Rabbit Recombinant Monoclonal SPANXD antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519), expandable thumbnail](https://content.abcam.com/products/images/spanxd-antibody-epr8902-bsa-and-azide-free-ab249519--western-blot-img78558.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519)")
![Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519), expandable thumbnail](https://content.abcam.com/products/images/spanxd-antibody-epr8902-bsa-and-azide-free-ab249519--western-blot-img94984.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Alternative names
SPANXE, SPANXD, Sperm protein associated with the nucleus on the X chromosome D, Cancer/testis antigen 11.4, Nuclear-associated protein SPAN-Xd, SPANX family member D, CT11.4, SPANX-D
Recommended products
Rabbit Recombinant Monoclonal SPANXD antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8902
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249519 is the carrier-free version of Anti-SPANXD antibody [EPR8902] ab169773.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPANXD also known as Sperm Protein Associated with the Nucleus on the X Chromosome Family Member D is a protein with a molecular weight of approximately 11 kDa. SPANXD shows expression mainly in the testis and has a specific role during spermatogenesis. This protein belongs to the SPANX gene family which is particularly known for its expression in male germ cells. Additionally SPANXD appears to localize in the nucleus where it may play a role in chromatin organization during sperm development.
- Biological function summary
SPANXD functions in facilitating the proper packaging and organization of sperm chromatin ensuring genomic integrity during reproduction. It likely contributes to the final stages of sperm maturation particularly affecting chromatin condensation through interaction with the nuclear matrix. Although not extensively reported as part of large protein complexes SPANXD may work in conjunction with other SPANX family members to achieve its functional roles during sperm cell development.
- Pathways
SPANXD plays a part in the reproductive cellular pathways by ensuring the integrity and proper compaction of DNA in sperm cells. It is involved in spermatogenesis where it helps regulate the chromatin condensation process critical for functional sperm cells. Related proteins involved in these pathways include other family members like SPANXA and SPANXB which together may facilitate coordinated events during sperm cell development.
- Associated diseases and disorders
Researchers link SPANXD to fertility issues particularly male infertility due to its sperm-specific expression and function. Abnormal SPANXD expression or dysfunction can result in improper sperm development. Its connection to SPANX family members suggests combined impacts on spermatogenesis where disruptions can lead to azoospermia or oligospermia. Understanding SPANXD's role could contribute to developing targeted treatments for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (ab249519), expandable thumbnail](https://content.abcam.com/products/images/spanxd-antibody-epr8902-bsa-and-azide-free-ab249519--western-blot-img78558.jpg "Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (ab249519)")
Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (ab249519)
This data was developed using Anti-SPANXD antibody [EPR8902] ab169773, the same antibody clone in a different buffer formulation.
Blocking buffer: 5% NFDM/TBST.
Diluting buffer: 1% BSA /TBST.
All lanes: Western blot - Anti-SPANXD antibody [EPR8902] (Anti-SPANXD antibody [EPR8902] ab169773) at 1/300 dilution
All lanes: Mouse testis tissue lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa
![Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (ab249519), expandable thumbnail](https://content.abcam.com/products/images/spanxd-antibody-epr8902-bsa-and-azide-free-ab249519--western-blot-img94984.jpg "Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (ab249519)")
Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (ab249519)
This data was developed using Anti-SPANXD antibody [EPR8902] ab169773, the same antibody clone in a different buffer formulation.
Blocking buffer: 5% NFDM/TBST.
Diluting buffer: 1% BSA /TBST.
All lanes: Western blot - Anti-SPANXD antibody [EPR8902] (Anti-SPANXD antibody [EPR8902] ab169773) at 1/2000 dilution
All lanes: Mouse testis tissue lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 11 kDa, 16 kDa
Observed band size: 13 kDa, 16 kDa
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