AB181079

Anti-SP17 antibody [EPR6497(2)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal SP17 antibody. Suitable for IP, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

SP17, SPA17, Sperm surface protein Sp17, Cancer/testis antigen 22, Sp17-1, Sperm autoantigenic protein 17, CT22, Sperm protein 17

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (AB181079)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling SP17 with unpurified ab181079 at 1/250 dilution. Counterstained with hematoxylin.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SP17 with purified ab181079 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Supplier Data

Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Western blot analysis of SP17 in immunoprecipitation pellets from Human testis tissue lysate. Unpurified ab181079 was used at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500. Lane 2 shows negative control.

All lanes:

Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (ab181079)

Predicted band size: 17 kDa

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Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (AB181079)

ab181079 (purified) at 1/90 immunoprecipitating SP17 in human testis tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (ab181079)

Predicted band size: 17 kDa

false

Carrier free

Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6497(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/90", "IP-species-notes": "For unpurified use at 1/50.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1500", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250." }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity).

See full target information SPA17

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed39907656

2025

VPS41 recruits biosynthetic LAMP-positive vesicles through interaction with Arl8b.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Sanzà,Jan van der Beek,Derk Draper,Cecilia de Heus,Tineke Veenendaal,Corlinda Ten Brink,Ginny G Farías,Nalan Liv,Judith Klumperman

Journal of cutaneous pathology 46:748-752 PubMed31218705

2019

Cancer-testis antigens as biomarkers for Merkel cell carcinoma: Pitfalls and opportunities.

Applications

Unspecified application

Species

Unspecified reactive species

Bahar Dasgeb,Darius Mehregan,Christina Ring,Nathan Nartker,Isaac Brownell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SP17 antibody [EPR6497(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (AB181079)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise