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AB250412

Anti-SPAG7 antibody [EPR13391] - BSA and Azide free

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Rabbit Recombinant Monoclonal SPAG7 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

Sperm-associated antigen 7, SPAG7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (AB250412)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (AB250412)

This data was developed using ab181187, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF7 cells labeling SPAG7 with ab181187 at 1/250 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).

Western blot - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (AB250412)
  • WB

Supplier Data

Western blot - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (AB250412)

This data was developed using ab181187, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SPAG7 antibody [EPR13391] (<a href='/en-us/products/primary-antibodies/spag7-antibody-epr13391-ab181187'>ab181187</a>) at 1/5000 dilution

Lane 1:

MCF-7 cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 26 kDa

false

Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (AB250412)
  • IP

Supplier Data

Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (AB250412)

This data was developed using ab181187, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab181187 at 1/1000 labeling SPAG7 immunoprecipitated from HeLa cell lysate using ab181187 at 1/50.

Lane 2 : Control IgG.

All lanes:

Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] (<a href='/en-us/products/primary-antibodies/spag7-antibody-epr13391-ab181187'>ab181187</a>)

Predicted band size: 26 kDa

Observed band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13391

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250412 is the carrier-free version of ab181187.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPAG7 also known as Sperm Associated Antigen 7 is a protein with an approximate mass of 26 kilodaltons. It is mainly expressed in the testis with additional expression noted in other tissues at lower levels. Mechanically SPAG7 functions in the regulation of cellular processes associated with spermatogenesis playing a role in the assembly and reorganization of microtubule structures.
Biological function summary

SPAG7 enhances the development and function of sperm cells contributing significantly to male fertility. The protein participates in microtubule-related activities and likely interacts with other proteins to perform its roles effectively. Research suggests that SPAG7 does not form a complex on its own but it closely associates with other cellular components in sperm maturation.

Pathways

SPAG7 interacts with regulatory networks involved in cilia and flagella movement contributing to the sperm's motility. SPAG7 shows a relationship with proteins like dynein and kinesin which are important in microtubule dynamics and cellular transport processes. It plays a part in pathways such as the spermatogenesis pathway where it ensures proper function and motility of sperm cells through its interactions with other proteins.

SPAG7 has associations with male infertility which is often linked to defective spermatogenesis and sperm motility issues. Abnormal SPAG7 expression may disrupt cellular transport processes causing infertility. Although not directly implicated in diseases like cancer alterations in SPAG7 levels could potentially influence cancer-associated proteins through indirect pathways reflecting complex biological interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SPAG7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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