Rabbit Recombinant Monoclonal SP17 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] - BSA and Azide free (AB245780), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-ep6496-bsa-and-azide-free-ab245780--flow-cytometry-intracellular-img136342.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] - BSA and Azide free (AB245780)")
![Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (AB245780), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-ep6496-bsa-and-azide-free-ab245780--immunocytochemistry-immunofluorescence-img21419.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (AB245780)")
![Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (AB245780), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-ep6496-bsa-and-azide-free-ab245780--immunocytochemistry-immunofluorescence-img134295.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (AB245780)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Expected | Predicted | Predicted |
| Rat | Expected | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity).
Alternative names
Sperm surface protein Sp17, Cancer/testis antigen 22, Sp17-1, Sperm autoantigenic protein 17, CT22, Sperm protein 17, SP17, SPA17
Recommended products
Rabbit Recombinant Monoclonal SP17 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Alternative names
Sperm surface protein Sp17, Cancer/testis antigen 22, Sp17-1, Sperm autoantigenic protein 17, CT22, Sperm protein 17, SP17, SPA17
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP6496
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab245780 is the carrier-free version of Anti-SP17 antibody [EP6496] ab172626.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-ep6496-bsa-and-azide-free-ab245780--flow-cytometry-intracellular-img136342.jpg "Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780)")
Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780)
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SP17 with purified Anti-SP17 antibody [EP6496] ab172626 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SP17 antibody [EP6496] ab172626).
![Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-ep6496-bsa-and-azide-free-ab245780--immunocytochemistry-immunofluorescence-img21419.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780)")
Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780)
Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling SP17 with purified Anti-SP17 antibody [EP6496] ab172626 at 1/100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SP17 antibody [EP6496] ab172626).
![Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-ep6496-bsa-and-azide-free-ab245780--immunocytochemistry-immunofluorescence-img134295.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780)")
Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] - BSA and Azide free (ab245780)
Immunofluorescence analysis of Jurkat cells staining SP17 with Anti-SP17 antibody [EP6496] ab172626 (unpurified) at 1/100 dilution (green). DAPI nuclear staining (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-SP17 antibody [EP6496] ab172626).
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