AB249519

Anti-SPANXD antibody [EPR8902] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SPANXD antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

View Alternative Names

SPANXE, SPANXD, Sperm protein associated with the nucleus on the X chromosome D, Cancer/testis antigen 11.4, Nuclear-associated protein SPAN-Xd, SPANX family member D, CT11.4, SPANX-D

2 Images

Lab

Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519)

This data was developed using ab169773, the same antibody clone in a different buffer formulation.

Blocking buffer : 5% NFDM/TBST.

Diluting buffer : 1% BSA /TBST.

All lanes:

Western blot - Anti-SPANXD antibody [EPR8902] (<a href='/en-us/products/primary-antibodies/spanxd-antibody-epr8902-ab169773'>ab169773</a>) at 1/2000 dilution

All lanes:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa,16 kDa

Observed band size: 13 kDa,16 kDa

false

Lab

Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519)

This data was developed using ab169773, the same antibody clone in a different buffer formulation.

Blocking buffer : 5% NFDM/TBST.

Diluting buffer : 1% BSA /TBST.

All lanes:

Western blot - Anti-SPANXD antibody [EPR8902] (<a href='/en-us/products/primary-antibodies/spanxd-antibody-epr8902-ab169773'>ab169773</a>) at 1/300 dilution

All lanes:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa

false

Unconjugated

Anti-SPANXD antibody [EPR8902]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8902

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249519 is the carrier-free version of ab169773.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPANXD also known as Sperm Protein Associated with the Nucleus on the X Chromosome Family Member D is a protein with a molecular weight of approximately 11 kDa. SPANXD shows expression mainly in the testis and has a specific role during spermatogenesis. This protein belongs to the SPANX gene family which is particularly known for its expression in male germ cells. Additionally SPANXD appears to localize in the nucleus where it may play a role in chromatin organization during sperm development.

Biological function summary

SPANXD functions in facilitating the proper packaging and organization of sperm chromatin ensuring genomic integrity during reproduction. It likely contributes to the final stages of sperm maturation particularly affecting chromatin condensation through interaction with the nuclear matrix. Although not extensively reported as part of large protein complexes SPANXD may work in conjunction with other SPANX family members to achieve its functional roles during sperm cell development.

Pathways

SPANXD plays a part in the reproductive cellular pathways by ensuring the integrity and proper compaction of DNA in sperm cells. It is involved in spermatogenesis where it helps regulate the chromatin condensation process critical for functional sperm cells. Related proteins involved in these pathways include other family members like SPANXA and SPANXB which together may facilitate coordinated events during sperm cell development.

Researchers link SPANXD to fertility issues particularly male infertility due to its sperm-specific expression and function. Abnormal SPANXD expression or dysfunction can result in improper sperm development. Its connection to SPANX family members suggests combined impacts on spermatogenesis where disruptions can lead to azoospermia or oligospermia. Understanding SPANXD's role could contribute to developing targeted treatments for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SPANXD

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPANXD antibody [EPR8902] - BSA and Azide free

Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519)

Western blot - Anti-SPANXD antibody [EPR8902] - BSA and Azide free (AB249519)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise