AB22552

Anti-Sp7 / Osterix antibody

Name: Anti-DFNA5/GSDME PE antibody [EPR19859] (ab225520) IgG | Abcam
Brand: Abcam Limited
SKU: AB22552
Price: 450 USD
Availability: InStock
Rating: 5 (24 reviews)

BOND RX™ Validated
What is this?

(24 Reviews)

(504 Publications)

Anti-Sp7 / Osterix antibody (ab22552) is a rabbit polyclonal antibody detecting Sp7 / Osterix in IHC-P. Suitable for Mouse, Rat.

- Over 420 publications
- Trusted since 2006

View Alternative Names

Osx, Sp7, Transcription factor Sp7, C22, Zinc finger protein osterix

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal rat E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.

This image is courtesy of an anonymous collaborator

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

This image is courtesy of Jérôme AMIAUD, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives. We thank them for their important contribution to the validation of ab22552.

IHC-P

AbReview6355****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

This image is courtesy of an anonymous Abreview

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal mouse E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." } } }

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Product details

Product Specifications
Anti-Sp7 / Osterix antibody (ab22552) is a rabbit polyclonal antibody and is validated for use in IHC-P in mouse, rat samples.
Anti-Sp7 / Osterix antibody (ab22552) specifically detects Sp7 / Osterix (UniProt ID: Q8TDD2; Molecular weight: 45kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Sp7 / Osterix antibody (ab22552) has high sensitivity and specificity.
Anti-Sp7 / Osterix antibody (ab22552) has been cited over 422 times in peer reviewed journals and is trusted by the scientific community.
Anti-Sp7 / Osterix antibody (ab22552) has 23 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional activator essential for osteoblast differentiation (PubMed : 11792318, PubMed : 17510056, PubMed : 30026585). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (PubMed : 11792318, PubMed : 17510056).

See full target information Sp7

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Publications (504)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:35547 PubMed41073566

2025

Total flavonoids of rhizoma drynariae improved the osteogenic and pro-angiogenic capacities of BMSCs to accelerate long bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hengjun Huang,Silu Li,Huang Zhan,Fenfang Gong,Jian Liu,Zhenya Liu,Hui Li,Chengyu Yang

International journal of oral science 17:65 PubMed40998797

2025

PLAGL1-IGF2 axis regulates osteogenesis of postnatal condyle development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrui Sun,Jingyi Xu,Yue Xu,Yili Liu,Enhui Yao,Jiahui Du,Xinquan Jiang

Communications biology 8:1309 PubMed40883591

2025

Ovalbumin-induced asthma leads to bone loss with Piezo channel suppression in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqi Gao,Takeshi Sawada,Ailin Cao,Reiko U Yoshimoto,Yu Yamaguchi,Yukie Takahashi,Takaichi Fukuda,Yasuyoshi Ohsaki,Reona Aijima,Tomoko Kadowaki,Takayuki Tsukuba,Mizuho A Kido

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Frontiers in bioengineering and biotechnology 13:1639117 PubMed40837020

2025

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Hai-Jing Zhang,Hui Wang,Gang Ji,Ya-Heng Zhao,Gao-Cen Li,Shao-Shi Guo,Lu-Feng Lin,Yu-Jie Jin,Xue-Li Zhang,Xin-Yu Nan,Chang-Cheng Liu,Guo-Bin Liu

Frontiers in endocrinology 16:1570685 PubMed40687591

2025

Jinkui Shenqi Pill accelerates osteoporotic fracture healing by promoting bone formation through neurosensory PGE2/EP4/p-CREB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihao Xi,Danqing Fu,Dan Xu,Ruhan Shen,Yan Zhao,Haoqiang Dai,Chenjie Xia,Peihong Zhou

Nature communications 16:5425 PubMed40593719

2025

Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Liu,Lu Xing,Wenlong Huang,Ning Ji,Hang Zhao,Qianming Chen,Xianglong Han,Ding Bai,Xuefeng Zhao

Organogenesis 21:2519649 PubMed40570323

2025

SS-31 Targets NOS2 to Enhance Osteogenic Differentiation in Aged BMSCs by Restoring Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Duan,Qindong Zhang,Jinqiang Zhu,Jiaming Wang

Journal of orthopaedic translation 52:387-403 PubMed40476065

2025

Vitamins D and K jointly protect against osteoarthritis via regulating OSCAR during osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Qianhua Ou,Hong Huang,Delong Li,Jianmao Chen,Song Xue,Zuoqing Zhou,Guangfeng Ruan,Changhai Ding

Nature communications 16:4330 PubMed40346043

2025

Self-propagating wave drives morphogenesis of skull bones in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yiteng Dang,Johanna Lattner,Adrian A Lahola-Chomiak,Diana Alves Afonso,Elke Ulbricht,Anna Taubenberger,Steffen Rulands,Jacqueline M Tabler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sp7 / Osterix antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (504)

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