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AB121768

Anti-SPACA3 antibody

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(1 Publication)

Rabbit Polyclonal SPACA3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPACA3 aa 100 to C-terminus.

View Alternative Names

LYC3, LYZL3, SLLP1, SPRASA, UNQ424/PRO862, SPACA3, Sperm acrosome membrane-associated protein 3, Cancer/testis antigen 54, Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17, Lysozyme-like protein 3, Sperm lysozyme-like protein 1, Sperm protein reactive with antisperm antibodies, CT54, Sperm protein reactive with ASA

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPACA3 antibody (AB121768)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPACA3 antibody (AB121768)

ab121768 at a 1/2500 dilution staining SPACA3 in paraffin embedded Human testis tissue by Immunohistochemistry.

Western blot - Anti-SPACA3 antibody (AB121768)
  • WB

Unknown

Western blot - Anti-SPACA3 antibody (AB121768)

Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

All lanes:

Western blot - Anti-SPACA3 antibody (ab121768)

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPACA3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IXA5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.
See full target information SPACA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of allergy and clinical immunology 130:481-8.e2 PubMed22721650

2012

LPS-responsive beige-like anchor (LRBA) gene mutation in a family with inflammatory bowel disease and combined immunodeficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Abdullah Alangari,Abdulrahman Alsultan,Nouran Adly,Michel J Massaad,Iram Shakir Kiani,Abdulrahman Aljebreen,Emad Raddaoui,Abdul-Kareem Almomen,Saleh Al-Muhsen,Raif S Geha,Fowzan S Alkuraya
View all publications
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