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AB209385

Anti-Sp5 antibody [EPR11367(2)]

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(1 Publication)

Rabbit Recombinant Monoclonal SP5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Transcription factor Sp5, SP5

1 Images
Western blot - Anti-Sp5 antibody [EPR11367(2)] (AB209385)
  • WB

Supplier Data

Western blot - Anti-Sp5 antibody [EPR11367(2)] (AB209385)

Blocking/Dilution buffer : 5% NFDM/TBST.

Overexpression lysates were kindly provided by Karl Willert, UCSD.

All lanes:

Western blot - Anti-Sp5 antibody [EPR11367(2)] (ab209385) at 1/1000 dilution

Lane 1:

Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) transfected with Sp5 construct whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 35 kDa,42 kDa

Observed band size: 40 kDa,42 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11367(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sp5 also known as SAM Pointed Domain Containing ETS Transcription Factor is a transcription factor involved in various cellular processes. It has a mass of approximately 44 kDa and is expressed in several tissues including the brain kidney and liver. Sp5 functions by binding to GC-rich motifs in DNA where it regulates gene expression. Its activity influences the transcription of target genes that are critical for developmental and cellular responses.
Biological function summary

Sp5 plays an essential role in development and cell proliferation. It does not typically form part of a larger protein complex but works on its own to modulate the expression of genes linked to the Wnt signaling pathway. In the context of embryonic development Sp5 ensures proper cell specification and tissue patterning. Dysregulation of Sp5 activity can therefore have widespread effects on organismal biology affecting multiple systems and processes.

Pathways

Sp5 participates actively in the Wnt/β-catenin signaling pathway. This pathway is critical for cellular homeostasis and embryogenesis. Sp5 works together with other proteins like TCF/LEF transcription factors to mediate Wnt target gene activation. Its regulation is critical for maintaining the balance of cellular activities impacting processes such as stem cell differentiation and self-renewal. Furthermore Sp5 has interactions with the Hedgehog signaling pathway which further connects its role in controlling cellular growth and differentiation.

Sp5 is linked to colorectal cancer and developmental disorders. These conditions arise partly due to its regulatory roles in the Wnt/β-catenin pathway. Altered expression of Sp5 can lead to inappropriate activation of genes involved in cell cycle progression and can contribute to cancer tumorigenesis. In colorectal cancer Sp5 often shows upregulated expression potentially influencing tumor progression. Its interaction with proteins like β-catenin highlights its contribution to pathway disruptions that promote disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to GC boxes promoters elements. Probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo (By similarity).
See full target information SP5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:1034 PubMed29044119

2017

The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ian J Huggins,Tomas Bos,Olivia Gaylord,Christina Jessen,Brianna Lonquich,Angeline Puranen,Jenna Richter,Charlotte Rossdam,David Brafman,Terry Gaasterland,Karl Willert
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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