Rabbit Recombinant Monoclonal Sp7 / Osterix antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-frozen-sections-img133067.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29909.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820)")
![Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunoprecipitation-img332.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72241.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133128.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (AB227820)")
Publications
Filter by
- iScience 26:1067792023Osteoderms in a mammal the spiny mouse and the independent evolution of dermal armor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalcolm Maden,Trey Polvadore,Arod Polanco,W Brad Barbazuk,Edward StanleyPubMed 37378333
- Cell and tissue banking 24:45-582022Low-intensity pulsed ultrasound enhances immunomodulation and facilitates osteogenesis of human periodontal ligament stem cells by inhibiting the NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan Lin,Qing Wang,Chuntian Quan,Qingyuan Ren,Wulin He,Hui XiaoPubMed 35644018
- Bone research 10:222022Spinal cord injury reprograms muscle fibroadipogenic progenitors to form heterotopic bones within muscles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsu-Wen Tseng,Dorothée Girard,Kylie A Alexander,Susan M Millard,Frédéric Torossian,Adrienne Anginot,Whitney Fleming,Jules Gueguen,Marie-Emmanuelle Goriot,Denis Clay,Beulah Jose,Bianca Nowlan,Allison R Pettit,Marjorie Salga,François Genêt,Marie-Caroline Le Bousse-Kerdilès,Sébastien Banzet,Jean-Pierre LévesquePubMed 35217633
- Regenerative therapy 18:430-4402021RUNX3 derived hsa_circ_0005752 accelerates the osteogenic differentiation of adipose-derived stem cells via the miR-496/MDM2-p53 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Wang,Yifan Huan,Xiyang Li,Jing Li,Guohua LvPubMed 34754888
- eLife 9:2020Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiancamaria Ricci et. al.PubMed 32755539
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Mouse | Expected | Not recommended | Tested | Expected |
| Rat | Expected | Not recommended | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Mouse and rat species are recommended based on the IHC result. We do not guarantee western blot for mouse and rat. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Mouse and rat species are recommended based on the IHC result. We do not guarantee western blot for mouse and rat. |
Species Rat | Dilution info - | Notes Mouse and rat species are recommended based on the IHC result. We do not guarantee western blot for mouse and rat. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional activator essential for osteoblast differentiation (PubMed:23457570). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).
Alternative names
OSX, SP7, Transcription factor Sp7, Zinc finger protein osterix
Recommended products
Rabbit Recombinant Monoclonal Sp7 / Osterix antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21034
- Purification technique
- Affinity purification Protein A
- Specificity
Mouse and rat species are recommended based on the IHC result. We do not guarantee western blot for mouse and rat.
We recommend using 1% SDS hot method to prepare lysate to improve signal intensity.
Please refer to the Protocol booklet under the Support & downloads section for the lysate preparation protocol.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227820 is the carrier-free version of Anti-Sp7 / Osterix antibody [EPR21034] ab209484.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sp7 also known as Osterix is a transcription factor critical for bone formation. As a protein it has a mass of approximately 45 kDa. Sp7/Osterix is mainly expressed in osteoblasts cells responsible for new bone synthesis. The protein binds to specific DNA sequences regulating gene expression necessary for osteoblast differentiation and activity.
- Biological function summary
Sp7 or Osterix functions as an important regulator in the development of the skeletal system. It operates as part of transcriptional regulation but does not form a complex with other proteins. By activating genes involved in bone matrix composition Sp7 drives the differentiation of precursor cells into mature osteoblasts which is an essential process for normal bone growth and maintenance.
- Pathways
Sp7 works within the ossification pathway which is essential for bone development and regeneration. It closely interacts with Runx2 another transcription factor that plays a significant role in the regulation of osteoblast differentiation. The activity of Sp7 in this pathway ensures proper expression of bone-specific proteins including those encoded by the Spp1 and Calm genes which contribute to mineralization and calcium homeostasis.
- Associated diseases and disorders
Mutations or dysregulation of Sp7/Osterix impact conditions like osteogenesis imperfecta and osteoporosis. In these diseases impaired or excessive bone formation can occur affecting skeletal integrity. The protein's functional relationship with Runx2 is important in these contexts as both proteins together determine the balance between bone formation and resorption which when disrupted leads to pathological skeletal changes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-frozen-sections-img133067.jpg "Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
Immunohistochemistry (Frozen sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse E14.5 developing humerus tissue labeling Sp7 / Osterix with Anti-Sp7 / Osterix antibody [EPR21034] ab209484 at 1/500 dilution, followed by AlexaFluor® 488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive staining on the mouse embryo E14.5 developing humerus (PMID:27134141) is observed. Counter stained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor® 488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sp7 / Osterix antibody [EPR21034] ab209484).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29909.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
Immunohistochemical analysis of paraffin-embedded rat E14.5 rib tissue labeling Sp7 / Osterix with Anti-Sp7 / Osterix antibody [EPR21034] ab209484 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on osteoblasts and chondrocytes of rat E14.5 rib (PMID: 17579353; PMID: 25977369) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sp7 / Osterix antibody [EPR21034] ab209484).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunoprecipitation-img332.jpg "Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
Sp7 / Osterix was immunoprecipitated from 0.35mg of Saos-2 (human osteosarcoma cell line) whole cell lysate with Anti-Sp7 / Osterix antibody [EPR21034] ab209484 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Sp7 / Osterix antibody [EPR21034] ab209484 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Saos-2 whole cell lysate 10ug (Input).
Lane 2: Anti-Sp7 / Osterix antibody [EPR21034] ab209484 IP in Saos-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] ab190908 in Saos-2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 8 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sp7 / Osterix antibody [EPR21034] ab209484).
All lanes: Immunoprecipitation - Anti-Sp7 / Osterix antibody [EPR21034] (Anti-Sp7 / Osterix antibody [EPR21034] ab209484)
Predicted band size: 45 kDa, 47 kDa
Observed band size: 45 kDa, 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72241.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
Immunohistochemical analysis of paraffin-embedded mouse E14.5 rib tissue labeling Sp7 / Osterix with Anti-Sp7 / Osterix antibody [EPR21034] ab209484 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on osteoblasts and chondrocytes of mouse E14.5 rib (PMID: 17579353; PMID: 25977369) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sp7 / Osterix antibody [EPR21034] ab209484).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133128.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
Immunohistochemical analysis of paraffin-embedded human chondrosarcoma tissue labeling Sp7 / Osterix with Anti-Sp7 / Osterix antibody [EPR21034] ab209484 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on tumor cells of human chondrosarcoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sp7 / Osterix antibody [EPR21034] ab209484).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--western-blot-img433002.jpg "Western blot - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
Western blot - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)
Blocking and dilution buffer concentration: 5% NFDM/TBST.We recommend using 1% SDS hot method to prepare lysate to improve signal intensity. Please refer to the Protocol booklet under the Support & downloads section.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sp7 / Osterix antibody [EPR21034] ab209484).
All lanes: Western blot - Anti-Sp7 / Osterix antibody [EPR21034] (Anti-Sp7 / Osterix antibody [EPR21034] ab209484) at 1/1000 dilution
Lane 1: Saos-2 (human osteosarcoma cell line) whole cell lysate, prepared by RIPA method at 20 µg
Lane 2: Saos-2 (human osteosarcoma cell line) whole cell lysate, prepared by 1% SDS hot method at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa, 47 kDa
Exposure time: 20s
![Western blot - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820), expandable thumbnail](https://content.abcam.com/products/images/sp7-osterix-antibody-epr21034-bsa-and-azide-free-ab227820--western-blot-img433002.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free (ab227820)")
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