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AB225716

Anti-SPARC antibody [SP205]

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(13 Publications)

Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 13 publications.

View Alternative Names

ON, SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, BM-40

14 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human lung squamos cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human stomach adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed, paraffin-embedded human testis tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human renal cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human breast ductal carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human placenta tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-SPARC antibody [SP205] (AB225716)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SPARC antibody [SP205] (AB225716)

Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1 : 200 dilution (0.595 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human colon adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed paraffin-embedded human cerebellum tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (AB225716)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (AB225716)

Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1 : 15 (7.9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-SPARC antibody [SP205] (AB225716)
  • WB

Supplier Data

Western blot - Anti-SPARC antibody [SP205] (AB225716)

All lanes:

Western blot - Anti-SPARC antibody [SP205] (ab225716) at 1/400 dilution

Lanes 1 and 3:

HUVEC (human umbilical vein endothelial cell line) cell lysate

Lanes 2 and 4:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP205

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary incubation for 10 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p>Primary incubation for 1 hour at room temperature.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/15", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p>Primary incubation for 1 hour at room temperature.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p>Primary incubation for 1 hour at room temperature.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPARC also known as Secreted Protein Acidic and Rich in Cysteine or osteonectin is a glycoprotein with a molecular mass of approximately 32 to 43 kDa. It is widely expressed in various tissues notably within the bone skin and extracellular matrix. SPARC influences cell-matrix interactions and modulates cellular functions such as proliferation and migration. The protein plays a role in tissue remodeling and wound healing by interacting with structural components of the matrix and regulating cell adhesion.
Biological function summary

SPARC impacts processes related to cell communication and matrix dynamics. It does not form part of large complexes but functions through interactions with matrix molecules and receptors. SPARC regulates collagen fibrillogenesis and influences the bioavailability of growth factors. It further affects angiogenesis through its ability to alter cellular adhesion and spreading which impacts vascular development and repair processes.

Pathways

SPARC integrates into regulatory cascades such as the WNT signaling and TGF-β pathways. These pathways are essential for cellular growth repair and differentiation. In the context of the TGF-β signaling pathway SPARC modulates interactions with proteins like integrins and collagens impacting fibrotic processes and matrix assembly.

Alterations in SPARC expression show associations with cancer and fibrosis. The protein functions in tumor progression where it can influence tumor cell interactions with the stroma and affect cell migration and invasion. Furthermore in fibrotic diseases SPARC regulates the deposition and organization of collagen contributing to tissue stiffening. Connections are evident between SPARC and other matrix proteins like fibronectin known for their roles in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
See full target information SPARC
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40429837

2025

Biosynthesized Calcium Peroxide Nanoparticles as a Multifunctional Platform for Liver Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Wu,Siqi Li,Xin Xia,Gen Zhang,Ting Wang

Inflammation and regeneration 44:44 PubMed39482755

2024

SPARC activates p38γ signaling to promote PFKFB3 protein stabilization and contributes to keloid fibroblast glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yining Liu,Wei Zhang,Nan Lin,Zelei Yang,Yanxin Liu,Huaxia Chen

Cell proliferation 57:e13710 PubMed39010274

2024

Compromised cell competition exhausts neural stem cells pool.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxiao Li,Mengtian Zhang,Yushan Du,Shuang Liu,Da Li,Shukui Zhang,Fen Ji,Jingjing Zhang,Jianwei Jiao

Acta neuropathologica communications 11:84 PubMed37217978

2023

Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS function.

Applications

Unspecified application

Species

Unspecified reactive species

Luise A Seeker,Nadine Bestard-Cuche,Sarah Jäkel,Nina-Lydia Kazakou,Sunniva M K Bøstrand,Laura J Wagstaff,Justyna Cholewa-Waclaw,Alastair M Kilpatrick,David Van Bruggen,Mukund Kabbe,Fabio Baldivia Pohl,Zahra Moslehi,Neil C Henderson,Catalina A Vallejos,Gioele La Manno,Goncalo Castelo-Branco,Anna Williams

Journal of tissue engineering 13:20417314221140500 PubMed36582941

2022

RANKL neutralisation prevents osteoclast activation in a human ameloblastoma-bone model.

Applications

Unspecified application

Species

Unspecified reactive species

Judith Pape,Deniz Bakkalci,Rawiya Al Hosni,Benjamin S Simpson,Kristiina Heikinheimo,Stefano Fedele,Umber Cheema

Matrix biology plus 16:100125 PubMed36452176

2022

Modelling stromal compartments to recapitulate the ameloblastoma tumour microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Bakkalci,Amir Zaki Abdullah Zubir,Syed Ali Khurram,Judith Pape,Kristiina Heikinheimo,Stefano Fedele,Umber Cheema

Frontiers in endocrinology 13:869519 PubMed35721704

2022

Secreted Protein Acidic and Rich in Cysteine Mediates the Development and Progression of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Luo,Xi Sun,Min Tang,Jiahui Wu,Tianwei Qian,Shimei Chen,Zhiyuan Guan,Yanyun Jiang,Yang Fu,Zhi Zheng

Neoplasma 69:794-806 PubMed35400166

2022

Methylation-mediated expression of SPARC is correlated with tumor progression and poor prognosis of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Li,Jia-Li Li,Dong-Jie Qiu,Li Ma

Cancer management and research 13:8929-8951 PubMed34876855

2021

Bioinformatics Analysis of Hub Genes and Potential Therapeutic Agents Associated with Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Zhang,Xuelian Xiang,Li Liu,Huiying Yang,Dongliang Cen,Guodu Tang

Kidney & blood pressure research 46:588-600 PubMed34284400

2021

Tumor-Suppressing STF cDNA 3 Overexpression Suppresses Renal Fibrosis by Alleviating Anoikis Resistance and Inhibiting the PI3K/Akt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xiao,Xinghong Liu,Yan Chen,Huanzi Dai
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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