JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181187

Anti-SPAG7 antibody [EPR13391]

1

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal SPAG7 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Sperm-associated antigen 7, SPAG7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SPAG7 antibody [EPR13391] (AB181187)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPAG7 antibody [EPR13391] (AB181187)

Immunofluorescent analysis of MCF7 cells labeling SPAG7 with ab181187 at 1/250 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).

Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] (AB181187)
  • IP

Supplier Data

Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] (AB181187)

Immunoprecipitation. ab181187 at 1/1000 labeling SPAG7 immunoprecipitated from HeLa cell lysate using ab181187 at 1/50.

Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] (ab181187)

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Western blot - Anti-SPAG7 antibody [EPR13391] (AB181187)
  • WB

Supplier Data

Western blot - Anti-SPAG7 antibody [EPR13391] (AB181187)

All lanes:

Western blot - Anti-SPAG7 antibody [EPR13391] (ab181187) at 1/5000 dilution

Lane 1:

MCF-7 cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 26 kDa

false

  • Carrier free

    Anti-SPAG7 antibody [EPR13391] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13391

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB279649

Anti-BLBP antibody [EPR24033-13]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLBP antibody [EPR24033-13] (AB279649)

  • 5
  • 3
Primary Antibodies

AB252881

Anti-GFP antibody [3H9]

BOND RX™ Validated|Recombinant|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (AB252881)

  • 0
  • 0
Primary Antibodies

AB174854

Anti-WNK1 antibody [EPR2743(3)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-WNK1 antibody [EPR2743(3)] (AB174854)

  • 0
  • 0
Primary Antibodies

AB181083

Anti-FRG1 antibody [EPR13098]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-FRG1 antibody [EPR13098] (AB181083)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPAG7 also known as Sperm Associated Antigen 7 is a protein with an approximate mass of 26 kilodaltons. It is mainly expressed in the testis with additional expression noted in other tissues at lower levels. Mechanically SPAG7 functions in the regulation of cellular processes associated with spermatogenesis playing a role in the assembly and reorganization of microtubule structures.
Biological function summary

SPAG7 enhances the development and function of sperm cells contributing significantly to male fertility. The protein participates in microtubule-related activities and likely interacts with other proteins to perform its roles effectively. Research suggests that SPAG7 does not form a complex on its own but it closely associates with other cellular components in sperm maturation.

Pathways

SPAG7 interacts with regulatory networks involved in cilia and flagella movement contributing to the sperm's motility. SPAG7 shows a relationship with proteins like dynein and kinesin which are important in microtubule dynamics and cellular transport processes. It plays a part in pathways such as the spermatogenesis pathway where it ensures proper function and motility of sperm cells through its interactions with other proteins.

SPAG7 has associations with male infertility which is often linked to defective spermatogenesis and sperm motility issues. Abnormal SPAG7 expression may disrupt cellular transport processes causing infertility. Although not directly implicated in diseases like cancer alterations in SPAG7 levels could potentially influence cancer-associated proteins through indirect pathways reflecting complex biological interactions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information SPAG7
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 22:73 PubMed40082995

2025

Integrative analysis of serum proteomics and transcriptomics in hepatitis C.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqiong Wang,Andong Xia,Min Tang,Shengjun Yang,Yandi Shen,Jinhua Dao,Rui Tao,Wei Yue
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com