Rabbit Recombinant Monoclonal SP8 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Rat, Human samples.
Images
![Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--western-blot-img441134.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916)")
![Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-frozen-sections-img401424.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916)")
![Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-img401370.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916)")
![Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-img401369.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916)")
![Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-img401368.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (AB302916)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos (By similarity).
Alternative names
Transcription factor Sp8, Specificity protein 8, SP8
Recommended products
Rabbit Recombinant Monoclonal SP8 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26224-86
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sp8 also known as Specificity protein 8 is a zinc finger transcription factor. It has a molecular weight of approximately 52 kDa. This protein is expressed primarily in developing tissues including the nervous system and limb buds. It plays an important role during embryonic development by modulating the expression of multiple target genes important for tissue differentiation.
- Biological function summary
Sp8 is involved in the regulation of several developmental processes. It functions as a transcription factor that can activate or repress gene expression influencing cell fate during embryogenesis. Sp8 interacts with other transcription factors and cofactors to form larger transcriptional complexes. This protein is also important for the development of the telencephalon and limbs playing a pivotal role in ensuring proper morphological development.
- Pathways
Sp8 is an active participant in the Wnt signaling pathway where it contributes to the regulation of gene transcription required for development. In this pathway Sp8 interacts with other factors like β-catenin to promote transcriptional activation of target genes. Sp8 also has connections to the Sonic Hedgehog (Shh) signaling pathway where it influences cell proliferation and differentiation. Both pathways are essential for embryonic development and organogenesis.
- Associated diseases and disorders
Sp8 mutations or dysregulation have been associated with intellectual disabilities and limb malformations. These conditions highlight Sp8’s role in normal neurological and skeletal development. Within these disease contexts Sp8 interacts with proteins such as GLI3 which also plays a role in the developmental abnormalities seen in some syndromes. Understanding the role of Sp8 can provide insights into the molecular mechanisms underlying these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--western-blot-img441134.jpg "Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)")
Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
This data was developed using Anti-Sp8 antibody [EPR26224-86] ab302915, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST
Low expression: spleen, lung (consistent with the predicted expression pattern).
Bands below 60 kDa may due to degradation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Sp8 antibody [EPR26224-86] (Anti-Sp8 antibody [EPR26224-86] ab302915) at 1/1000 dilution
Lane 1: Rat olfactory bulb tissue lysate at 20 µg
Lane 2: Rat spleen tissue lysate at 20 µg
Lane 3: Rat lung tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
Observed band size: 30-60 kDa
Exposure time: 180s
![Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-frozen-sections-img401424.jpg "Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)")
Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
This data was developed using Anti-Sp8 antibody [EPR26224-86] ab302915, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat olfactory bulb (fresh) tissue labeling Sp8 with Anti-Sp8 antibody [EPR26224-86] ab302915 at 1/500 (1.06 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat olfactory bulb. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sp8 antibody [EPR26224-86] ab302915 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.<\p> .
![Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-img401370.jpg "Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)")
Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
This data was developed using Anti-Sp8 antibody [EPR26224-86] ab302915, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded A HEK-293T cells tr tissue labeling Sp8 with Anti-Sp8 antibody [EPR26224-86] ab302915 at 1/5000 (0.106 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on HEK-293T cells transfected with a human SP8 expression vector containing a myc-His tag cell pallet (image A). No staining on HEK-293T cells transfected with empty vector containing a his tag cell pallet (image B). The section was incubated with Anti-Sp8 antibody [EPR26224-86] ab302915 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-img401369.jpg "Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)")
Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
This data was developed using Anti-Sp8 antibody [EPR26224-86] ab302915, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Sp8 with Anti-Sp8 antibody [EPR26224-86] ab302915 at 1/200 (2.65 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. (A) Nuclear staining on olfactory bulb of rat brain at low-powered (magnification, x10) and (B) nuclear staining on rat olfactory bulb at high-powered (magnification, x400). The section was incubated with Anti-Sp8 antibody [EPR26224-86] ab302915 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916), expandable thumbnail](https://content.abcam.com/products/images/sp8-antibody-epr26224-86-bsa-and-azide-free-ab302916--immunohistochemistry-img401368.jpg "Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)")
Immunohistochemistry - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
This data was developed using Anti-Sp8 antibody [EPR26224-86] ab302915, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Sp8 with Anti-Sp8 antibody [EPR26224-86] ab302915 at 1/50 (10.6 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human prostate. The section was incubated with Anti-Sp8 antibody [EPR26224-86] ab302915 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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