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Rabbit Polyclonal SPAG1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPAG1 aa 750-800.

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Images

Western blot - Anti-spag1 antibody (AB183089), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-spag1 antibody (AB183089), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human SPAG1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q07617

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/25.00000 - 1/100.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity.

Alternative names

Recommended products

Rabbit Polyclonal SPAG1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPAG1 aa 750-800.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human SPAG1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information. Database link Q07617
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The SPAG1 protein also known as Sperm Associated Antigen 1 functions mechanically as a molecular chaperone. It associates with microtubules and plays a role in their dynamics and stability. SPAG1 is a protein of around 98 kDa in mass and researchers find it in high amounts in testis tissue suggesting its involvement in spermatogenesis. However SPAG1 also expresses in other tissues although at lower levels indicating additional roles beyond reproduction.

Biological function summary

SPAG1 interacts with multiple cellular structures playing a role in microtubule-dependent processes. It forms part of a complex involving the NOI protein and other molecular chaperones. This complex works together to ensure the proper folding and function of proteins required for microtubule organization. Through these interactions SPAG1 contributes to cell division and intracellular transport providing foundational support for these cellular activities.

Pathways

Four important cellular processes involve SPAG1 including the MAPK signaling pathway. SPAG1 influences MAPK through its interaction with RAN GTPase a critical regulator of microtubule nucleation. The protein also plays a role in the JAK-STAT pathway impacting cellular growth and differentiation. These essential pathways position SPAG1 as a connecting piece among various regulatory proteins like ORC6 and BRCA1 within complex signaling networks.

Associated diseases and disorders

SPAG1 shows associations with certain cancers notably testicular cancer. Abnormal expression or mutations within the SPAG1 gene can disrupt the regulation of the MAPK pathway leading to uncontrolled cell proliferation. Moreover researchers have linked SPAG1 to ciliary dysfunctions such as primary ciliary dyskinesia due to its role in microtubule stability. In these conditions SPAG1’s functional partners like TUBG1 often exhibit altered expression further contributing to disease progression.

Product promise

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2 product images

  • Western blot - Anti-spag1 antibody (ab183089), expandable thumbnail

    Western blot - Anti-spag1 antibody (ab183089)

    All lanes: Western blot - Anti-spag1 antibody (ab183089) at 1/600 dilution

    All lanes: Human colon tissue lysate at 40 µg

    Secondary

    All lanes: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 104 kDa

    Exposure time: 2min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-spag1 antibody (ab183089), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-spag1 antibody (ab183089)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling spag1 with ab183089 at 1/25 dilution. The image on the right is treated with the immunizing peptide.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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