Anti-SPARC antibody [SP205] Rabbit monoclonal (ab225716)

Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	WB	ICC/IF
Human	Tested	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended
Cow	Predicted	Predicted	Not recommended	Predicted
Pig	Predicted	Predicted	Not recommended	Predicted
Rabbit	Predicted	Predicted	Not recommended	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Primary incubation for 10 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit, Cow, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit, Cow, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/400	Notes Primary incubation for 1 hour at room temperature.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Primary incubation for 1 hour at room temperature.
Species Rat	Dilution info -	Notes Primary incubation for 1 hour at room temperature.
Species Rabbit	Dilution info -	Notes -
Species Cow	Dilution info -	Notes -
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/15	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rabbit, Cow, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information SPARC

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Alternative names

ON, SPARC, Basement-membrane protein 40, Osteonectin, Secreted protein acidic and rich in cysteine, BM-40

Recommended products

Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: SP205
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SPARC also known as Secreted Protein Acidic and Rich in Cysteine or osteonectin is a glycoprotein with a molecular mass of approximately 32 to 43 kDa. It is widely expressed in various tissues notably within the bone skin and extracellular matrix. SPARC influences cell-matrix interactions and modulates cellular functions such as proliferation and migration. The protein plays a role in tissue remodeling and wound healing by interacting with structural components of the matrix and regulating cell adhesion.

Biological function summary

SPARC impacts processes related to cell communication and matrix dynamics. It does not form part of large complexes but functions through interactions with matrix molecules and receptors. SPARC regulates collagen fibrillogenesis and influences the bioavailability of growth factors. It further affects angiogenesis through its ability to alter cellular adhesion and spreading which impacts vascular development and repair processes.

Pathways

SPARC integrates into regulatory cascades such as the WNT signaling and TGF-β pathways. These pathways are essential for cellular growth repair and differentiation. In the context of the TGF-β signaling pathway SPARC modulates interactions with proteins like integrins and collagens impacting fibrotic processes and matrix assembly.

Associated diseases and disorders

Alterations in SPARC expression show associations with cancer and fibrosis. The protein functions in tumor progression where it can influence tumor cell interactions with the stroma and affect cell migration and invasion. Furthermore in fibrotic diseases SPARC regulates the deposition and organization of collagen contributing to tissue stiffening. Connections are evident between SPARC and other matrix proteins like fibronectin known for their roles in these pathological conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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14 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human kidney tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (ab225716)
Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1:15 (7.9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-SPARC antibody [SP205] (ab225716)
All lanes: Western blot - Anti-SPARC antibody [SP205] (ab225716) at 1/400 dilution
Lanes 1 and 3: HUVEC (human umbilical vein endothelial cell line) cell lysate
Lanes 2 and 4: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human testis tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human lung squamos cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human placenta tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry (Intracellular) - Anti-SPARC antibody [SP205] (ab225716)
Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1:200 dilution (0.595 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.