Anti-SP2 transcription factor antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP2 transcription factor antibody (AB229468)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SP2 protein at cytoplasm and nucleus using ab229468 at a 1/1000 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar= 10µm.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP2 transcription factor antibody (AB229468)

Immunohistochemical analysis of human endometrial carcinoma tissue stained for SP2 protein at nucleus with ab229468 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-SP2 transcription factor antibody (AB229468)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 65 kDa

false

• WB

Supplier Data

Western blot - Anti-SP2 transcription factor antibody (AB229468)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 65 kDa

false

• WB

Supplier Data

Western blot - Anti-SP2 transcription factor antibody (AB229468)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysate at 30 µg

Lane 2:

Jurkat nuclear extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 65 kDa

false

• WB

Supplier Data

Western blot - Anti-SP2 transcription factor antibody (AB229468)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-SP2 transcription factor antibody (ab229468) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 65 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-SP2 transcription factor antibody (AB229468)

SP2 transcription factor was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 3 μg ab229468. Western blot was performed from the immunoprecipitate using ab229468. Anti-Rabbit IgG was used as a secondary reagent.

Lane 1 : Raji whole cell extract.
Lane 2 : Control IgG IP in Raji whole cell extract.
Lane 3 : ab229468 IP in Raji whole cell extract.

All lanes:

Immunoprecipitation - Anti-SP2 transcription factor antibody (ab229468)

Predicted band size: 65 kDa

false