AB327130

Anti-SPAG5 Antibody [CL10078]

Name: Anti-SPAG5 Antibody [CL10078] (ab327130) | Abcam
Brand: Abcam Limited
SKU: AB327130
Price: 600 USD
Availability: InStock

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Mouse Monoclonal Astrin/Deepest antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Sperm-associated antigen 5.

View Alternative Names

Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126, SPAG5

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG5 Antibody [CL10078] (AB327130)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG5 Antibody [CL10078] (AB327130)

Immunohistochemical staining of human tonsil using ab327130 at 1 : 500–1 : 1000 dilution, showing strong cytoplasmic positivity in non-germinal center cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG5 Antibody [CL10078] (AB327130)

Immunohistochemical staining of human testis using ab327130 at 1 : 500–1 : 1000 dilution, showing moderate cytoplasmic positivity in cells in seminiferous ducts.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG5 Antibody [CL10078] (AB327130)

Immunohistochemical staining of human prostate using ab327130 at 1 : 500–1 : 1000 dilution, showing no cytoplasmic positivity in glandular cells as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG5 Antibody [CL10078] (AB327130)

Immunohistochemistry analysis in human testis and endometrium tissues using ab327130 at 1 : 500–1 : 1000 dilution. Corresponding SPAG5 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAG5 Antibody [CL10078] (AB327130)

Immunohistochemical staining of human endometrium using ab327130 at 1 : 500–1 : 1000 dilution, showing very weak cytoplasmic positivity in glandular cells as expected.

Supplier Data

Western blot - Anti-SPAG5 Antibody [CL10078] (AB327130)

Western blot analysis in human cell line RH-30 using ab327130 at 1 µg/ml.

All lanes:

Western blot - Anti-SPAG5 Antibody [CL10078] (ab327130)

All lanes:

Human cell line RH-30

Predicted band size: 134.42 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL10078

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Sperm-associated antigen 5.

Q96R06

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed : 11724960, PubMed : 12356910, PubMed : 27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed : 17664331, PubMed : 27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed : 18361916, PubMed : 21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed : 26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed : 23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed : 18055457).

See full target information Sperm-associated antigen 5

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Product promise

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