AB255597

Anti-SPARCL1 antibody [EPR22615-277]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal SPARCL1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications.

View Alternative Names

SPARC-like protein 1, High endothelial venule protein, MAST 9, Hevin, SPARCL1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling SPARCL1 with ab255597 at 1/1000 dilution (0.623 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human cerebral cortex (PMID : 18381651, 9199668). The section was incubated with ab255597 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling SPARCL1 with ab255597 at 1/1000 dilution (0.623 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in high endothelial venules of human tonsil (PMID : 8626806). The section was incubated with ab255597 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Unknown

Western blot - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

SPARCL1 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID : PMID : 22891198). Negative control : human liver (PMID : 9485012).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SPARCL1 antibody [EPR22615-277] (ab255597) at 1/1000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Lane 3:

Human lung lysate at 20 µg

Lane 4:

Human testis lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 75 kDa

false

Exposure time: 15s

Carrier free

Anti-SPARCL1 antibody [EPR22615-277] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22615-277

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SPARCL1 also known as Hevin or Secreted Protein Acidic and Rich in Cysteine-Like 1 is a protein with a mass of approximately 75 kDa. It is part of the SPARC (Secreted Protein Acidic and Rich in Cysteine) family of matricellular proteins. SPARCL1 is mainly expressed in various tissues including the heart brain lungs and skeletal muscle. This protein exhibits strong conservation across species hinting at its importance to fundamental biological processes.

Biological function summary

SPARCL1 regulates cell-matrix interactions. It is not part of a protein complex but it interacts with other extracellular matrix components to influence cell adhesion and migration. SPARCL1 plays a role in normal homeostasis and is involved in the control of the extracellular matrix organization. It affects cellular behavior by modulating the activity of matrix metalloproteinases and influences the adhesion properties of cells by binding to components even in absence of complex formation.

Pathways

SPARCL1 impacts cellular processes like tissue remodeling and inflammation. It operates within the ECM remodeling and focal adhesion pathways. In ECM remodeling related proteins such as MMPs (Matrix Metalloproteinases) interact with SPARCL1 affecting tissue integrity and repair. In the focal adhesion pathway SPARCL1 affects cell shape and movement by regulating interactions with integrins which are integral membrane proteins that communicate the extracellular matrix to the cell.

SPARCL1 has associations with cancer and neuropathological diseases. Reduced expression of SPARCL1 often occurs in various cancers including prostate and colorectal cancers suggesting its potential role as a tumor suppressor. In neuropathological conditions such as Alzheimer's disease altered SPARCL1 expression affects neuroinflammation and synaptic functions related to the amyloid precursor protein (APP) which plays a critical role in Alzheimer's pathology. Understanding these pathways offers insight into therapeutic potential in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information SPARC-like protein 1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 23:368 PubMed39529018

2024

NAT10 promotes liver lipogenesis in mouse through N4-acetylcytidine modification of Srebf1 and Scap mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouqi Wang,Xinxing Wan,Md Asaduzzaman Khan,Lin Peng,Xiaoying Sun,Xuan Yi,Ke Chen

Oncology letters 28:587 PubMed39411205

2024

Revolutionizing prognostic predictions in colorectal cancer: Macrophage‑driven transcriptional insights from single‑cell RNA sequencing and gene co‑expression network analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Feng,Zhuo Cheng,Jingyuan Gao,Tao Huang,Jun Wang,Qian Tang,Ke Pu,Chang Liu

Histology and histopathology 39:483-496 PubMed37534667

2023

microRNA-105-5p protects against chondrocyte injury, extracellular matrix degradation, and osteoarthritis progression by targeting SPARCL1.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Jiang,Shigao Cheng,Pengcheng Kang,Tengfei Li,Xun Li,Jiongzhe Xiao,Lian Ren

Cancer cell international 21:259 PubMed33980221

2021

Low expression of CHRDL1 and SPARCL1 predicts poor prognosis of lung adenocarcinoma based on comprehensive analysis and immunohistochemical validation.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Deng,Qingqing Hang,Dijian Shen,Yibi Zhang,Ming Chen

Translational oncology 14:100981 PubMed33395744

2021

Integrative single-cell transcriptome analysis reveals a subpopulation of fibroblasts associated with favorable prognosis of liver cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyang Wang,Chao Feng,Meixin Lu,Biao Zhang,Yingchen Xu,Quan Zeng,Jiafei Xi,Junnian Zhou,Xiaomin Ying,Jian Zhang,Wen Yue,Xuetao Pei

Cytotechnology 73:71-78 PubMed33505115

2021

The inhibition of HeLa cells proliferation through SPARCL1 mediated by SPP1.

Applications

Unspecified application

Species

Unspecified reactive species

Shengpeng Zhang,Fengge Zhang,Limin Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPARCL1 antibody [EPR22615-277]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

Western blot - Anti-SPARCL1 antibody [EPR22615-277] (AB255597)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise