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AB183089

Anti-spag1 antibody

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(1 Publication)

Rabbit Polyclonal SPAG1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPAG1 aa 750-800.

View Alternative Names

Sperm-associated antigen 1, HSD-3.8, Infertility-related sperm protein Spag-1, SPAG1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-spag1 antibody (AB183089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-spag1 antibody (AB183089)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling spag1 with ab183089 at 1/25 dilution. The image on the right is treated with the immunizing peptide.

Western blot - Anti-spag1 antibody (AB183089)
  • WB

Supplier Data

Western blot - Anti-spag1 antibody (AB183089)

All lanes:

Western blot - Anti-spag1 antibody (ab183089) at 1/600 dilution

All lanes:

Human colon tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 104 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SPAG1 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

Q07617

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB182610

Anti-Thymidine Kinase 1/TK1 (phospho S13) antibody

Western blot - Anti-Thymidine Kinase 1/TK1 (phospho S13) antibody (AB182610)

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Primary Antibodies

AB182626

Anti-ATRIP (phospho S68) antibody

Western blot - Anti-ATRIP (phospho S68) antibody (AB182626)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SPAG1 protein also known as Sperm Associated Antigen 1 functions mechanically as a molecular chaperone. It associates with microtubules and plays a role in their dynamics and stability. SPAG1 is a protein of around 98 kDa in mass and researchers find it in high amounts in testis tissue suggesting its involvement in spermatogenesis. However SPAG1 also expresses in other tissues although at lower levels indicating additional roles beyond reproduction.
Biological function summary

SPAG1 interacts with multiple cellular structures playing a role in microtubule-dependent processes. It forms part of a complex involving the NOI protein and other molecular chaperones. This complex works together to ensure the proper folding and function of proteins required for microtubule organization. Through these interactions SPAG1 contributes to cell division and intracellular transport providing foundational support for these cellular activities.

Pathways

Four important cellular processes involve SPAG1 including the MAPK signaling pathway. SPAG1 influences MAPK through its interaction with RAN GTPase a critical regulator of microtubule nucleation. The protein also plays a role in the JAK-STAT pathway impacting cellular growth and differentiation. These essential pathways position SPAG1 as a connecting piece among various regulatory proteins like ORC6 and BRCA1 within complex signaling networks.

SPAG1 shows associations with certain cancers notably testicular cancer. Abnormal expression or mutations within the SPAG1 gene can disrupt the regulation of the MAPK pathway leading to uncontrolled cell proliferation. Moreover researchers have linked SPAG1 to ciliary dysfunctions such as primary ciliary dyskinesia due to its role in microtubule stability. In these conditions SPAG1’s functional partners like TUBG1 often exhibit altered expression further contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity.
See full target information SPAG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Chemical Society 146:13676-13688 PubMed38693710

2024

Affinity-Driven Aryl Diazonium Labeling of Peptide Receptors on Living Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sheryl Sharma,Michael J Naldrett,Makayla J Gill,James W Checco
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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