antibody (AB59257)

Immunofluorescence analysis of HeLa cells, using ab59257 Antibody. The picture on the right is treated with the synthesized peptide.

ELISA - Anti-SP1 (phospho T453) antibody (AB59257)
  • ELISA

Supplier Data

ELISA - Anti-SP1 (phospho T453) antibody (AB59257)

ab59257 (1 : 1000) Antibody detects endogenous levels of SP1 only when phosphorylated at Thr453.

Western blot - Anti-SP1 (phospho T453) antibody (AB59257)
  • WB

Unknown

Western blot - Anti-SP1 (phospho T453) antibody (AB59257)

All lanes:

Western blot - Anti-SP1 (phospho T453) antibody (ab59257) at 1/500 dilution

Lane 1:

A549 cell extracts

Lane 2:

A549 cell extracts with immunising phospho peptide

Predicted band size: 81 kDa

Observed band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ELISA, WB, ICC, IHC-P

applications

Immunogen

Synthetic Peptide within Human SP1 pT453 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P08047

style
left-column

Alternative Products

Primary Antibodies

AB13370

Anti-SP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB13370)

  • 4
  • 13
Primary Antibodies

AB124804

Anti-SP1 antibody [EPR6662(B)]

20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody [EPR6662(B)] (AB124804)

  • 5
  • 1
Primary Antibodies

AB227383

Anti-SP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody (AB227383)

  • 0
  • 0
Primary Antibodies

AB214861

Alexa Fluor® 488 Anti-SP1 antibody [EPR6662(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SP1 antibody [EPR6662(B)] (AB214861)

  • 0
  • 0
Primary Antibodies

AB240001

Anti-SP1 antibody [EPR6662(B)] - BSA and Azide free

Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody [EPR6662(B)] - BSA and Azide free (AB240001)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB108252

Anti-ACE2 antibody [EPR4435(2)]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4435(2)] (AB108252)

  • 4
  • 10
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/5000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab59257 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed : 10391891, PubMed : 11371615, PubMed : 11904305, PubMed : 14593115, PubMed : 16377629, PubMed : 16478997, PubMed : 16943418, PubMed : 17049555, PubMed : 18171990, PubMed : 18199680, PubMed : 18239466, PubMed : 18513490, PubMed : 18619531, PubMed : 19193796, PubMed : 20091743, PubMed : 21046154, PubMed : 21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
See full target information SP1 pT453
style
left-column

Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Basic research in cardiology : PubMed40932483

2025

Cardiac sodium-glucose co-transporter 1 (SGLT1) contributes to heart failure in a mouse model of diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Li,Sydney Freiberg,Meredith L Music,Lina Gu,Sarah Nacos,Joseph P Phillips,Adil Hassan,Kamel Shibbani,Sanah S Munir,Vooha K Kumar,Luke Halligan,Mia E Michel,Benjamin F London,Ngan Bui,Michael Cicha,Valerie Buffard,E Dale Abel,Ferhaan Ahmad

The EMBO journal 44:4962-4983 PubMed40702315

2025

Deletion of EP3 prostaglandin receptor in murine macrophages aggravates diet-induced obesity by suppressing SPARC.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlong Shang,Yinxiu Li,Lu Wang,Jiao Liu,Huiwen Ren,Qian Liu,Shumin Guo,Yuhong Wang,Yubo Ma,Tianyi You,Yujun Shen,Yu Zhou,Danyang Tian,Ying Yu

Clinical and translational medicine 15:e70256 PubMed40038877

2025

Topical TYK2 inhibitor ameliorates psoriasis-like dermatitis via the AKT-SP1-NGFR-AP1 pathway in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqin Fang,Rundong Jiang,Yutong Wang,Wangqing Chen,Xiang Chen,Mingzhu Yin

Cell reports. Medicine 5:101415 PubMed38382467

2024

Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Wang,Le Zhu,Tianen Li,Xinxin Lin,Yan Zheng,Da Xu,Yu Guo,Ze Zhang,Yan Fu,Hao Wang,Xufeng Wang,Tiantian Zou,Xiaotian Shen,Lumin Zhang,Nannan Lai,Lu Lu,Lunxiu Qin,Qiongzhu Dong

eLife 13: PubMed38375778

2024

Transcriptional regulation of SARS-CoV-2 receptor ACE2 by SP1.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Han,Rong-Hua Luo,Xin-Yan Long,Li-Qiong Wang,Qian Zhu,Xin-Yue Tang,Rui Zhu,Yi-Cheng Ma,Yong-Tang Zheng,Cheng-Gang Zou

Antioxidants (Basel, Switzerland) 12: PubMed36670936

2022

Specificity Protein 1-Mediated Promotion of CXCL12 Advances Endothelial Cell Metabolism and Proliferation in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Evan R DeVallance,Christopher M Dustin,Daniel Simoes de Jesus,Imad Al Ghouleh,John C Sembrat,Eugenia Cifuentes-Pagano,Patrick J Pagano

Frontiers in oncology 11:751904 PubMed34956875

2021

Herbal NF-κB Inhibitors Sensitize Rituximab-Resistant B Lymphoma Cells to Complement-Mediated Cytolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Ge,Yiqun Du,Jianfeng Chen,Na Zhu,Jiamei Yao,Xin Zhang,Na Wang,Yujing Sun,Feng Gao,Weiguo Hu,Yingyong Hou

International journal of molecular sciences 22: PubMed34681893

2021

The Role of Nrf2 Transcription Factor and Sp1-Nrf2 Protein Complex in Glutamine Transporter SN1 Regulation in Mouse Cortical Astrocytes Exposed to Ammonia.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Dąbrowska,Katarzyna Skowrońska,Mariusz Popek,Jan Albrecht,Magdalena Zielińska

Genes, brain, and behavior 20:e12728 PubMed33641236

2021

Integrated analysis of gait parameters and gene expression profiles in a murine model of subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Yuan Zheng,Gang Lu,Zhi Qiang Xiong,Chi Kwan Leung,Xian Wei Su,Tu Li,Wai Sang Poon,Wai Yee Chan,George Kwok Chu Wong

Journal of cellular physiology 236:4420-4434 PubMed33184874

2020

ERK-mediated transcriptional activation of Dicer is involved in gemcitabine resistance of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yen-Hao Su,Tung-Wei Hsu,Hsin-An Chen,Chih-Ming Su,Ming-Te Huang,Ta-Hsien Chuang,J Leo Su,Chia-Ling Hsieh,Ching-Feng Chiu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com