Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Images
![Western blot - Anti-SPARC antibody [EPR20121] (AB207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--western-blot-img158582.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPARC antibody [EPR20121] (AB207743)")
![Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] (AB207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--flow-cytometry-intracellular-img139259.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] (AB207743)")
![Western blot - Anti-SPARC antibody [EPR20121] (AB207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--western-blot-img142326.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPARC antibody [EPR20121] (AB207743)")
![Immunoprecipitation - Anti-SPARC antibody [EPR20121] (AB207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--immunoprecipitation-img53068.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-SPARC antibody [EPR20121] (AB207743)")
![Western blot - Anti-SPARC antibody [EPR20121] (AB207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--western-blot-img144474.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPARC antibody [EPR20121] (AB207743)")
Publications
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- Neoplasma 69:1101-11072022SPARC induces M2 polarization of macrophages to promote proliferation, migration, and angiogenesis of cholangiocarcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShi-Kang Deng,Yun Jin,Yan Jin,Jun-Feng WangPubMed 35951457
- Oncology letters 21:4852021SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKehua Pan,Xince Huang,Xiufen JiaPubMed 33968201
- Phytotherapy research : PTR 34:591-6002020Icariin inhibits proliferation, migration, and invasion of medulloblastoma DAOY cells by regulation of SPARC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Yang,Guifang LiPubMed 32011040
- Journal of gastroenterology and hepatology 34:1860-18682019Secreted protein acidic and rich in cysteine promotes epithelial-mesenchymal transition of hepatocellular carcinoma cells and acquisition of cancerstem cell phenotypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Jiang,Fengchao Liu,Yiying Wang,Jian GaoPubMed 31041810
- Oncology letters 15:4323-43292018MicroRNA-211 suppresses prostate cancer proliferation by targeting SPARC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Hao et. al.PubMed 29541199
- Oncotarget 8:104508-1045242017hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShitong Zhang et. al.PubMed 29262657
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/70 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Alternative names
ON, SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, BM-40
Recommended products
Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20121
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SPARC also known as Secreted Protein Acidic and Rich in Cysteine or osteonectin is a glycoprotein with a molecular mass of approximately 32 to 43 kDa. It is widely expressed in various tissues notably within the bone skin and extracellular matrix. SPARC influences cell-matrix interactions and modulates cellular functions such as proliferation and migration. The protein plays a role in tissue remodeling and wound healing by interacting with structural components of the matrix and regulating cell adhesion.
- Biological function summary
SPARC impacts processes related to cell communication and matrix dynamics. It does not form part of large complexes but functions through interactions with matrix molecules and receptors. SPARC regulates collagen fibrillogenesis and influences the bioavailability of growth factors. It further affects angiogenesis through its ability to alter cellular adhesion and spreading which impacts vascular development and repair processes.
- Pathways
SPARC integrates into regulatory cascades such as the WNT signaling and TGF-β pathways. These pathways are essential for cellular growth repair and differentiation. In the context of the TGF-β signaling pathway SPARC modulates interactions with proteins like integrins and collagens impacting fibrotic processes and matrix assembly.
- Associated diseases and disorders
Alterations in SPARC expression show associations with cancer and fibrosis. The protein functions in tumor progression where it can influence tumor cell interactions with the stroma and affect cell migration and invasion. Furthermore in fibrotic diseases SPARC regulates the deposition and organization of collagen contributing to tissue stiffening. Connections are evident between SPARC and other matrix proteins like fibronectin known for their roles in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-SPARC antibody [EPR20121] (ab207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--western-blot-img158582.jpg "Western blot - Anti-SPARC antibody [EPR20121] (ab207743)")
Western blot - Anti-SPARC antibody [EPR20121] (ab207743)
Lanes 1 - 2: Merged signal (red and green). Green - ab207743 observed at 35 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.ab207743 was shown to recognize SPARC in wild-type HAP1 cells as signal was lost at the expected MW in SPARC knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SPARC knockout samples were subjected to SDS-PAGE. ab207743 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SPARC antibody [EPR20121] (ab207743) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: SPARC knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 35 kDa
Observed band size: 35 kDa
![Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] (ab207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--flow-cytometry-intracellular-img139259.jpg "Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] (ab207743)")
Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] (ab207743)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling SPARCwith ab207743 at 1/70 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-SPARC antibody [EPR20121] (ab207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--western-blot-img142326.jpg "Western blot - Anti-SPARC antibody [EPR20121] (ab207743)")
Western blot - Anti-SPARC antibody [EPR20121] (ab207743)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2/3: 3 minutes.
The observed molecular weight is consistent with what has been described in the literature (PMID: 18442048).
All lanes: Western blot - Anti-SPARC antibody [EPR20121] (ab207743) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 2: A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg
Lane 3: Human bone giant cell tumor lysate at 20 µg
SecondaryLanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lane 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Predicted band size: 35 kDa
Observed band size: 43 kDa
![Immunoprecipitation - Anti-SPARC antibody [EPR20121] (ab207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--immunoprecipitation-img53068.jpg "Immunoprecipitation - Anti-SPARC antibody [EPR20121] (ab207743)")
Immunoprecipitation - Anti-SPARC antibody [EPR20121] (ab207743)
SPARC was immunoprecipitated from 0.35 mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab207743 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207743 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: A-375 whole cell lysate, 10μg (Input).
Lane 2: ab207743 IP in A-375 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab207743 in A-375 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-SPARC antibody [EPR20121] (ab207743)
Predicted band size: 35 kDa
Observed band size: 43 kDa
![Western blot - Anti-SPARC antibody [EPR20121] (ab207743), expandable thumbnail](https://content.abcam.com/products/images/sparc-antibody-epr20121-ab207743--western-blot-img144474.jpg "Western blot - Anti-SPARC antibody [EPR20121] (ab207743)")
Western blot - Anti-SPARC antibody [EPR20121] (ab207743)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SPARC antibody [EPR20121] (ab207743) at 1/1000 dilution
Lane 1: Human serum at 10 µg
Lane 2: Human brain lysate at 10 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Predicted band size: 35 kDa
Observed band size: 43 kDa
Exposure time: 5s
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