Rabbit Polyclonal SPAG1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPAG1 aa 750-800.
IgG
Rabbit
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity.
Sperm-associated antigen 1, HSD-3.8, Infertility-related sperm protein Spag-1, SPAG1
Rabbit Polyclonal SPAG1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPAG1 aa 750-800.
IgG
Rabbit
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The SPAG1 protein also known as Sperm Associated Antigen 1 functions mechanically as a molecular chaperone. It associates with microtubules and plays a role in their dynamics and stability. SPAG1 is a protein of around 98 kDa in mass and researchers find it in high amounts in testis tissue suggesting its involvement in spermatogenesis. However SPAG1 also expresses in other tissues although at lower levels indicating additional roles beyond reproduction.
SPAG1 interacts with multiple cellular structures playing a role in microtubule-dependent processes. It forms part of a complex involving the NOI protein and other molecular chaperones. This complex works together to ensure the proper folding and function of proteins required for microtubule organization. Through these interactions SPAG1 contributes to cell division and intracellular transport providing foundational support for these cellular activities.
Four important cellular processes involve SPAG1 including the MAPK signaling pathway. SPAG1 influences MAPK through its interaction with RAN GTPase a critical regulator of microtubule nucleation. The protein also plays a role in the JAK-STAT pathway impacting cellular growth and differentiation. These essential pathways position SPAG1 as a connecting piece among various regulatory proteins like ORC6 and BRCA1 within complex signaling networks.
SPAG1 shows associations with certain cancers notably testicular cancer. Abnormal expression or mutations within the SPAG1 gene can disrupt the regulation of the MAPK pathway leading to uncontrolled cell proliferation. Moreover researchers have linked SPAG1 to ciliary dysfunctions such as primary ciliary dyskinesia due to its role in microtubule stability. In these conditions SPAG1’s functional partners like TUBG1 often exhibit altered expression further contributing to disease progression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-spag1 antibody (ab183089) at 1/600 dilution
All lanes: Human colon tissue lysate at 40 µg
All lanes: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 104 kDa
Exposure time: 2min
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling spag1 with ab183089 at 1/25 dilution. The image on the right is treated with the immunizing peptide.
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