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Anti-Sp7 / Osterix antibody ab22552 is a rabbit polyclonal antibody that is used in Sp7 / Osterix IHC. Suitable for mouse and rat samples.

- Tried and trusted by researchers since 2006


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Predicted
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/500

Notes

Antigen retrieval is not essential but may optimise staining.

Species

Rat

Dilution info

1/100 - 1/500

Notes

Antigen retrieval is not essential but may optimise staining.

Predicted
Predicted

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcriptional activator essential for osteoblast differentiation (PubMed:11792318, PubMed:17510056, PubMed:30026585). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (PubMed:11792318, PubMed:17510056).

Alternative names

Recommended products

Anti-Sp7 / Osterix antibody ab22552 is a rabbit polyclonal antibody that is used in Sp7 / Osterix IHC. Suitable for mouse and rat samples.

- Tried and trusted by researchers since 2006

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sp7 also known as Osterix is a transcription factor critical for bone formation. As a protein it has a mass of approximately 45 kDa. Sp7/Osterix is mainly expressed in osteoblasts cells responsible for new bone synthesis. The protein binds to specific DNA sequences regulating gene expression necessary for osteoblast differentiation and activity.

Biological function summary

Sp7 or Osterix functions as an important regulator in the development of the skeletal system. It operates as part of transcriptional regulation but does not form a complex with other proteins. By activating genes involved in bone matrix composition Sp7 drives the differentiation of precursor cells into mature osteoblasts which is an essential process for normal bone growth and maintenance.

Pathways

Sp7 works within the ossification pathway which is essential for bone development and regeneration. It closely interacts with Runx2 another transcription factor that plays a significant role in the regulation of osteoblast differentiation. The activity of Sp7 in this pathway ensures proper expression of bone-specific proteins including those encoded by the Spp1 and Calm genes which contribute to mineralization and calcium homeostasis.

Associated diseases and disorders

Mutations or dysregulation of Sp7/Osterix impact conditions like osteogenesis imperfecta and osteoporosis. In these diseases impaired or excessive bone formation can occur affecting skeletal integrity. The protein's functional relationship with Runx2 is important in these contexts as both proteins together determine the balance between bone formation and resorption which when disrupted leads to pathological skeletal changes.

Product promise

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)

    IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal rat E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)

    IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal mouse E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552), expandable thumbnail
    This image is courtesy of an anonymous collaborator

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)

    ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552), expandable thumbnail
    This image is courtesy of Jérôme AMIAUD, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives. We thank them for their important contribution to the validation of ab22552.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)

    Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)

    The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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