Rabbit Recombinant Monoclonal SP17 antibody. Carrier free. Suitable for IP, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59743.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329)")
![Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunoprecipitation-img102566.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133395.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329)")
![Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunoprecipitation-img112502.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (AB250329)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Cynomolgus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
Target data
Function
Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity).
Alternative names
SP17, SPA17, Sperm surface protein Sp17, Cancer/testis antigen 22, Sp17-1, Sperm autoantigenic protein 17, CT22, Sperm protein 17
Recommended products
Rabbit Recombinant Monoclonal SP17 antibody. Carrier free. Suitable for IP, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6497(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250329 is the carrier-free version of Anti-SP17 antibody [EPR6497(2)] ab181079.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59743.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
This data was developed using Anti-SP17 antibody [EPR6497(2)] ab181079, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SP17 with purified Anti-SP17 antibody [EPR6497(2)] ab181079 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
![Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunoprecipitation-img102566.jpg "Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)")
Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
This data was developed using Anti-SP17 antibody [EPR6497(2)] ab181079, the same antibody clone in a different buffer formulation.Anti-SP17 antibody [EPR6497(2)] ab181079 (purified) at 1/90 immunoprecipitating SP17 in human testis tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (Anti-SP17 antibody [EPR6497(2)] ab181079)
Predicted band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133395.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
This data was developed using Anti-SP17 antibody [EPR6497(2)] ab181079, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling SP17 with unpurified Anti-SP17 antibody [EPR6497(2)] ab181079 at 1/250 dilution. Counterstained with hematoxylin.
![Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329), expandable thumbnail](https://content.abcam.com/products/images/sp17-antibody-epr64972-bsa-and-azide-free-ab250329--immunoprecipitation-img112502.jpg "Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)")
Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] - BSA and Azide free (ab250329)
This data was developed using Anti-SP17 antibody [EPR6497(2)] ab181079, the same antibody clone in a different buffer formulation.Western blot analysis of SP17 in immunoprecipitation pellets from Human testis tissue lysate. Unpurified Anti-SP17 antibody [EPR6497(2)] ab181079 was used at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500. Lane 2 shows negative control.
All lanes: Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (Anti-SP17 antibody [EPR6497(2)] ab181079)
Predicted band size: 17 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com