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Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (AB225716), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (AB225716), expandable thumbnail
  • Western blot - Anti-SPARC antibody [SP205] (AB225716), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)WBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Cow
Predicted
Predicted
Not recommended
Predicted
Pig
Predicted
Predicted
Not recommended
Predicted
Rabbit
Predicted
Predicted
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Primary incubation for 10 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rabbit, Cow, Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Predicted
Predicted

Species

Rabbit, Cow, Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

Primary incubation for 1 hour at room temperature.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Primary incubation for 1 hour at room temperature.

Species

Rat

Dilution info

-

Notes

Primary incubation for 1 hour at room temperature.

Species

Rabbit

Dilution info

-

Notes

-

Species

Cow

Dilution info

-

Notes

-

Species

Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/15

Notes

-

Predicted
Predicted

Species

Rabbit, Cow, Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SPARC antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP205

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (ab225716)

    Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1:15 (7.9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Western blot - Anti-SPARC antibody [SP205] (ab225716)

    All lanes: Western blot - Anti-SPARC antibody [SP205] (ab225716) at 1/400 dilution

    Lanes 1 and 3: HUVEC (human umbilical vein endothelial cell line) cell lysate

    Lanes 2 and 4: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human testis tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human lung squamos cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

    Formalin-fixed, paraffin-embedded human cerebellum tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-SPARC antibody [SP205] (ab225716), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SPARC antibody [SP205] (ab225716)

    Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1:200 dilution (0.595 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com