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AB119280

Anti-SPANX antibody

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(4 Publications)

Rabbit Polyclonal SPANX antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SPANXC aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Sperm protein associated with the nucleus on the X chromosome C, Cancer/testis antigen 11.3, Cancer/testis-associated protein CTp11, Nuclear-associated protein SPAN-Xc, SPANX family member C, CT11.3, SPANX-C, SPANXC

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPANX antibody (AB119280)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPANX antibody (AB119280)

ab119280, at 5 ug/ml, staining SPANX in formalin-fixed, paraffin-embedded Human testis tissue, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SPANXC aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9NY87

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPANX also referred to as Sperm Protein Associated with the Nucleus on the X chromosome is a protein involved in cellular mechanisms. This protein has an approximate mass of 12-14 kDa. Expression of SPANX occurs mainly in the testis and it localizes primarily to nuclear compartments in mature spermatozoa. The SPANX family of proteins includes several paralogs each with specific but similar roles within the reproductive system. It seems the structural features of SPANX such as its nuclear localization signals play a role in its capability to bind chromatin and influence nuclear events.
Biological function summary

SPANX proteins participate in spermatogenesis contributing to the process of sperm maturation. These proteins lack known enzymatic activities but associate with chromatin to support sperm packaging and genome stability. Evidence does not suggest that SPANX forms part of a larger complex but its functions enable critical chromatin condensation needed for proper sperm morphogenesis. By maintaining genome stability during sperm formation SPANX influences reproductive success.

Pathways

Proteins like SPANX support pathways related to chromatin organization and spermatogenesis. In spermatogenesis SPANX proteins work alongside transition proteins and protamines in facilitating chromatin remodeling. The role of SPANX intersects with pathways of chromatin restructuring notably linked to histones and DNA repair proteins. While it does not interact directly with well-characterized signaling pathways SPANX remains essential in localized chromatin pathway interactions during sperm cell development.

Proteins from the SPANX family show expression in certain cancers including melanoma and prostate cancer. These disorders often exhibit dysregulated chromatin dynamics where SPANX expression alters cellular chromatin states. Investigations link overexpression of SPANX to tumorigenicity likely due to its effects on chromatin structures and subsequent gene expression patterns. Additionally SPANX associates indirectly with proteins like MAGE-A that also modulate cellular growth and tumor-related pathways suggesting overlapping roles in cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information SPANXC
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 20:107 PubMed35850889

2022

Slc2a6 regulates myoblast differentiation by targeting LDHB.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Jiang,Ninghan Feng,Yizhou Zhou,Xianlong Ye,Rong Wang,Jingwei Zhang,Siyuan Cui,Siyu Ji,Yongquan Chen,Shenglong Zhu

Scientific reports 11:3583 PubMed33574425

2021

The multifunctional role of SPANX-A/D protein subfamily in the promotion of pro-tumoural processes in human melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Itziar Urizar-Arenaza,Aitor Benedicto,Arantza Perez-Valle,Nerea Osinalde,Vyacheslav Akimov,Iraia Muñoa-Hoyos,Jose Antonio Rodriguez,Aintzane Asumendi,Maria Dolores Boyano,Blagoy Blagoev,Irina Kratchmarova,Nerea Subiran

Molecular cancer research : MCR 18:1560-1573 PubMed32571981

2020

SPANX Control of Lamin A/C Modulates Nuclear Architecture and Promotes Melanoma Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ikrame Lazar,Bertrand Fabre,Yongmei Feng,Ali Khateb,Patrick Turko,Julia M Martinez Gomez,Dennie T Frederick,Mitchell P Levesque,Lea Feld,Gao Zhang,Tongwu Zhang,Brian James,Jeny Shklover,Emily Avitan-Hersh,Ido Livneh,Marzia Scortegagna,Kevin Brown,Ola Larsson,Ivan Topisirovic,Haguy Wolfenson,Meenhard Herlyn,Keith Flaherty,Reinhard Dummer,Ze'ev A Ronai

Scientific reports 10:5625 PubMed32221341

2020

SPANX-A/D protein subfamily plays a key role in nuclear organisation, metabolism and flagellar motility of human spermatozoa.

Applications

Unspecified application

Species

Unspecified reactive species

Itziar Urizar-Arenaza,Nerea Osinalde,Vyacheslav Akimov,Michele Puglia,Iraia Muñoa-Hoyos,Marta Gianzo,Jose Antonio Rodriguez,Teresa Ganzabal,Blagoy Blagoev,Irina Kratchmarova,Nerea Subiran
View all publications
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