JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122008

Anti-SPAG17 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal SPAG17 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPAG17 aa 1400-1550.

View Alternative Names

Sperm-associated antigen 17, Projection protein PF6 homolog, SPAG17

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SPAG17 with ab122008 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling SPAG17 with ab122008 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SPAG17 with ab122008 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG17 antibody (AB122008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling SPAG17 with ab122008 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SPAG17 aa 1400-1550. The exact immunogen used to generate this antibody is proprietary information.

Q6Q759

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPAG17 also known as sperm-associated antigen 17 is a protein with an approximate mass of 220 kDa. This protein is mainly expressed in testicular tissues and is abundant in sperm cells as well as other ciliated cells in the respiratory tract. Mechanically SPAG17 is a structural component of the axoneme a core part of cilia and flagella where it plays a role in maintaining the integrity of these structures.
Biological function summary

There exist strong associations between the workings of SPAG17 and the motility of sperm and cilia. It is a part of the central apparatus complex of the axoneme which is important for normal ciliary and flagellar beat patterns. In sperm cells SPAG17 impacts sperm motility greatly influencing male fertility. In the respiratory system its role extends to aiding clearance of mucus through ciliary motion thereby helping maintain respiratory function.

Pathways

SPAG17 participates in the pathways regulating ciliary motion and spermatogenesis. The involvement of SPAG17 in these pathways highlights its relationships with other axonemal components such as dynein heavy chains which are responsible for motor activity along microtubules. Additionally it fits into the broader network of proteins governing microtubule dynamics working in concert to achieve its structural and functional roles.

SPAG17 influences conditions related to ciliary dysfunction such as primary ciliary dyskinesia. This disorder arises from defects in the motility of cilia manifesting in respiratory issues and potentially reduced fertility. The protein's relationship with other axonemal proteins like dynein motors links SPAG17 mutations to these symptoms. Additionally alterations in SPAG17 have been explored in research related to male infertility with studies suggesting that impaired sperm motility due to SPAG17 function can contribute to this condition.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the central pair apparatus of ciliary axonemes. Plays a critical role in the function and structure of motile cilia. May play a role in endochondral bone formation, most likely because of a function in primary cilia of chondrocytes and osteoblasts (By similarity). Essential for normal spermatogenesis and male fertility (By similarity). Required for normal manchette structure, transport of proteins along the manchette microtubules and formation of the sperm head and flagellum (By similarity). Essential for sperm flagellum development and proper assembly of the respiratory motile cilia central pair apparatus, but not the brain ependymal cilia (By similarity).
See full target information SPAG17
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Asian journal of andrology 27:245-253 PubMed39686771

2024

Novel homozygous SPAG17 variants cause human male infertility through multiple morphological abnormalities of spermatozoal flagella related to axonemal microtubule doublets.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Fazal Rahim,Meng-Lei Yang,Meftah Uddin,Jing-Wei Ye,Imtiaz Ali,Yousaf Raza,Abu Mansoor,Muhammad Shoaib,Mujahid Hussain,Ihsan Khan,Basit Shah,Asad Khan,Ahmad Nisar,Hui Ma,Bo Xu,Wasim Shah,Qing-Hua Shi

Journal of Cancer 8:443-454 PubMed28261346

2017

The Role of GOLPH3L in the Prognosis and NACT response in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling Feng,Fan He,Shumei Yan,He Huang,Qidan Huang,Ting Deng,Huini Wu,Bei Gao,Jihong Liu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB183089

Anti-spag1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-spag1 antibody (AB183089)

  • 0
  • 0
Primary Antibodies

AB121952

Anti-KIF26B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF26B antibody (AB121952)

  • 0
  • 0
Primary Antibodies

AB121991

Anti-HENMT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HENMT1 antibody (AB121991)

  • 0
  • 0
Primary Antibodies

AB121973

Anti-MYOM3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MYOM3 antibody (AB121973)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com