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AB196596

Anti-SPAM1 antibody

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(6 Publications)

Anti-SPAM1 antibody (ab196596) is a rabbit polyclonal antibody detecting SPAM1 in Western Blot, ICC/IF. Suitable for Human.

View Alternative Names

HYAL3, PH20, SPAM1, Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-SPAM1 antibody (AB196596)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPAM1 antibody (AB196596)

Immunofluorescence analysis of Hela cells labelling SPAM1 using ab196596 at 1/50 (top) with DAPI blue for nuclear staining (bottom)

Western blot - Anti-SPAM1 antibody (AB196596)
  • WB

Supplier Data

Western blot - Anti-SPAM1 antibody (AB196596)

All lanes:

Western blot - Anti-SPAM1 antibody (ab196596) at 1/500 dilution

Lane 1:

PC3 cell extract

Lane 2:

Raji cell extract

Lane 3:

BT474 cell extract

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPAM1. The exact immunogen used to generate this antibody is proprietary information.

P38567

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-SPAM1 antibody (ab196596) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of SPAM1?
Anti-SPAM1 (ab196596) specifically detects a band for SPAM1 (UniProt: P38567) at a molecular weight of 58kDa.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPAM1 also known as sperm adhesion molecule 1 or PH-20 refers to a glycoprotein found on the sperm cell surface. Its molecular mass is approximately 64 kDa. This protein is primarily expressed in the testes and epididymis where it plays a role in sperm maturation. SPAM1 possesses hyaluronidase activity breaking down hyaluronic acid present in the cumulus cell matrix of the female reproductive tract during fertilization. It aids sperm in penetrating the egg's outer layers which is important for successful fertilization.
Biological function summary

SPAM1 participates in sperm penetration through the zona pellucida an essential step in fertilization. It acts independently facilitating sperm passage by degrading surrounding extracellular matrices. SPAM1 while not part of a larger protein complex interacts directly with the female reproductive tract's biochemical structures. These interactions ensure an efficient sperm-vs-egg recognition process that aids in sperm-egg fusion. Its enzymatic activity is central to this sperm-egg binding and membrane fusion.

Pathways

SPAM1 contributes significantly to the fertilization pathway specifically the Acrosome Reaction. It interacts indirectly with proteins like ZP3 a zona pellucida protein that triggers the acrosome reaction necessary for sperm to penetrate the ovum. Additionally SPAM1 plays a minor role in the hyaluronan-mediated motility pathway interacting faintly with the extracellular matrix components to enhance sperm motility and penetration.

SPAM1 has been implicated in certain infertility cases particularly those involving the failure of sperm to penetrate the egg successfully. This condition might relate to defects or alterations in SPAM1 activity or expression. Moreover some studies suggest a possible link between SPAM1 and testicular cancer where aberrant expression could influence tumorigenesis. SPAM1 anomalies may associate with other proteins like EZH2 involved in various cancers suggesting broader functional implications beyond reproductive biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
See full target information SPAM1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Revista internacional de andrologia 22:50-58 PubMed39748770

2025

By ameliorating redox imbalance, patients of oligoasthenospermia increased sperm acrosin activity after smoking cessation in Chinese.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyuan Lin,Yangkun Feng,Yun Zhang,Qingwen He,Ninghan Feng

Journal of cellular and molecular medicine 27:2437-2447 PubMed37436074

2023

PSMC4 promotes prostate carcinoma progression by regulating the CBX3-EGFR-PI3K-AKT-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaifeng Liu,Shengmin Zhang,Yongzhan Gong,Panyan Zhu,Weigan Shen,Qi Zhang

Nature communications 13:7630 PubMed36494335

2022

SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Scott B Biering,Francielle Tramontini Gomes de Sousa,Laurentia V Tjang,Felix Pahmeier,Chi Zhu,Richard Ruan,Sophie F Blanc,Trishna S Patel,Caroline M Worthington,Dustin R Glasner,Bryan Castillo-Rojas,Venice Servellita,Nicholas T N Lo,Marcus P Wong,Colin M Warnes,Daniel R Sandoval,Thomas Mandel Clausen,Yale A Santos,Douglas M Fox,Victoria Ortega,Anders M Näär,Ralph S Baric,Sarah A Stanley,Hector C Aguilar,Jeffrey D Esko,Charles Y Chiu,John E Pak,P Robert Beatty,Eva Harris

bioRxiv : the preprint server for biology : PubMed34931188

2021

SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Scott B Biering,Francielle Tramontini Gomes de Sousa,Laurentia V Tjang,Felix Pahmeier,Richard Ruan,Sophie F Blanc,Trishna S Patel,Caroline M Worthington,Dustin R Glasner,Bryan Castillo-Rojas,Venice Servellita,Nicholas T N Lo,Marcus P Wong,Colin M Warnes,Daniel R Sandoval,Thomas Mandel Clausen,Yale A Santos,Victoria Ortega,Hector C Aguilar,Jeffrey D Esko,Charles Y Chui,John E Pak,P Robert Beatty,Eva Harris

Liver international : official journal of the International Association for the Study of the Liver 41:1033-1043 PubMed33347692

2021

Co-expression of IL-7 and PH20 promote anti-GPC3 CAR-T tumour suppressor activity in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xingcheng Xiong,Juanli Xi,Qian Liu,Cixiao Wang,Zeyou Jiang,Su-Yang Yue,Lei Shi,Yuping Rong

Journal of extracellular vesicles 8:1670893 PubMed31632619

2019

Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103 dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsun Hong,Yoon Kyoung Kim,Gi Beom Kim,Gi-Hoon Nam,Seong A Kim,Yoon Park,Yoosoo Yang,In-San Kim
View all publications
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Product promise

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