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AB172626

Anti-SP17 antibody [EP6496]

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(4 Publications)

Rabbit Recombinant Monoclonal SP17 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

SP17, SPA17, Sperm surface protein Sp17, Cancer/testis antigen 22, Sp17-1, Sperm autoantigenic protein 17, CT22, Sperm protein 17

6 Images
Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] (AB172626)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] (AB172626)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SP17 with purified ab172626 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-SP17 antibody [EP6496] (AB172626)
  • WB

Lab

Western blot - Anti-SP17 antibody [EP6496] (AB172626)

SP17 is triplet protein what have been described in the literature PMID : 9986726.

All lanes:

Western blot - Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution

All lanes:

Human testis lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 22-24 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (AB172626)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (AB172626)

Immunofluorescence analysis of Jurkat cells staining SP17 with ab172626 (unpurified) at 1/100 dilution (green). DAPI nuclear staining (blue).

Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (AB172626)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (AB172626)

Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-SP17 antibody [EP6496] (AB172626)
  • WB

Supplier Data

Western blot - Anti-SP17 antibody [EP6496] (AB172626)

All lanes:

Western blot - Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Rat testis lysate at 10 µg

Lane 3:

Human testis lysate at 10 µg

Predicted band size: 17 kDa

false

Western blot - Anti-SP17 antibody [EP6496] (AB172626)
  • WB

Lab

Western blot - Anti-SP17 antibody [EP6496] (AB172626)

SP17 is triplet protein what have been described in the literature PMID : 9986726.

All lanes:

Western blot - Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution

Lane 1:

Rat testis lysates at 15 µg

Lane 2:

Mouse testis lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 22-24 kDa

false

  • Carrier free

    Anti-SP17 antibody [EP6496] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP6496

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity).
See full target information SPA17
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Asian journal of andrology 22:88-93 PubMed31006710

2019

Reduced semen quality in patients with testicular cancer seminoma is associated with alterations in the expression of sperm proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia R Dias,Ashok Agarwal,Peter N Pushparaj,Gulfam Ahmad,Rakesh Sharma

Andrology 6:345-350 PubMed29409138

2018

Treatment of semen samples with α-chymotrypsin alters the expression pattern of sperm functional proteins-a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

M K Panner Selvam,A Agarwal,R Sharma,L Samanta

Andrologia 50: PubMed29205438

2017

Towards the identification of reliable sperm biomarkers for male infertility: A sperm proteomic approach.

Applications

Unspecified application

Species

Unspecified reactive species

P Intasqui,A Agarwal,R Sharma,L Samanta,R P Bertolla

American journal of translational research 9:454-465 PubMed28337274

2017

Decitabine treatment sensitizes tumor cells to T-cell-mediated cytotoxicity in patients with myelodysplastic syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhang,Qi He,Ying Tao,Juan Guo,Feng Xu,Ling-Yun Wu,You-Shan Zhao,Dong Wu,Li-Yu Zhou,Ji-Ying Su,Lu-Xi Song,Chao Xiao,Xiao Li,Chun-Kang Chang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com