AB85928

Anti-Spa-1 antibody

Name: Anti-Spa-1 antibody (ab85928) | Abcam
Brand: Abcam Limited
SKU: AB85928
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Spa-1 antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SIPA1 aa 950 to C-terminus.

View Alternative Names

SPA1, SIPA1, Signal-induced proliferation-associated protein 1, Sipa-1, GTPase-activating protein Spa-1, p130 SPA-1

2 Images

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Immunoprecipitation - Anti-Spa-1 antibody (AB85928)

Detection of Spa-1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Spa-1 antibody (ab85928)

Predicted band size: 112 kDa

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Western blot - Anti-Spa-1 antibody (AB85928)

All lanes:

Western blot - Anti-Spa-1 antibody (ab85928) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 112 kDa

Observed band size: 117 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SIPA1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96FS4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab85928 was affinity purified using an epitope specific to Spa-1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Spa-1 also known as human SPA plays a role in the regulation of small GTPase activity specifically targeting Rap1. This protein weighing approximately 120 kDa resides within various tissues with notable expression in the hematopoietic system and neuronal tissues. Researchers use names like Anti-SPA and Anti-Spa when referring to assays designed to recognize this protein. SPA-1 functions as a Rap1 GTPase-activating protein (GAP) facilitating the conversion of active GTP-bound Rap1 to its inactive GDP-bound form.

Biological function summary

Spa-1 engages in the control of cell adhesion and migration through its interaction with the Ras-related protein Rap1. As part of larger signal transduction complexes Spa-1 influences the organization of actin cytoskeleton and impacts integrin-mediated cell adhesion. Through its GAP activity it maintains cellular responsiveness to external stimuli impacting processes like immune cell trafficking and neuronal growth. Studies have noted its significance in maintaining homeostasis in tissues where it is expressed.

Pathways

Spa-1 integrates into key signaling cascades impacting the Ras superfamily of GTPases. Within the Rap1 signaling pathway Spa-1 works alongside proteins like RapGEF to finely regulate signal transduction processes affecting cell adhesion and cytoskeletal dynamics. In the context of the integrin signaling pathway Spa-1 modulates the actions of B-Raf in connection with Rap1 dictating responses necessary for cellular motility and positioning.

Spa-1 displays relevance to leukemia and neurological conditions. Its role in negative regulation of Rap1 activity links it to the aberrant proliferation seen in certain leukemia types where altered Rap1 signaling contributes to uncontrolled cell growth. Furthermore disruptions in Spa-1's modulation of neuronal Rap1 activity have associations with neurodegenerative diseases where proper cell migration and adhesion are impaired. Research explores its connections to proteins like BCR-ABL in the context of leukemia highlighting Spa-1 as a therapeutic target in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state (PubMed : 9346962). Affects cell cycle progression (By similarity).

See full target information SIPA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107292 PubMed37520697

2023

Rap1 organizes lymphocyte front-back polarity via RhoA signaling and talin1.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Ueda,Koichiro Higasa,Yuji Kamioka,Naoyuki Kondo,Shunsuke Horitani,Yoshiki Ikeda,Wolfgang Bergmeier,Yoshinori Fukui,Tatsuo Kinashi

Journal of Cancer 14:306-317 PubMed36741255

2023

C4orf47 contributes to the dormancy of pancreatic cancer under hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Shinjiro Nagao,Hideya Onishi,Makoto Kawamoto,Shogo Masuda,Lin Na,Shinji Morisaki,Naoya Iwamoto,Yutaka Yamada,Satoko Koga,Shu Ichimiya,Kazunori Nakayama,Akira Imaizumi,Kinichi Nakashima,Yoshinao Oda,Masafumi Nakamura

Frontiers in cell and developmental biology 9:779169 PubMed35096814

2022

SIPA1 Enhances Aerobic Glycolysis Through HIF-2α Pathway to Promote Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Yao,Jun Weng,Lingyun Feng,Wanjun Zhang,Yan Xu,Peijing Zhang,Yoshimasa Tanaka,Li Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Spa-1 antibody

Immunoprecipitation - Anti-Spa-1 antibody (AB85928)

Western blot - Anti-Spa-1 antibody (AB85928)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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